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Design of actuator and control system for kelp automatic knotting machine HUANG Guifang, LIN Jianwei, WEI Guanyuan, DING Lan, CAI Wenhong, WEI Shengjun Journal of Fisheries Research    2023, 45 (6): 585-592.   DOI: 10.14012/j.cnki.fjsc.2023.06.009 Abstract （289）   HTML （23）    PDF （4468KB）（418）       Knowledge map    Save The kelp knots are the main product for exporting and earning foreign exchange in Chinese kelp culture provinces, but there are no mechanical processing devices. Therefore, the kelp knots are usually tied manually. Based on the principle of bionics, this paper offered an automatic knotting machine which could make the kelp forming a circle and passing through it to form a knot with the logic circuit. The test verified that this knotting machine was able to complete the operational requirements for kelp knotting accurately, which showed that this design was reasonable and feasible, and had the value of promotion and application after further optimization. It could also provide certain reference for the design and application of relevant knotting equipment. Table and Figures | Reference | Related Articles | Metrics

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Development status and countermeasure analysis of mainstream low carbon aquaculture technology at home and abroad under the double carbon background FENG Ying, ZENG Ya, REN Tongjun Journal of Fisheries Research    2023, 45 (6): 603-613.   DOI: 10.14012/j.cnki.fjsc.2023.06.011 Abstract （268）   HTML （20）    PDF （1712KB）（335）       Knowledge map    Save Carbon peaking and carbon neutrality are important measures to address climate change in China. Carbon emission reduction in aquaculture is crucial to achieving the dual carbon target. Therefore, studying and exploring low-carbon aquaculture technology has important practical significance. Based on the concept of low carbon development and the background of dual carbon goals, the present study analyzed the current situation and trends of low carbon aquaculture technology development at home and abroad, discussed the urgent issues to be solved in the development of low carbon aquaculture technology in China, and proposed relevant countermeasures in terms of innovation of low carbon technology, improvement of policies and regulations, and construction of professional personnel in combination with China’s national conditions, aiming at effectively reducing greenhouse gas emissions from China’s aquaculture industry,promoting high-quality green transformation of aquaculture industry to provide scientific reference and contribute to the realization of China’s dual carbon vision. Table and Figures | Reference | Related Articles | Metrics

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Advances of genetic engineering modification in Chlorella ZHANG Tiantian, CHEN Bilian, ZHENG Mingmin Journal of Fisheries Research    2023, 45 (6): 593-602.   DOI: 10.14012/j.cnki.fjsc.2023.06.010 Abstract （221）   HTML （17）    PDF （1302KB）（378）       Knowledge map    Save Chlorella is a unicellular microalga that could grow and accumulate biomass through photosynthesis using sunlight and carbon dioxide. It is widely used to produce biofuels and other high-value products in fields like aquaculture, food, energy, and the environment. With the development of genetic engineering technology, research and applications of Chlorella at the molecular level have received increasing attention, making specific genetic modification possible. In this paper, we summarized and discussed the application of Chlorella in genetic engineering and protein production from four perspectives: transformation system, transformation methods, factors affecting transformation and expression of exogenous proteins. The aim of this review was to provide theoretical support and references for high-value products production of Chlorella. Table and Figures | Reference | Related Articles | Metrics

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Pathological analysis of scuticociliatosis of Larimichthys crocea in indoor circulating water culture YANG Guiqin, LI Xiaodong, LU Weijing, JIANG Fan, CHEN Ziqin, ZHANG Weini, CHEN Xinhua Journal of Fisheries Research    2023, 45 (6): 523-529.   DOI: 10.14012/j.cnki.fjsc.2023.06.001 Abstract （220）   HTML （12）    PDF （1951KB）（359）       Knowledge map    Save In order to investigate the common fish diseases in indoor culture of large yellow croaker (L.crocea), this study reports the occurrence of scuticociliatosis, a parasitic disease, on the surface of L.crocea in a laboratory circulating water recirculation system. The histopathological analysis of skin, liver and muscle of L.crocea was carried out to explore the pathogenic mechanism of scuticociliatosis. At the same time, in order to further identify the body surface parasitic Scuticociliatida ciliates of L.crocea, the pathogen was isolated, and the morphological characteristics of Scuticociliatida ciliates were analyzed by in vivo formaldehyde fixed microscopic observation and scanning electron microscope observation. The results revealed the presence of whitening symptoms on the head, skin, and fin base of L.crocea. Gill cover congestion and severe cases of white massive skin ulceration were observed, along with the infestation of a significant number of Scuticociliatida ciliates. Notably, no apparent pathological alterations were detected in the body tissue. Histopathological analysis showed that the skin and subcutaneous tissue were the main sites of infection with Scuticociliatida ciliates. Tissue necrosis, cytolysis and increase of inflammatory cells occurred in most areas of muscle tissue. Myofibril was obviously broken and muscle fiber space was enlarged, while the boundary between liver tissue cells was not obvious, the central vein was oppressed, mononuclear cells increased and parenchyma cells enlarged. It was observed that the body of the pathogen was mainly slightly pointed at the top, blunt and round at the back end of the body, and contained an obvious telescopic vesicle as well as a long flagellum. The pathogen was identified as Pseudocohnilembus persalinus by morphology. Taken together, the histopathological results showed that the Scuticociliatida ciliates could infect the body surface tissue of L.crocea, resulting in loss of protection and resistance. At the same time, this study can provide a certain reference basis for indoor healthy culture of L.crocea. Table and Figures | Reference | Related Articles | Metrics

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Analysis and suggestions for promotion on tail water treatment mode of eel culture in Fujian Province ZHONG Guolong Journal of Fisheries Research    2024, 46 (1): 92-100.   DOI: 10.14012/j.cnki.fjsc.2024.01.010 Abstract （199）   HTML （9）    PDF （1794KB）（570）       Knowledge map    Save With the acceleration of China’s ecological civilization construction,the fishery has entered a new era of high-quality development.Eel,as one of the main freshwater aquaculture species of Fujian Province,the problem of its breeding tail water discharge has become increasingly prominent.Four primary treatment modes for eel culture in Fujian Province are currently employed.These modes include the conventional chemical treatment,the improved chemical treatment in industrial circulating water eel culture,the ecological comprehensive planting and breeding treatment,and the double circulation zero discharge eel culture treatment.This paper elucidates the primary technical processes and mode characteristics of eel culture tail water treatment.It also analyzes the key advantages and disadvantages of these processes and modes,and presents recommendations to promote future advancements in eel culture tail water treatment.The findings of this study serve as a valuable reference for enhancing aquaculture tail water treatment technology in Fujian Province,as well as nationwide. Table and Figures | Reference | Related Articles | Metrics

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Investigation of fish resources in the headwaters of Chishui River CHEN Wenshan, CHEN Bolin, LU Qun, LIU Jianhu, HE Tao, YANG Feng Journal of Fisheries Research    2024, 46 (1): 60-68.   DOI: 10.14012/j.cnki.fjsc.2024.01.007 Abstract （194）   HTML （10）    PDF （891KB）（276）       Knowledge map    Save To monitor fish resources and biodiversity in the headwaters of Chishui River,a comprehensive investigation was conducted using both traditional catch methods and environmental DNA (eDNA) technology from 2020 to 2021.A total of 48 fish species were identified,representing 4 orders,10 families,and 41 genera.Traditional fishing methods captured 23 fish species,spanning 2 orders,5 families,and 22 genera,while eDNA technology expanded the detection to encompass 4 orders,8 families,38 genera,and 40 species.In the analysis of fish species composition,the relative importance index (IRI) highlighted Schizothorax grahami,Acrossocheilus yunnanensis,Zacco platypus,and Semilabeo prochilus as the dominant species in the river section of the reserve.The Shannon-Wiener index registered at 2.99,indicating relatively rich overall fish resources and a relatively stable community structure.The catch Margalef index (D) reached 3.31,affirming the richness of fish species in the monitoring area,with a stable composition and population structure.Further assessment revealed a catch evenness index of 0.66,indicating a uniform distribution of population structure.Notably,differences in fish resources between the main river and tributaries were observed.Based on these findings,future conservation efforts should prioritize the core protection zone of the main stream,enhancing measures for fish diversity protection.Additionally,targeted interventions to restore tributary resources should be progressively implemented to elevate the overall resource level.This study provides valuable insights into the diverse fish species,their distribution,and recommendations for effective conservation strategies in the headwaters of Chishui River. Table and Figures | Reference | Related Articles | Metrics

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Histological observation on the gonadal development of the parental Thamnaconus modestus during early reproductive regulation MAO Xintong, LIU Liming, WANG Jiulong, LIU Yan, WU Yanqing, JIANG Hongli, XU Wengang Journal of Fisheries Research    2024, 46 (1): 11-18.   DOI: 10.14012/j.cnki.fjsc.2024.01.002 Abstract （188）   HTML （11）    PDF （2514KB）（335）       Knowledge map    Save This study aimed to understand the gonadal development and reproductive characteristics of the black scraper (T.modestus) before and after spawning.From November 2021 to March 2022,the parent fish were collected for five times,with three males and three females at each time.The total length,body weight,hepatopancreas index and gonadosomatic index were measured every time.At the same time,paraffin-embedded sections and hematoxylin-eosin staining were used to observe the gonadal development.The results showed that under artificial temperature control,the gonad weight and gonadosomatic index of the female and male parental T.modestus reached the maximum in February,and entered the peak period of sexual maturity,which was significantly higher than that in other months (P<0.05),suggesting that the parent fish reached the peak of maturity in February under the condition of artificially controlled water temperature.The spawning type of T.modestus was asynchronous and batch spawning,and the ovarian development of T.modestus could be divided into six phases,which was peri-nucleolus,yolk vesicle,primary yolk,secondary yolk,teritary yolk,and ripe stage,respectively.The testis development of T.odestus could be divided into five phases,which was spermatogonium,primary spermatocyte,secondary spermatocyte,spermatid and spermatozoa,respectively.In conclusion,this study investigated the morphological characteristics,organizational structure and developmental rules at different gonadal development stages of T.modestus,which may enrich the basic data of reproductive biology and provide scientific basis for artificial breeding and seed production of T.modestus. Table and Figures | Reference | Related Articles | Metrics

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Study on the differential expression of temperature tolerance related genes in Apostichopus japonicus between south-cultivated in Fujian Province and north-cultivated groups ZHU Zhihuang, LIN Qi, WU Jianshao, YANG Qiuhua Journal of Fisheries Research    2024, 46 (1): 1-10.   DOI: 10.14012/j.cnki.fjsc.2024.01.001 Abstract （185）   HTML （38）    PDF （2048KB）（183）       Knowledge map    Save In this study,juvenile A.japonicus (experimental group) were purchased from northern China and reared in southern China under natural water temperature for one year.The same age seedlings of juvenile A.japonicus (control group) were directly collected from northern China to compare their thermal resistance with those of the experimental group.The study of thermal limits showed that the sublethal temperature of A.japonicus in the experimental group was 32 °C,significantly higher than the 30 °C in the control group.The ULTs50 of A.japonicus in the experimental and control groups were 33.1 °C and 31.9 °C,respectively.These results demonstrated that the thermal history influenced the upper thermal limit of A.japonicus,and A.japonicus undergoing continuous thermal shock would possess stronger thermal resistance.Additionally,the expression patterns of HSP90a,gp96,HSP70,HSP26,and proeintl(2)efl genes after heat shock for 1,2,and 3 hours between the experimental and control groups were analyzed using qRT-PCR.The expression patterns of HSP90a,gp96,HSP70,HSP26,and proeintl(2)efl genes were different between the experimental and control groups.At 30 °C heat shock,the gene expressions of HSP90a,gp96,HSP70,and HSP26 in the control group did not noticeably increase after stimulation for 1 and 2 hours but were significantly up-regulated at 3 hours,with expression values reaching (9.801±1.303),(2.508±0.910),(8.649±1.936),and (34.787±4.978),respectively.However,all of the HSP90a,gp96,HSP70,and HSP26 gene expressions in the experimental group were dramatically up-regulated at 1 hour at 30 °C,with expression values reaching (42.000±8.798),(20.019±6.224),(218.750±78.701),and (93.710±5.674),respectively.These four temperature-tolerant related genes detected in the present study in the experimental group were up-regulated earlier than those in the control group,indicating a higher ability of A.japonicus in the experimental group to respond adaptively to heat shock than that in the control group.Therefore,the research showed that A.japonicus undergoing high temperature acclimation could respond earlier to external environmental heat conditions. Table and Figures | Reference | Related Articles | Metrics

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The research progress on the degradation mechanism of microcystins in water by environmental microbes ZHANG He, XU Wenchang, YUAN Yan, XU Jingyi, MA Yantian Journal of Fisheries Research    2024, 46 (3): 215-227.   DOI: 10.14012/j.jfr.2023125 Abstract （176）   HTML （25）    PDF （968KB）（167）       Knowledge map    Save Over the past few years, the issue of Cyanobacteria bloom pollution has escalated due to the combined impacts of global warming and eutrophication in aquatic systems. This has resulted in the release of toxic microcystins (MCs), posing a significant threat to human health and aquatic ecosystems. Effectively controlling and eliminating MCs from water bodies is a pressing global challenge. Fortunately, prior research has demonstrated that microbial degradation of MCs presents an efficient and environmentally friendly solution. This review explores the production, structure, and toxicity of MCs, with a focus on summarizing global advancements in enzymatic degradation pathways, degradation enzymes, and degradation genes associated with MCs. Additionally, it also analyzes the potential applications of microbial degradation bacteria in the ecological restoration of water bodies. Furthermore, the paper delves into future research directions, including alternative pathways for non-mlr-mediated MCs degradation mechanisms, optimizing the expression of MCs-degrading enzymes, and constructing highly efficient dual-functional degradation bacteria. These perspectives aim to deepen human’s understanding of microbial degradation mechanisms of MCs and provide innovative solutions to address the global challenge of MCs contamination in water bodies. Table and Figures | Reference | Related Articles | Metrics

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Development status and suggestions on the development of deep-sea aquaculture in Fuzhou, Fujian Province HUANG Yongqiang, YOU Xiaoting, YANG Xiaoqiang, CAI Leiming, LIN Jiemei, WANG Weigang, CHEN Fei Journal of Fisheries Research    2024, 46 (3): 302-310.   DOI: 10.14012/j.jfr.2023089 Abstract （172）   HTML （15）    PDF （1844KB）（218）       Knowledge map    Save Promoting the development of deep-sea aquaculture is an important measure to alleviate the pressure on the offshore aquaculture environment, optimize mariculture space, ensure the effective supplement of aquatic products, and promote the sustainable development of seawater aquaculture in Fuzhou.This paper introduces the development status of deep-sea anti-storm-wave gravity cage, deep-sea aquaculture platform, and property rights registration of deep-sea aquaculture platform. The development difficulties of deep-sea aquaculture of Fuzhou are pointed out. Some suggestions are made in this paper for the development of deep-sea aquaculture of Fuzhou, hoping to provide reference for the development of deep-sea aquaculture. Table and Figures | Reference | Related Articles | Metrics

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The effects of different diets on the growth, survival and nutritional composition of Artemia nauplii WU Shuiqing, WU Jianshao, GUO Shaopeng, WEN Ping, QIU Denggao, LI Leibin, LUO Huiyu Journal of Fisheries Research    2024, 46 (1): 29-37.   DOI: 10.14012/j.cnki.fjsc.2024.01.004 Abstract （166）   HTML （7）    PDF （1019KB）（245）       Knowledge map    Save Artemia is an important biological feed for aquatic animals.In this study,Saccharomyces cerevisiae,powder of Chlorella pyenoidosa,and fermented shrimp flake were fed to new hatched Artemia nauplii to evaluate the effects of different diets on the growth,survival,and nutritional composition of Artemia nauplii.Results showed that after 6 days feeding,the fermented shrimp flake feed group had the largest average body length,which were significantly larger than that of the S.cerevisiae group and the powder of C.pyenoidosa group (P<0.05).The survival rate of Artemia nauplii fed with S.cerevisiae was the lowest,but there were no significant differences between the powder of C.pyenoidosa group and the fermented shrimp flake group (P>0.05).After 6 days feeding,the crude protein content of Artemia nauplii significantly increased,but the crude fat content significantly decreased compared to Artemia nauplii (P<0.05).The protein content of Artemia nauplii in the powder of C.pyenoidosa group and the fermented shrimp flakes group was significantly higher than that in the S.cerevisiae group (P<0.05).Furthermore,the highest crude fat content was obtained in Artemia nauplii that were fed with the fermented shrimp flakes.The fermented shrimp flakes group had the highest total amino acid (TAA) content and essential amino acid (EAA) content.All experimental groups contained high levels of eicosapentaenoic acid (EPA),but the docosahexaenoic acid (DHA) was undetectable.The total fatty acid content and polyunsaturated fatty acid content in the fermented shrimp flakes group were significantly higher than those in the powder of C.pyenoidosa group and the S.cerevisiae group (P<0.05).However,the Artemia nauplii fed with powder of C.pyenoidosa had significantly higher high unsaturated fatty acid (HUFA) content and EPA content than the fermented shrimp flakes group as well as the S.cerevisiae group (P<0.05).The outcome of this study provided that fermented shrimp flakes and powder of C.pyenoidosa had advantages in enhancing the nutrition of Artemia,and could be used for Artemia production and nutrient enhancement. Table and Figures | Reference | Related Articles | Metrics

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The community structure and biodiversity of nekton in Xiamen sea area, Fujian Province ZHU Jianxin Journal of Fisheries Research    2024, 46 (1): 69-84.   DOI: 10.14012/j.cnki.fjsc.2024.01.008 Abstract （163）   HTML （10）    PDF （2358KB）（596）       Knowledge map    Save In light of the beam trawl survey conducted in the Xiamen sea area during April (spring) and November (autumn) of 2021,this study delves into an analysis of the community structure and biodiversity of nekton within this marine region.The findings unveiled a diverse array,encompassing 176 nekton species,categorized into 18 orders,71 families,and 124 genera.This encompassed 107 fish species,18 shrimp species,35 crab species,8 Stomatopoda species,and 8 Cephalopoda species.Regarding the quantitative aspects,the recorded biomass and abundance of nekton were determined as 281.40 kg·km-2 and 14.49× 103 ind·km-2,respectively.Notably,marked spatial and temporal variations were observed across the surveyed stations.Specifically,the resource density exhibited notable disparities,with the eastern waters and Tong’an Bay of the Xiamen sea area displaying relatively higher concentrations.Moreover,the resource density in autumn significantly surpassed that in spring.The relative importance index (IRI) highlighted distinct dominant species across the seasons.Parapenaeopsis hardwickii,Parapenaeopsis cultrirostris,and Chiloscyllium plagiosum were dominant during spring,whereas Johnius belengerii and Leiognathus brevirostris took precedence in autumn.These dominant species primarily comprised groups characterized by small individuals,abbreviated life cycles,and comparatively lower nutritional levels.Analysis of nekton indices revealed substantial variability in richness,with relatively significant fluctuations.Conversely,diversity and evenness indices displayed minor alterations.Notably,spring exhibited significantly lower values than autumn,positioning the biodiversity at a moderate level.Furthermore,noteworthy interdecadal alterations in the nekton community structure were evident.Through these observations,this study contributes valuable insights into the dynamic composition,seasonal variations,and structural changes within the nekton community in the Xiamen sea area,offering a comprehensive understanding of its biodiversity and ecological dynamics. Table and Figures | Reference | Related Articles | Metrics

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Study of AVS-SEM in surface sediments in cage farming of Zhanghu reservoir bay, Fujian Province JIANG Yixiong, JIANG Dongsheng, CHEN Zhi, ZOU Lizhen, CUI Lifeng, LIAN Chenyang Journal of Fisheries Research    2023, 45 (6): 559-568.   DOI: 10.14012/j.cnki.fjsc.2023.06.006 Abstract （159）   HTML （4）    PDF （1476KB）（140）       Knowledge map    Save In order to investigate the potential ecological risks of heavy metals at cage farming in surface sediments, 5 samples were collected from Zhanghu reservoir bay in Shuikou reservoir and the acid volatile sulfide (AVS) and simultaneously extracted metals (SEM) were studied. The results indicated that the AVS concentrations ranged from 0.57-2.13 μmol·g-1, and the SEM concentrations ranged from 1.66-6.35 μmol·g-1. Among heavy metals, Zn, Cr and Cu were the dominating metals, accounting for over 80% of SEM. By comparing the ratio of SEM to AVS, the values of annual average were ranged from 1.65-9.11. If Cr was not included in the calculation, the values were ranged from 1.42-7.75, and the value of (SEM-AVS) were mainly smaller than 5. These indicated that heavy metals were associated with the AVS phase in the sediments, while metals in surface sediments could not cause toxicity to benthic organism. Compared with the threshold of toxic effects, the most toxic metal was Pb in terms of individual heavy metals. According to the present study, the potential toxic effects of heavy metal release and hydrogen sulfide (H2S) production from sulfides on cage aquaculture should be on the alert. Table and Figures | Reference | Related Articles | Metrics

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Preparation and modification of biochar and its application in the treatment of aquaculture wastewater ZENG Cheng, ZHENG Huidong, XU Yibin, LIN Qi Journal of Fisheries Research    2024, 46 (2): 198-206.   DOI: 10.14012/j.cnki.fjsc.2024.02.011 Abstract （148）   HTML （4）    PDF （1320KB）（305）       Knowledge map    Save Biochar has gained significant attention in the field of sewage treatment, both domestically and internationally, due to its favorable adsorption capabilities and low cost. While ordinary biochar does exhibit certain adsorption effects, its performance is less effective when dealing with high concentrations or specific pollutants. However, the performance of modified biochar in sewage treatment shows significant improvement compared to unmodified biochar. Modification processes result in a larger specific surface area, larger pores, and a higher concentration of functional groups. These modifications not only enhance its adsorption capability, but also enable the specific adsorption of organic pollutants, making it highly valuable in the treatment of aquaculture wastewater. This paper provides a review of the recent research status of biochar at both domestic and international levels, highlighting several common methods of biochar preparation and modification, as well as discussing their respective advantages and disadvantages. The mechanisms of action of biochar and its application in the treatment of aquaculture wastewater are explained, while also exploring the factors that have hindered the development and adoption of biochar. Countermeasures and suggestions are proposed, along with a prospect of its application prospects in the field. Table and Figures | Reference | Related Articles | Metrics

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Potential effects of microplastic contamination on muscle tissue metabolism of Katsuwonus pelamis in the Eastern Pacific Ocean WU Lijun, PAN Zhong, LI Weiwen, DAI Xiaojie, HE Weiyi, HUANG Hao, OU Danyun, WANG Lei Journal of Fisheries Research    2024, 46 (1): 49-59.   DOI: 10.14012/j.cnki.fjsc.2024.01.006 Abstract （147）   HTML （5）    PDF （1163KB）（209）       Knowledge map    Save Microplastic pollution is widespread worldwide and can enter organisms by ingestion or inhalation.In this study,an experimental group (with microplastics) and a control group (no microplastics detected) were set up,and a non-targeted metabolomic analysis was performed on K.pelamis muscle tissue from the Eastern Pacific Ocean using liquid chromatography-mass spectrometry to investigate the potential effects of microplastic contamination on their metabolites.The results showed that a total of 34 metabolites were significantly different,of which 19 differential metabolites were up-regulated and 15 differential metabolites were down-regulated.The differential metabolite analysis showed that methylmalonic acid and propionic acid were up-regulated to induce neurological disorders and may be responsible for the abnormal swimming pattern of K.pelamis.The metabolite content of 20-hydroxyarachidonic acid in the metabolites of K.pelamis muscle tissue was significantly upregulated (P=0.003) and the difference multiplier was 6.002-folds.Arachidonic acid metabolism was closely related to hepatic disorders of glycolipid metabolism and its abnormal metabolism may affect disorders of glycolipid metabolism.Malic acid downregulation might put K.pelamis at risk of bacterial infection.In contrast,glycerol-3-phosphate,creatine phosphate and hydroxy cumene glycosides were up-regulated as a self-protective mechanism for K.pelamis in response to microplastic accumulation.Metabolic pathway enrichment analysis showed that different metabolites were mainly enriched in metabolism,bile secretion,propionate metabolism,proximal tubular bicarbonate recycling,ethylbenzene degradation,glycerophospholipid metabolism,and cholesterol metabolism.Among them,cholesterol metabolism and glycerophospholipid metabolism were involved in building biofilms and maintaining the basic life activities of the organism;while ethylbenzene degradation and propionate metabolism were used to alleviate toxicity in vivo;and proximal tubular bicarbonate recycling was responsible for mediating blood pH.The results of the study provided some reference significance for the toxicology of microplastic pollution on marine fish. Table and Figures | Reference | Related Articles | Metrics

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Study on the effects of Bacillus subtili on the gut microbiota of different fish varieties and water quality as water additive ZHANG Jingjing, LIN Yu, CHEN Duhuang, CHEN Bin, XUE Lingzhan, FAN Haiping Journal of Fisheries Research    2024, 46 (2): 136-146.   DOI: 10.14012/j.cnki.fjsc.2024.02.004 Abstract （146）   HTML （12）    PDF （2329KB）（242）       Knowledge map    Save To investigate the effects of Bacillus subtilis as water additive on gut microbiota in Spinibarbus caldwelli, Acrossheilus hemispinus and Hypophthalmichthys molitrix and its purification effect on aquaculture water, the gut microflora in the foregut, midgut and hindgut of three fishes were investigated through 16S rDNA sequencing and the pH, the levels of ammonia nitrogen and nitrite in the aquatic water were measured. The results suggested B.subtilis could reduce the contents of ammonia nitrogen by 46.39% in water stably since the 11th day and nitrite by 84.61% in water stably since the 19th day. The gut microflora in each intestinal site between experiment and control group were significantly different overall and the abundance of intestinal flora decreased in the experiment group. On the whole,in experiment group, the abundance of intestinal microbiota decreased and the phylum abundance of Actinobacteria and Planctomycetes increased and decreased, respectively. The genus levels of Bacillus increased while pathogenic bacteria Aeromonas and Vibrio decreased. The KEGG signal pathways showed that the metabolic functions of the H.molitrix were enhanced. This study showed that B.subtilis could reduce the level of ammonia nitrogen and nitritein in the aquatic water and purify the aquatic wate, while inhibiting the propagation of harmful intestinal microbiota and improving the metabolic level of intestinal microbiota in H.molitrix. Table and Figures | Reference | Related Articles | Metrics

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Research progress of paralytic shellfish toxins in aquatic products NIU Yaolu, SU Jie Journal of Fisheries Research    2024, 46 (2): 207-214.   DOI: 10.14012/j.cnki.fjsc.2024.02.012 Abstract （143）   HTML （7）    PDF （923KB）（222）       Knowledge map    Save In recent years, harmful algal blooms have frequently occurred in coastal waters of China. Among the microcystins produced by these blooms, paralytic shellfish toxins (PST) are the most widely distributed and harmful, leading to significant concerns. When marine organisms ingest PST, they are passed up the food chain and accumulate, eventually posing a serious threat to human health, the environment, and the ecosystem.Therefore, this paper primarily introduces the definition, distribution, harm, toxicity mechanism, enrichment, and transformation of PST. It also discusses the methods used to detect PST, including biological, chemical, and biochemical approaches. The paper expounds on research trends and regulatory recommendations for PST. Its aim is to serve as a reference for the improvement of research, detection, and prevention of PST. Reference | Related Articles | Metrics

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Nutritional analysis and comprehensive evaluation of by-products of sea cucumber( Apostichopus japonicus) WANG Yangduo, SU Yongchang, WANG Xiaoyan, SHI Wenzheng, LIU Zhiyu Journal of Fisheries Research    2023, 45 (6): 577-584.   DOI: 10.14012/j.cnki.fjsc.2023.06.008 Abstract （141）   HTML （5）    PDF （1080KB）（125）       Knowledge map    Save This article focused on the typical processing by-products of sea cucumber (A.japonicus), including intestines and ovum, to explore their nutritional composition and conduct a comprehensive evaluation by comparing them with body walls. The results showed that the crude protein, crude fat, ash, and total sugar contents in sea cucumber intestines and ovum were 68.85%, 13.53%, 9.96%, 0%,and 58.18%, 12.24%, 19.01%, 2.36%, respectively. In comparison to the body walls, the by-products exhibited higher crude protein and fat contents, but lower ash and total sugar contents. The intestines and ovum were found to contain 18 different amino acids, constituting 68.15% and 51.38% of the total amount, respectively. The ratio of essential to non-essential amino acids was 65.17% and 61.37%, respectively, with essential amino acids accounting for 39.46% and 38.03% of the total amino acids. The essential amino acid index was 94.85 and 67.73, respectively, suggesting that both the intestines and ovum possessed high nutritional value as they exhibited a good balance effect of amino acid components, identifying them as ideal protein sources. The unsaturated fatty acids constituted a similar proportion of total fatty acids, with 80.82% and 80.74%, respectively. Notably, EPA and DHA were the major components of these fatty acids. Additionally, five macro elements and five trace elements were detected, with sodium and iron being the primary elements. This study serves as a foundation for the high-value utilization of sea cucumber by-products and the development of active substances. Table and Figures | Reference | Related Articles | Metrics

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Preliminary study on resource distribution and resource density of sea cucumbers in Pingtan sea area of Fujian Province CAI Jiandi, WU Jianshao, MA Chao, FAN Yilong, LIU Yong, XU Chunyan, LIN Qi, YANG Qiuhua Journal of Fisheries Research    2023, 45 (6): 544-550.   DOI: 10.14012/j.cnki.fjsc.2023.06.004 Abstract （141）   HTML （6）    PDF （2098KB）（173）       Knowledge map    Save This study was performed to explore the distribution and resources of natural sea cucumbers in the inshore aquaculture area in Pingtan District (Fujian Province, China). The study conducted surveys to collect information on species, biology, resource amount, fishing amount, and fishing output value of sea cucumbers in the coastal aquaculture area of Pingtan in April, May, and June 2023. Moreover, the samples of sea cucumbers (n=242) were sampled and used for classification. The results showed that all samples were identified as Apostichopus japonicus. Biological measurement results showed that sea cucumbers collected in April, May, and June reveal average body lengths of (131.7±44.3), (150.1±40.6) and (114.9±28.3) mm, average body weights of (52.2±41.6), (108.3±55.9), (56.1±32.2) g, feeding levels of 3~4, 1~4, and 0~3, respectively. Furthermore, the questionnaire survey results of six fishing boats showed that the average sea cucumber catch per boat in April, May, and June was 1 050.0, 683.3, and 233.3 kg, respectively.This is the first report on the possible existence of a self-propagating population of sea cucumber in Pingtan sea area,which provides information on the resources of sea cucumber in Fujian Province. Table and Figures | Reference | Related Articles | Metrics

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Research progress of biofouling prevention technology for marine aquaculture cages WU Yewei, SHI Jiangao, ZHANG Wenyang, LÜ Changlin, ZHAO Liang, CAO Yiru Journal of Fisheries Research    2024, 46 (3): 228-235.   DOI: 10.14012/j.jfr.2023087 Abstract （140）   HTML （8）    PDF （543KB）（255）       Knowledge map    Save When the biofouling on the marine aquaculture cage are too much, it will affect the exchange volume of the internal and external water bodies in the cage, shorten the service life of the netting, and even endanger the health of the aquaculture organisms. How to solve the attachment of biofouling has become a difficult problem in developing marine aquaculture cages. This paper briefly describes the current situation of antifouling technology of marine aquaculture cages at home and abroad, focuses on the research progress of antifouling technology of marine aquaculture cages, such as mechanical cleaning, coating antifouling method, metal alloy netting antifouling method, points out the problems faced by the current antifouling technology of marine aquaculture cages and gives suggestions. The aim of this paper is to provide reference for the antifouling biotechnology of marine aquaculture cages and the sustainable health development of mariculture industry. Reference | Related Articles | Metrics

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Influence of exogenous additives on the gelatinization characteristics and nutritional quality of bay scallop mince( Argopecten irradians) HUANG Sijia, SUN Shiwen, LI Meng Journal of Fisheries Research    2024, 46 (2): 111-120.   DOI: 10.14012/j.cnki.fjsc.2024.02.001 Abstract （137）   HTML （28）    PDF （1393KB）（203）       Knowledge map    Save In order to develop high quality scallop mince products, this paper took frozen Argopecten irradians scallops as the raw material, and selects soybean isolate protein, hydroxypropyl cassava starch, carrageenan, and egg albumen to be added to the A.irradians mince, which explored the effects of exogenous additives on the gelatinization characteristics and nutritional quality of A.irradians mince.The results showed that 5% soybean isolate protein (Group B) and hydroxypropyl cassava starch (Group C) were effective in improving the textural properties when added to A.irradians mince respectively, and the latter was able to significantly improve the water-holding capacity of A.irradians mince products, but 8% egg albumen (Group D) or 0.6% carrageenan (Group E) decreased the hardness, cohesiveness, springiness, and water-holding capacity of A.irradians mince. The combination of exogenous additives (Group F: 7% hydroxypropyl tapioca starch, 1.0% carrageenan,Group G: 8% hydroxypropyl cassava starch+1.5% carrageenan+5% egg albumen+4% soybean isolate protein) had a lesser effect on the fabrication structure of the A.irradians mince and was able to improve the water-holding capacity. Group F A.irradians mince products had higher sensory ratings in terms of flavor, color and histomorphology. The A.irradians mince products from groups C, F and G all had superior amino acid and fatty acid ratios, with significant differences in amino acid composition, and significantly higher unsaturated fatty acid contents than saturated fatty acids. The content of EPA+DHA accounted for 45.04%, 44.74% and 30.87% of the total fatty acids, separately. It could be seen that the type and proportion of exogenous additives significantly affect the gelatinization behavior and nutritional quality of minced scallop meat in A.irradians, providing theoretical data for the subsequent development of scallop mince products with different textural structures and cost levels. Table and Figures | Reference | Related Articles | Metrics

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Effects of dietary lipid levels on growth performance, body composition, biochemical indices and liver enzyme activities of juvenile Epinephelus fuscoguttatus ♀ × E. polyphekadion ♂ XIE Ruitao, LIU Min, WANG Zhuoduo, HUANG Jiansheng, CHEN Gang, WU Xugan, ZHANG Haitao Journal of Fisheries Research    2024, 46 (4): 311-321.   DOI: 10.14012/j.jfr.2024004 Abstract （136）   HTML （12）    PDF （777KB）（114）       Knowledge map    Save Objective: The study aimed to investigate the impact of dietary lipid levels on the growth performance, body composition, and biochemical indices of juvenile Epinephelus fuscoguttatus ♀ × E. polyphekadion ♂ hybrid (approximately 7.16 g). Methods: An 8-week feeding trial was conducted, during which five isonitrogenous experimental diets containing 5.86%, 7.74%, 9.72%, 11.74%, and 13.68% dietary lipid (referred to as L1, L2, L3, L4, and L5, respectively) were formulated using fish oil as the primary lipid source. Results: 1) The optimal growth performance of juvenile E. fuscoguttatus ♀ × E. polyphekadion ♂ was observed at a dietary lipid level of 9.72%?11.74%. At this level, weight gain rate (WGR) and condition factor (CF) significantly increased (P<0.05), while feed conversion ratio (FCR) decreased significantly (P<0.05). 2) Dietary lipid levels did not significantly affect whole body and muscle moisture content (P>0.05). However, increasing dietary lipid levels led to a gradual increase in crude lipid content in both whole body and muscle, accompanied by a decrease in crude protein content in the whole body. 3) As dietary lipid levels increased, the levels of serum total protein (TP), albumin (ALB), and low-density lipoprotein (LDL) decreased, while serum glucose (GLU), triglycerides (TG), total cholesterol (TC), high-density lipoprotein (HDL), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) activities increased. 4) Liver superoxide dismutase (SOD), catalase (CAT), and lysozyme (LZM) activities increased gradually with increasing dietary lipid levels up to 9.72%. However, CAT levels decreased when dietary lipid levels exceeded 9.72%, and SOD levels decreased when dietary lipid levels exceeded 11.74%. 5) Second-order polynomial regression analysis of WGR and FCR indicated that the optimal dietary lipid level for juvenile E. fuscoguttatus ♀ × E. polyphekadion ♂ were 9.77% and 10.39%, respectively. Conclusion: Under the conditions of this practical test, the recommended amount of fat in the diet of juvenile E. fuscoguttatus ♀ × E. polyphekadion ♂ was 9.77% ? 10.39%. Significance: These results will be beneficial for the development of precise and efficient compound feed for juvenile E. fuscoguttatus ♀ × E. polyphekadion ♂. Table and Figures | Reference | Related Articles | Metrics

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Effect of chitosan oligosaccharides on non-specific immunity of Eriocheir sinensis GAO Yujia, XU Mei, XU Yunshu, XU Tiantian, ZHANG Minna, XU Qingsong Journal of Fisheries Research    2023, 45 (6): 530-536.   DOI: 10.14012/j.cnki.fjsc.2023.06.002 Abstract （133）   HTML （4）    PDF （1838KB）（127）       Knowledge map    Save This study aimed to investigate the effect and mechanism of immune enhancer chitosan oligosaccharides (COS) on non-specific immunity of E.sinensis. A total of 45 crabs (20±2) g with intact appendages and uniform individuals were selected for the experiments, and they were randomly divided into three groups, with fifteen crabs in each group. Each crab was injected with 0.1 mL of COS at concentrations of 0 (control group), 50 and 200 μg·mL-1, respectively. Serum and blood lymphocytes were collected 24 hours later to analyze the changes in immune enzyme activity and immune genes, etc. The results showed that compared with the control group, COS treatment significantly increased the activities of superoxide dismutase, catalase, glutathione peroxidase and lysozyme in the serum of crab, significantly increased the content of nitric oxide, and decreased the content of malondialdehyde in serum. Quantitative real-time PCR results showed that compared with the control group, COS significantly increased the expression of EsToll1, EsToll2, EsMyD88, EsTube, EsPelle and EsDorsal in the Toll signaling pathway, and up-regulated the expression of anti-lipopolysaccharide factors EsALF and EsALF3. These results suggested that COS could improve the non-specific immunity and antioxidant capacity of E.sinensis, activate the Toll signaling pathway, and promote the expression of anti-lipopolysaccharide factors. This study provided theoretical support for the application of COS in E.sinensis. Table and Figures | Reference | Related Articles | Metrics

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Discussion on establishing a service system for Fujian deeper offshore aquaculture industry WANG Xiaowei, CHEN Xifei, LI Miaomiao, KANG Jianping Journal of Fisheries Research    2023, 45 (6): 614-620.   DOI: 10.14012/j.cnki.fjsc.2023.06.012 Abstract （130）   HTML （8）    PDF （1044KB）（214）       Knowledge map    Save Deeper offshore aquaculture plays a crucial role in mitigating the ecological pressures in nearshore waters, expanding the aquaculture industry’s development space, and offering high-quality green aquatic products. In Fujian Province, the deeper offshore aquaculture is still in its early stages, and it has introduced the “Fujian Province Pilot Work Plan (2021—2023) For Deepsea Equipment Breeding Project” to research and tackle the technological challenges related to this industry. The plan aims to establish a complete industry chain, enhance the industrial service system, and explore novel fields, mechanisms, and models for deeper offshore aquaculture. This paper is centered around the developmental requirements of the deep-sea aquaculture industry and follows a problem-oriented approach. It presents an industry development service system for deeper offshore aquaculture that consists of three main links and six systems,including infrastructure construction services and financial support services during the pre-production stage, production technology services and industrial supply chain services during the mid-production stage, and brand building services and processing and circulation services during the post-production stage. Moreover, it provides strategies and suggestions for constructing a service system, aiming to establish a comprehensive support system that enables the sustainable development of the deep-sea aquaculture industry. Table and Figures | Reference | Related Articles | Metrics

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Research progress of isothermal amplification technique applied to the detection of Enterocytozoon hepatopenaei TAN Nianqiu, WEI Chunmei, WEN Yusong, FAN Xiaodong Journal of Fisheries Research    2024, 46 (1): 101-110.   DOI: 10.14012/j.cnki.fjsc.2024.01.011 Abstract （127）   HTML （6）    PDF （941KB）（232）       Knowledge map    Save Enterocytozoon hepatopenaei (EHP) is a specialized intracellular parasite that has caused huge economic losses in shrimp aquaculture due to its large outbreaks,and no specific drugs have been found to effectively treat EHP infection.Histopathology and molecular biology are the main methods used to detect EHP.The isothermal amplification method,a key technique in molecular biology,has gained significant attention as a potential field detection method owing to its simplicity,cost-effectiveness,high sensitivity,and specificity.This paper provides a comprehensive review of conventional detection methods for EHP,including loop-mediated isothermal amplification (LAMP) and recombinase isothermal amplification (RPA),and discusses their applications in EHP detection.The aim is to offer valuable insights for the early detection,efficient prevention,and control of EHP infection,ultimately promoting the sustainable development of shrimp aquaculture. Table and Figures | Reference | Related Articles | Metrics

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Investigation and analysis of water quality and environment of shellfish culture area of Laizhou Bay, Shandong Province WANG Xuming, LIU Chenglei, NIE Yi, ZHANG Haodong, LIU Jiazhuo, WU Xuran, LIU Xiaoling Journal of Fisheries Research    2024, 46 (3): 286-295.   DOI: 10.14012/j.jfr.2023108 Abstract （125）   HTML （7）    PDF （1427KB）（135）       Knowledge map    Save By investigating various environmental factors and phytoplankton in specific marine areas, this study serves as a reference to determine whether the lower water environment can support the growth of shellfish, based on traditional upper shellfish culture practices. In spring (end of March) and autumn (end of September) 2022, relevant tests were conducted on seawater in the upper (2 m) and lower (8 m) layers of shellfish culture areas in Longkou City (K1, K2) and Laizhou City (L1, L2) in the eastern part of Laizhou Bay, Shandong Province. The results indicated that water temperature, pH, salinity, dissolved oxygen (DO), chemical oxygen demand (COD), and labile phosphorus (DIP) in both upper and lower layers of the four culture areas met the national second-class water quality standards. Nitrite, nitrate, and ammonia nitrogen (NH4-N) concentrations were within safe limits. N/P ratio analysis revealed phosphorus limitation in both upper and lower layers, except for suitable nitrogen-to-phosphorus ratios under L1 in spring and K1 in autumn. Phytoplankton density was higher in the upper layer than the lower layer spatially, higher in spring than autumn, with lower shellfish food availability in Longkou City during autumn. Longkou City exhibited rich phytoplankton in both upper and lower layers during spring, as well as in both seasons of Laizhou City. Considering cultural literature and the indicators in this paper, it is suggested that the water quality and phytoplankton density in the upper and lower layers of Longkou City and Laizhou City during spring and autumn may provide opportunities for expanding the water layer in shellfish culture. However, the higher total phosphorus content in the lower layer of Longkou City raises concerns about exacerbating eutrophication, coupled with relatively low phytoplankton density in autumn, which may not be conducive to shellfish growth. This study, through water quality investigation, aims to offer data support for dynamically adjusting and optimizing the rational layout of shellfish culture. Table and Figures | Reference | Related Articles | Metrics

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The effect of carotenoids on the body color of Neocaridina denticulata sinensis ZHANG Yuanchang Journal of Fisheries Research    2024, 46 (1): 38-48.   DOI: 10.14012/j.cnki.fjsc.2024.01.005 Abstract （123）   HTML （11）    PDF （2645KB）（170）       Knowledge map    Save To study the effect of carotenoids on the body color of red,yellow and blue strains of N.denticulata sinensis,this study used TLC (thin-layer chromatography) to analyze the main pigment compositions of 3 strains of N.denticulata sinensis.According to the results,the effects of carotenoids on the body color of 3 strains of N.denticulata sinensis were tested.The TLC analysis was used to separate the dermis and extract the pigment of 3 strains of N.denticulata sinensis.The feeding trials were conducted in light and dark,while 300 shrimps from each strain were divided into thirty groups,with three replicates in each group.The control group was fed with basic feed,while the other four groups were fed with basic feed added Spirulina,lutein,natural-astaxanthin and artificial-astaxanthin.The results showed that the pigment substances of red,yellow,and blue strains of N.denticulata sinensis contained β-carotene and astaxanthin;the pigment substances of blue N.denticulata sinensis contained a combination of crustacyanin and astaxanthin.The feeding trials showed that in yellow strain and blue strain,natural-astaxanthin had the most obvious effect on the body color promotion.Under the dark condition,lutein,natural-astaxanthin and artificial-astaxanthin had certain promotion and maintenance effects on the body color of red strain and yellow strain.The four carotenoid additives had no effect on the body color in blue strain.This study provided a theoretical basis for improving body color of N.denticulata sinensis. Table and Figures | Reference | Related Articles | Metrics

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Development of microsatellite markers in Macrobrachium nipponense based on sequencing genotyping LIU Kai, XIE Nan, WANG Yuxi, LIU Xinyi Journal of Fisheries Research    2023, 45 (6): 537-543.   DOI: 10.14012/j.cnki.fjsc.2023.06.003 Abstract （123）   HTML （5）    PDF （1120KB）（119）       Knowledge map    Save The microsatellite sequences were obtained using a simple sequence repeats (SSR) development method without needing a reference genome and were genotyped through sequencing. The ebwt2InDel software was utilized to identify Insertion/Deletion (InDel)-containing sequences from the whole genome sequencing data of two samples. Using MISA software, 111 perfect SSR-containing sequences were obtained from the InDel-containing sequences by eliminating non-SSR-containing sequences. The SSRgenotyper software was then used to analyze the polymorphism of 30 M.nipponrnse samples, successfully genotyping 19 out of the 111 SSR-containing sequences with a genotyping success rate of 17.12%. Successful genotyping was achieved at 19 SSR loci, all exhibiting 3-base repeats ranging from 4 to 6. Among them, the highest proportion of repetitive sequences was observed with the ATC/ATG unit, accounting for six loci (31.58%). Two of the 19 SSR markers had low polymorphism information content, and 17 had moderate polymorphism information content. These 19 microsatellite markers helped to evaluate the genetic parameters of freshwater shrimp populations and develop freshwater shrimp germplasm resources. Table and Figures | Reference | Related Articles | Metrics

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Development status and prospect of gravity deep water cage in China XUE Zun, SHI Jiangao, ZHANG Wenyang Journal of Fisheries Research    2024, 46 (4): 393-402.   DOI: 10.14012/j.jfr.2024011 Abstract （122）   HTML （7）    PDF （2590KB）（204）       Knowledge map    Save Background: Due to the depletion of global marine fishery resources and the increasing pressure of fishing, traditional cage culture is limited by space and cannot meet the urgent needs of human beings for seafood. Gravity deep water cage culture can make better use of marine fishery resources, which is one of the important ways to solve this problem. Progress: Therefore, this paper analyzes the development of gravity deep-water cage in China, describes the research direction and application of gravity deep-water cage, and prospects its future. So far, China’s gravity deep-water cage technology has made great progress, and some achievements such as rope mesh materials, aquaculture supporting equipment and aquaculture technology have been industrialized. [Outlook] At the same time, China’s gravity deep water cages need to continue to innovate and improve in terms of cage structure and materials, intelligent management and green aquaculture technology, in order to promote the high-quality development of deep water cages. Significance: The development of China’s gravity deep-water cage not only helps to alleviate the pressure of offshore cage farming, but also promotes the transformation and upgrading of China’s marine cage farming industry, and contributes to the prosperity of China’s aquaculture industry and the protection of the marine ecological environment. Table and Figures | Reference | Related Articles | Metrics

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The study of the oxygen consumption rate and asphyxiation point of juvenile Eleutheronema tetradactylum under different temperatures LIN Xin, XIE Xiyao, OU Youjun, LI Jia’er, WU Shuiqing Journal of Fisheries Research    2024, 46 (2): 121-128.   DOI: 10.14012/j.cnki.fjsc.2024.02.002 Abstract （121）   HTML （9）    PDF （1097KB）（142）       Knowledge map    Save To investigate the changes in oxygen consumption rate and suffocation point of Eleutheronema tetradactylum under different water temperature conditions, the experiment compared the effects of different temperatures (22,27,32 ℃) on the oxygen consumption of E.etradactylum with an average length of (4.5 ± 0.5) cm and an average body mass of (1.5 ± 0.5) g.The experimental results show that: 1) Under the conditions of this experiment, the higher the water temperature, the shorter the time for all juvenile fish to die, and the higher the mass concentration of dissolved oxygen (DO) in the water. The suffocation point at 22 ℃ is 1.39 mg/L, the suffocation point at 27 ℃ is 1.54 mg/L, and the suffocation point at 32 ℃ is 1.76 mg/L; 2) The oxygen consumption rate of juvenile fish in each temperature group showed a decreasing trend with the extension of experimental time, and the higher the temperature, the faster the decrease rate of oxygen consumption rate. When the oxygen consumption rate decreased from 1.418, 2.346, and 4.243 mg/(g·h) at the beginning of the experiment to 0.4-0.5 mg/(g·h), all juvenile fish died, and the changes in different temperature groups slowed down. The concentration difference between adjacent observation points was not significant (P>0.05), while the survival rate of juvenile fish in the control group was 100%; 3) During the experiment, when the DO content of each temperature group was 2.53, 2.90, and 3.10 mg/L, the juvenile fish began to exhibit disorderly movement, restlessness, occasional jumping out of the water, and gill opening. As the experiment continued, the frequency of the juvenile fish jumping out of the water increased, and the amplitude of gill cover vibration increased accordingly. After intense stress, they lay still at the bottom of the water, twitching and suffocating to death. Table and Figures | Reference | Related Articles | Metrics

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Effects of salinity on digestive enzyme and non-specific immune enzyme of juvenile Sebastiscus marmoratus JIANG Fei, XU Jiabo, LIN Qi, SHI Yonghai, JIA Chuipan, YAN Yinlong, DENG Pingping, YU Aiqing, ZHANG Zhiwen, YUAN Xincheng Journal of Fisheries Research    2024, 46 (2): 129-135.   DOI: 10.14012/j.cnki.fjsc.2024.02.003 Abstract （120）   HTML （8）    PDF （1703KB）（147）       Knowledge map    Save To investigate the changes in digestive enzyme activities in the intestine and stomach, as well as the activities of non-specific immunoenzymes in the liver of juvenile Sebastiscus marmoratus under salinity stress, and to assess the ability of the juveniles to adapt to sudden changes in salinity, a salinity experiment was conducted. The experiment comprised four groups, representing different salinity gradient levels: 6, 8, 10, and 20 (control). These groups were referred to as S6, S8, S10, and S20, respectively. Results indicated that at the acute stress period of 96 hours, the activities of trypsin, pepsin, amylase, and lipase in S10 and S20 were significantly higher compared to those in S6 and S8 (P<0.05). Conversely, the activities of glutamic-pyruvic transaminase and glutamic-oxaloacetic transaminase were significantly higher in S6 and S8 compared to S10 and S20 (P<0.05). There was no significant difference in the activities of ACP and AKP between S6 and S8 (P>0.05). Additionally, the activities of ACP and AKP increased initially and then decreased over time in S10 and S20. These activities in S10 and S20 peaked at 12 hours, signifying a significant increase compared to other salinity levels. These findings suggest that the optimal salinity for breeding juvenile S.marmoratus ranges from 10 to 20. Table and Figures | Reference | Related Articles | Metrics

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A comparative study on the nutrient and fatty acid composition in muscle of Siniperca scherzeri in upstream and downstream area of Yalu River JIANG Xianghui, WANG Xingbing, KOU Lingxiao, WEI Hongxiang, LIU Yong, ZHANG Tao Journal of Fisheries Research    2023, 45 (6): 569-576.   DOI: 10.14012/j.cnki.fjsc.2023.06.007 Abstract （114）   HTML （3）    PDF （1150KB）（161）       Knowledge map    Save To figure out the different nutritional quality of S.scherzeri muscle from different habitats, this study determined and compared the nutritional compositions, as well as fatty acids composition and content of S.scherzeri. The results showed that there was significantly higher crude fat content in the muscle of S.scherzeri in upstream area than that in the muscle of S.scherzeri in downstream area (P<0.05), without significance in content of moisture, crude protein and ash between these two groups (P>0.05). There are 16 kinds of fatty acids in the muscle of S.scherzeri in upstream area and 17 in downstream area. The saturated fatty acids, monounsaturated fatty acids and polyunsaturated fatty acids occupied 35.886%, 34.184% and 29.922% in the muscle of S.scherzeri in upstream area, occupied 34.913%, 34.895% and 30.181% in the muscle of S.scherzeri in downstream area, respectively. And for the composition of fatty acid, the essential fatty acid occupied 11.735%, the ratio of ω-6/ω-3 was 0.919,EPA and DHA was 11.672% in the muscle of S.scherzeri in upstream area. The essential fatty acid occupied 9.571%, the ratio of ω-6/ω-3 was 0.781,EPA and DHA was 12.939% in the muscle of S.scherzeri in downstream area. In conclusion,the nutritive value of fatty acid in the muscle of S.scherzeri in upstream area was better than that of S.scherzeri in downstream area. Table and Figures | Reference | Related Articles | Metrics

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Identification of fish larvae and juvenile by DNA barcoding in Xuwen Jiaowei sea area,Guangdong Province WANG Sijin, WANG Jinrun, CHEN Yanying, HOU Gang Journal of Fisheries Research    2024, 46 (2): 171-180.   DOI: 10.14012/j.cnki.fjsc.2024.02.008 Abstract （112）   HTML （7）    PDF （1361KB）（175）       Knowledge map    Save In this study, COⅠgene fragments were used as DNA barcodes for the molecular identification in fish larvae and juvenile from Xuwen Jiaowei coral reef sea areas in summer and autumn of 2018—2020, and its applicability to the identification in the early stages of fish was investigated. A total of 199 high quality COⅠsequences of fish larvae and juvenile were obtained, among them, 69 sequences matching to species were identified to the species level, and 110 sequences were identified to the genus, family and order level. In all, 32 taxa of 20 genera in 6 orders and 19 families were successfully identified. Among them, 17 taxa were identified to the species level, 5 taxa to the genus level, 9 taxa to the family level, 1 taxa to the order level, and another 9 taxa were not identified. Gobiidae had the most species and quantity in family level, accounting for 17.07% and 31.16%, respectively, followed by Leiognathidae with 7.31% and 8.04%, respectively. The present study indicated that the proportion of species identification based on DNA barcoding for fish larvae and juvenile in the Xuwen coral reef sea area was relatively low, and the gap of DNA barcode sequences for adult fish species was an important factor. Therefore, it was necessary to develop a reliable DNA barcode database for local adult fish. Combined traditional morphological and molecular method might improve the accuracy in identifying fish larvae and juvenile. Table and Figures | Reference | Related Articles | Metrics

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Effects of Gymnodinium catenatum on the clearance rate of Perna viridis XU Cuiya, YANG Fang, CHEN Xiaohong, CHEN Caizhen Journal of Fisheries Research    2023, 45 (6): 551-558.   DOI: 10.14012/j.cnki.fjsc.2023.06.005 Abstract （110）   HTML （4）    PDF （1508KB）（129）       Knowledge map    Save The present study was conducted to investigate the impact of G.catenatum on the feeding behavior of P.viridis. An experimental group fed with G.catenatum and a control group fed with Skeletonema costatum were established. Short-term (1 hour) and long-term (38 days) clearance rate experiments were carried out. The results showed that the clearance rate of P.viridis, whether feeding on toxic or non-toxic algae, as the densty of algae cells increased, initially increased and then decreased, reaching the peak when the algal cell density approached the threshold density for red tide. The highest clearance rates observed in the experimental and control groups were (3.04±0.02) and (3.70±0.03) L/(g·h), respectively. Compared to the control group, the clearance rate of P.viridis in the experimental group exhibited a significant decrease (P<0.05), indicating that G.catenatum had an influence on the feeding behavior of P.viridis. Furthermore, the long-term filtration did not cause significant changes in the clearance rate, which remained relatively stable at the initial level. Table and Figures | Reference | Related Articles | Metrics

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Investigation on fish resources of Spinibarbu scaldwelli National Aquatic Germplasm Resources Reserve in Huyangxi River, Yongchun County, Fujian Province in winter GUO Shaopeng, FANG Minjie, HUANG Zhisen, ZENG Qingmin, WEN Ping, LI Leibin, LIU Jixiang Journal of Fisheries Research    2024, 46 (3): 279-285.   DOI: 10.14012/j.jfr.2023120 Abstract （107）   HTML （6）    PDF （727KB）（102）       Knowledge map    Save To investigate the status of fish resources within the Spinibarbu scaldwelli National Aquatic Germplasm Resources Reserve in Huyangxi River, Yongchun County, Fujian Province (referred to as the reserve), and assess the proliferation and release effects of in recent years, fishing methods such as gill nets and ground cage nets were employed to survey fish resources in the reserve in January, 2023. The survey revealed 28 fish species in the reserve during winter, comprising 3 orders, 7 families, and 25 genera. Within these, 16 genera and 18 species belonged to the Cypriniformes order, constituting 64.28% of total fish species. The order Siluriformes comprised 3 genera and 5 species, accounting for 17.86% of all species, while the Perciformes order consisted of 5 genera and 5 species, also representing 17.86% of all species. From January 7 to January 10, 2023, a total of 1 043 fish weighing 70 222.7 g were captured in the reserve. Dominant species included Coptodon zillii, Sarcocheilichthys nigripinnis, Abbottina elongata, and Distoechodon tumirostris. Among the captured fish, the proportion of S. caldwelli was relatively small, comprising 33 individuals weighted 6 438.0 g, accounting for 3.16% of the total catch by number and 9.27% by weight. Furthermore, the richness index, evenness index, and species diversity index of the reserve in winter were 2.770, 0.723, and 3.513, respectively. The proportion of alien fishes was relatively high, with main species including C. zillii, Oreochromis niloticus, and Sarotherodon galilaeus, indicating a significant invasion of alien fishes in lakes, oceans, and streams. Given that excessive reproduction of alien fishes may impede the survival and reproduction of local fish species, it is imperative to strengthen control measures against alien fishes and protect resources of S. caldwelli and other indigenous fish species. Table and Figures | Reference | Related Articles | Metrics

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Estimation of heritability of growth traits in the juvenile fish stages of Takifugu rubripes PAN Hanbai, YU Hailong, TIAN Yi, CAO Lirong, MA Wenchao, XU Mingjie, WANG Yusen, WANG Peiyang, XIANG Xuejian, QIU Xuemei, BAO Yulong, LIU Shengcong, WANG Xiuli Journal of Fisheries Research    2024, 46 (1): 19-28.   DOI: 10.14012/j.cnki.fjsc.2024.01.003 Abstract （101）   HTML （6）    PDF （1033KB）（165）       Knowledge map    Save In order to obtain the heritability of growth traits in juvenile T.rubripes,and to exert the important role of heritability in guiding breeding work,the body mass,body length and total length of 2 900 juvenile T.rubripes at 1,2 and 4 months old were measured using the whole sib family of T.rubripes constructed in 2022.The heritability of these three growth traits was estimated by the whole sib correlation method and its formula.The phenotypic correlations among these three growth traits were calculated by Pearson coefficient.The genetic correlation of these three growth traits was calculated by sib covariance method.The results showed that the heritability of the 1-month-old juvenile T.rubripes was 0.54 (body mass heritability),0.39 (body length heritability) and 0.36 (full length heritability),respectively,all of which belonged to the high heritability.The genetic correlation between body length and body mass was 0.889 and the phenotypic correlation was 0.851.The genetic and phenotypic correlations of body mass were 0.873 and 0.864,respectively.The genetic correlation between body length and total length was 0.947 and the phenotypic correlation was 0.894.Heritability of body mass,body length and full-length at 2 months old were 0.28,0.23 and 0.25,respectively,all belonging to the medium heritability.The genetic correlation between body length and body mass was 0.828 and phenotypic correlation was 0.917.The genetic correlation and phenotypic correlation were 0.820 and 0.932,respectively.The genetic correlation between body length and total length was 0.897 and the phenotypic correlation was 0.952.At 4 months old,the heritability of body mass,body length and full length were 0.23,0.29 and 0.29,respectively,which belonged to the medium heritability.The genetic correlation between body length and body mass was 0.769,the phenotypic correlation was 0.779,the genetic correlation between body mass and body length was 0.726,and the phenotypic correlation was 0.801.The genetic and phenotypic correlations between body length and total length were 0.912 and 0.941,respectively.The results of this study can provide reference for the subsequent breeding of T.rubripes. Table and Figures | Reference | Related Articles | Metrics

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Effects of environmental factors on the development of Saccharina japonica ‘Dongfang No.7’ gametophyte LI Xia, CHEN Shuxiu, WANG Weiwei, SUN Juan, LI Xiaojie Journal of Fisheries Research    2024, 46 (2): 147-155.   DOI: 10.14012/j.cnki.fjsc.2024.02.005 Abstract （100）   HTML （4）    PDF （3039KB）（148）       Knowledge map    Save The male and female gametophytes of Saccharina japonica ‘Dongfang No.7’ were cultured at different temperatures, photoperiods and nitrogen concentrations to observe the development of gametophytes and explore the optimum environmental conditions for the formation of gametophyte oogonium, ovulation and young sporophyte. The results showed that temperature, photoperiod and nitrogen concentration had significant effects on the development of gametophyte. The rate of gametophyte development at 12 ℃ and 15 ℃ (60%-70%) was significantly higher than that at other temperature groups. At 20 ℃, the gametophyte was in the vegetative growth stage without development. The rate of gametophyte development (80%) in 10L∶14D group was significantly higher than that in other photoperiod groups, followed by that in 6L∶18D group, but the length of young sporophyte in this condition was significantly lower than that in other photoperiod groups. When the concentration of N was 2 mg/L and 4 mg/L, the final development rate was more than 75%, and the sporophyte accounted for about 70%, which was significantly higher than other groups. This study provides theoretical basis and data support for artificial breeding of gametophyte of S.japonica ‘Dongfang No.7’. Table and Figures | Reference | Related Articles | Metrics

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Research progress on immunoglobulin of yolk in the prevention and treatment of aquatic animal diseases ZHENG Jiemin, YOU Jiantao, WU Liang, LIN Jiaming, HE Yibin, XU Bin, XUE Haibo Journal of Fisheries Research    2024, 46 (2): 190-197.   DOI: 10.14012/j.cnki.fjsc.2024.02.010 Abstract （99）   HTML （6）    PDF （1016KB）（287）       Knowledge map    Save Yolk immunoglobulin (IgY), a polyclonal antibody, has garnered considerable attention for its role in conferring passive immunity against bacterial and viral diseases. This paper comprehensively reviews the current research on the fundamental structure and physicochemical properties of IgY. Additionally, the paper emphasize the diverse applications of IgY in preventing and treating bacterial and viral diseases, offering valuable insights for future research and the broader utilization of IgY in aquaculture. Table and Figures | Reference | Related Articles | Metrics

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Analysis of volatile components of different yeast extract and their effects on sensory quality and gel properties of fish sausages REN Zhongyang, ZHAI Yingying, WU Dan, ZHU Xinyi, XIONG Jian, LI Pei, HUANG Qilin Journal of Fisheries Research    2024, 46 (5): 425-435.   DOI: 10.14012/j.jfr.2024054 Abstract （99）   HTML （19）    PDF （751KB）（75）       Knowledge map    Save Objective: In order to investigate the difference of volatile components of different yeast extract (YE) and their effects on the sensory quality and gel properties of fish sausages. Methods: The volatile components of five kinds of YE like KA66, FA28, FIG18LS, KU012 and FA01 were analyzed by headspace solid-phase microextraction combined with gas chromatography-mass spectrometer (GC-MS). Meanwhile, the effects of five YE on sensory quality, whiteness, water holding capacity and gel properties of fish sausages were analyzed by sensory evaluation, colorimeter, hardness meter, and texture analyzer (TPA, puncture). Results: KA66, FA01, FA28, FIG18LS and KU012 were detected with 21, 21, 21, 11 and 24 volatile components, respectively. The contents of volatile components were KA66, FA28 and FA01, KU012 and FIG18LS in descending order. According to the results of odor activity, the key volatile components for KA66 were hexanal, heptanal, phenylacetaldehyde, nonanal, capraldehyde and 1-octyl mercaptan, and for FA28 were heptanal, phenylacetaldehyde, hydrocarbons and capraldehyde. Meanwhile, the key volatile components for FIG18LS were nonanal, and for KU012 were dimethyl trisulfide, nonanal and decanal, and for FA01 were heptanal, phenylacetaldehyde, nonanal, decanal, isovaleraldehyde, 3-methyl-thio-propionaldehyde, octanal and methyl butyrate. However, there was only one same key volatile compound for YE, namely nonanal. The addition of YE could significantly reduce the whiteness of fish sausages, and mainly affect the b* value of fish sausages, especially FIG18LS followed by KU012, due to the YE’s own yellow color. Besides, the water holding capacity of fish sausages treated with KU012 obviously reduced (P<0.05), while KA66 and FA01 had no significant effect on the water holding capacity of fish sausages. Texture analysis showed that the addition of YE had a significant effect on the texture quality of the fish sausages, but had no significant effect on the degree of breakage. The results of texture analysis parameters showed that adding YE could increase the springness, cohesiveness and resilience of fish sausages, but reduce the hardness and chewiness of fish sausages (P<0.05). Among them, KA66 has the best effect on improving the springness of fish intestines, and KU012 has the best effect on improving the cohesiveness and resilience of fish sausages. The results of puncture experiments showed that all yeast extract except FA28 could enhance the breaking strength of fish sausages, and KA66 works best for increasing the breaking distance of fish sausages. Conclusion: The addition of YE can improve the sensory quality of fish intestines, reduce the fishy taste of fish sausages, especially the flavor quality including taste and odor. Therefore, this study could provide the theoretical basis and technical support for YE to improve the quality of silver carp products. Table and Figures | Reference | Related Articles | Metrics

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Effects of high temperature stress on the lethality of fry and adult Glyptosternon maculatum ZENG Rongjun, LIU Xiaorui, GENG Qingbao, TANG Xuemei, FANG Yuanlin, WU Lei, ZHANG Huan, GU Zhengxuan, YU Meng Journal of Fisheries Research    2024, 46 (2): 156-161.   DOI: 10.14012/j.cnki.fjsc.2024.02.006 Abstract （96）   HTML （7）    PDF （892KB）（100）       Knowledge map    Save In order to explore the tolerance of Glyptosternon maculatum to extreme temperatures at different stages, this study performed acute hyperthermic stress on the fry and adult fish, determined semi-lethal time at each temperature, and measured the respiratory rate of adult fish under hyperthermia. The results showed that the semi-lethal time (LT50) of fry G.maculatum shortened with the increase of temperature, and there was a significant difference in the LT50 of the different temperature groups (P<0.05), among which LT50 was 13.53 h at 28 ℃ and 71.20 h at 24 ℃. The respiratory rate of adult fish under high temperature stress increased significantly, and the semi-lethal time (LT50) of adult fish at 24 ℃ was 15.6 h, which was much lower than that of fry at this temperature. The results showed that there were differences in heat tolerance at different growth stages, and attention should be paid to water temperature control during the breeding process. The short-term lethal temperature of fry was 28 ℃, and the short-term lethal temperature of adult fish was 24 ℃. The results of this study can provide basic data for healthy breeding and further study of high temperature tolerance mechanism of G.maculatum. Table and Figures | Reference | Related Articles | Metrics