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    Current situation and development suggestions of aquaculture commercial breeding in China
    HAN Feng, SUN Guangwei, SHI Bo, ZHAO Ming, LIU Ming, ZHENG Yuanyuan, PENG Ruibing
    Journal of Fisheries Research    2022, 44 (4): 387-394.   DOI: 10.14012/i.cnki.fjsc.2022.04.010
    Abstract1463)   HTML2)    PDF (1319KB)(377)       Save

    Aquatic seed industry is the cornerstone of the development of aquaculture.Promoting the construction of commercial seed breeding system can solve the prominent problem of the separation between scientific research and market in the traditional breeding system.In order to further promote the development of aquatic commercial breeding,this paper analyzed the development status and shortcomings of China’s aquatic seed industry enterprises through literature query,data statistics and case comparison.It was considered that the development momentum of China’s aquatic seed industry enterprises was good,which was reflected in the improvement of enterprise breeding awareness,the effective application of seed conservation and control technology,and the diversity of commercial breeding forms.At the same time,China’s aquatic seed industry enterprises also had problems such as weak enterprise competitiveness,development potential and risk resistance,which were reflected in weak independent innovation ability,less investment in breeding R & D,small enterprise scale and so on.In this regard,we proposed to further enhance the competitiveness of China’s aquatic seed industry and promote the commercial development of aquatic breeding by formulating breeding innovation strategies from the two aspects of breeding objects and breeding process,extending vertically and broadening the seed industry chain horizontally.

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    Analysis of the genetic diversity and sequence variation of mitochondrial COⅠgene from three populations of Macrobrachium rosenbergii
    JIANG Fei, DAI Xilin
    Journal of Fisheries Research    2023, 45 (1): 8-13.   DOI: 10.14012/j.cnki.fjsc.2023.01.002
    Abstract660)   HTML3)    PDF (1100KB)(254)       Save

    In order to investigate the genetic diversity among three populations of Macrobrachium rosenbergii collected from Shanghai breeding population (SC), Zhejiang cultured population (ZJ) and Thailand wild population (TL), the mitochondrial sequences of the COⅠ gene in the three populations were amplified and determined by PCR and sequencing. The result showed that 8 nucleotide sites and 4 haplotypes were found in the 458 bp long partial gene segment. The mean content of A+T (54.4%) was higher than G+C (45.7%). Genetic variation parameters showed the population of highest haplotype diversity was TL population (Hd=0.733), the population of highest nucleotide diversity was ZJ population (π=0.005). AMOVA showed that genetic variations among populations (74.5%) was higher than that within populations (25.5%). The tendency of molecular phylogenetic trees which were constructed by NJ and UPMGA method was similar. SC population and ZJ population were the closest genetically, and they clustered into a small branch, then with TL population. These findings were expected to provide reference information for the protection and utilization of germplasm resources of M.rosenbergii.

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    Studying on suitable light intensity,cultivation density and development degree for the gametophyte advance development of Saccharina japonica
    ZHANG Xichang, ZHAO Ningning, LIU Jianbo, LENG Xiaofei, WANG Fasheng
    Journal of Fisheries Research    2022, 44 (4): 345-355.   DOI: 10.14012/i.cnki.fjsc.2022.04.005
    Abstract638)   HTML0)    PDF (2533KB)(99)       Save

    This study sought to investigate the optimum light intensity,cultivation density and the gametophyte advance development degree of Saccharina japonica,in order to provide technical support for gametophyte seedling.The suitable light intensity and cultivation density for the gametophyte advance development were determined by inspecting the female gametophyte development rate,ovulation rate and seminal vesicle formation rate.The suitable degree of gametophyte advance development was determined by inspecting the emergence rate and emergence time.It was found that the advance development speed of gametophyte under 37.5~62.5 μmol·m-2s-1 was significantly faster than that under 25.0 μmol·m-2s-1 in the condition of(13±1)℃ and 10.0 g·L-1.The advance development speed of gametophyte in the density of 5.0~10.0 g·L-1 was significantly faster than that in the density of 15.0~20.0 g·L-1 in the condition of(13±1)℃ and 37.5 μmol·m-2s-1,and only partial gametophyte developed,some gametophyte grew filament in the density of 15.0~20.0 g·L-1.The emergence test was carried out after the gametophyte was advance developed under the conditions of(13±1)℃,37.5 μmol·m-2s-1 and 10.0 g·L-1.The emergence rate were over 96.0% after 5 days of cultivation when the gametophyte was advance developed for 11~12 d,while the emergence rate was 90.7% after 9 days of cultivation when the gametophyte was without advance developed,which was 4 days later than the advance developed gametophyte.As a result,the suitable condition of light intensity and cultivation density were 37.5 μmol·m-2s-1 and 10.0 g·L-1 for gametophyte advance development of S.japonica,while the suitable advance development time was 11~12 d.

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    Artificial domestication and reproduction techniques of Tachypleus tridentatus
    CHEN Zhi, WENG Zhaohong, ZOU Lizhen, ZHOU Honglei, JIANG Yixiong, FANG Chuihong, LI Yuhong, KWAN Kit Yue
    Journal of Fisheries Research    2023, 45 (3): 246-253.   DOI: 10.14012/j.cnki.fjsc.2023.03.004
    Abstract614)   HTML1)    PDF (1500KB)(262)       Save

    To restore the population of the endangered species Tachypleus tridentatus,artificial domestication and breeding research of T.tridentatus was carried out in Luoyuan County,Fujian Province in 2021—2022.Following the observation results of their natural spawning and nursery grounds,the simulated spawning environment was constructed,and the mating pairs were induced to spawn naturally in the artificial environment.The spawning habitat preferences and ecological requirements for T.tridentatus were determined through artificial incubation and seedling cultivation.This paper summarized the artificial domestication and propagation technology of horseshoe crab,and analyzed the significance of artificial domestication and natural propagation of T.tridentatus,providing theoretical basis for species conservation by the protection and restoration of T.tridentatus populations and artificial incubation in Fujian coastal area.

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    Research advanced of breeding methods on microalgae
    ZHOU Linlin, GUO Chentao, ZHONG Chenhui, LIN Qi
    Journal of Fisheries Research    2023, 45 (2): 182-191.   DOI: 10.14012/i.cnki.fjsc.2023.02.011
    Abstract605)   HTML1)    PDF (1104KB)(354)       Save

    Microalgae is a kind of unicellular photosynthetic autotrophic eukaryotic plant, which grows rapidly and processes high photosynthetic efficiency.It can provide a lot of high value products, such as protein, polyunsaturated fatty acids and bioactive compounds which are important sources of health care, aquaculture and biofuels.In order to improve productivity, microalgae breeding technology has been developed to explore the strains which have larger biomass, more lipid, and the ability of stress resistance.The article summarized the advantages and disadvantages of various breeding technologies and research advanced, in order to provide a reference for the development of microalgae breeding.

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    Effects of acute hypoxic stress on Eleutheronema tetradactylum gill organ and liver tissue damage
    LIN Xin, OU Youjun, WEN Jiufu, XU Shuyu, LI Jia'er
    Journal of Fisheries Research    2023, 45 (1): 14-22.   DOI: 10.14012/j.cnki.fjsc.2023.01.003
    Abstract467)   HTML1)    PDF (12122KB)(104)       Save

    To investigate the effects of acute hypoxic stress on the gill organs and liver tissue damage of juvenile Eleutheronema tetradactylum, a 2 h acute hypoxic stress experiment was conducted with 2 mg/L of dissolved oxygen in the water. Then the gills and liver organs of E.tetradactylum were observed using conventional paraffin sections and HE staining as well as electron microscopic techniques. The results displayed contraction of the blood sinuses in the gill filaments of E.tetradactylum under acute hypoxic stress, with dilated ends; longer gill lamellae, larger intervals and swollen and bulging parts of the epithelium. The parietal crypt of mitochondria-rich cells changed from concave to flat and the intracellular mitochondrial morphology changes from oval to round. Liver tissue showed hepatocyte necrosis and fusion of hepatocytes. Hypoxic stress had a large effect on the gill and liver tissues of E.tetradactylum and caused structural damage to the tissues by 2 h.

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    Analysis and development suggestions of Larimichthys crocea industry of China in 2020
    HAN Chengyi, WU Xiongfei, XU Binfu, HUANG Guangliang, LIANG Anwei, CHEN Jia, LIU Jiafu, CHEN Qingrong
    Journal of Fisheries Research    2022, 44 (4): 395-406.   DOI: 10.14012/i.cnki.fjsc.2022.04.011
    Abstract441)   HTML0)    PDF (1541KB)(267)       Save

    During the last decade of the 20th century,the artificial breeding of Larimichthys crocea accomplished its industrialization in China.Since then,the breeding scale and industrial division of the L.crocea industry have been continuously upgraded,forming a perfected industrial chain covering supplying the fishery necessities(fry,feeding stuff,facilities,etc.) and acquisition,trade,processing and export of the marketable fish.The supporting industries such as logistics,catering and tourism have also been gradually formed.In this report,industry experts and practitioners organized by the L.crocea branch of the China Fisheries Association conducted a comprehensive investigation of China’s L.crocea industry status in 2020,then summarized and analyzed it from ten aspects:seedling breeding,farm distribution and output,feeding stuff usage,major epidemic diseases,quality and safety,yields and market,Corona Virus Disease-19 impact,brand culture construction,supporting policies,financial and insurance services,and existing problems;gived countermeasures and suggestions on the industrial development for reference.The survey results showed that there were some problems in the development of L.crocea industry in China,such as low coverage rate of fine varieties,illegal drug use,low level of intensive and deep processing. Suggestions were put forward to promote the healthy and sustainable development of L.crocea industry in China by vigorously developing seed industry projects,standardizing industrial behaviors,increasing the proportion of medium-sized products,improving the processing rate,winning government support and creating industrial culture.

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    Effects of recirculating aquaculture model on water quality and growth performance of Litopenaeus vannamei
    NIU Jiangbo, WU Zhaojun, LI Yasong, KANG Qiuping, XU Bo, GUO Bing, JIANG Liping, SONG Xiangguo, LIANG Qinlang
    Journal of Fisheries Research    2023, 45 (3): 287-296.   DOI: 10.14012/j.cnki.fjsc.2023.03.009
    Abstract391)   HTML1)    PDF (3635KB)(190)       Save

    By analyzing the growth performance and water quality index differences of Litopenaeus vannamei under the recirculating aquaculture mode,the feasibility of the recirculating aquaculture mode in the aquaculture production was explored.The initial body mass of L.vannamei was (2.39±0.20) g.In this study,a recirculating aquaculture group (RAG) and a traditional industrial aquaculture group (IAG) were set up,with 3 replicates in each group.750 individuals of L.vannamei were stocked per cubic meter,and the salinity of the cultured water was 18.The aquaculture period was from July 23 to September 10,2022,lasting for 50 days.The results showed that the average water temperature of RAG was significantly higher than that of IAG (P<0.05),and the dissolved oxygen was 2.45% higher than that of IAG.The ammonia nitrogen of RAG was significantly lower than that of IAG during the culture period (P<0.01),and the nitrite nitrogen was 24.29% lower than that of IAG after the system matured and stabilized (12 days).Although the survival rate of RAG was 2.17% lower than that of IAG,but the final body mass,unit yield,specific growth rate and weight gain rate were all higher than those of IAG.The feeding rate and feed conversion rate of RAG were lower than those of IAG,but did not show significant difference (P>0.05),and the specific growth rate and geometric mean mass of each group showed a significant negative correlation (P<0.05).The comprehensive results showed that the recirculating aquaculture mode could effectively control the key water quality factor indicators at a low level,which played an important role in water resource conservation and environmental protection.In addition,in the practice of aquaculture production,the growth and production of L.vannamei could also be promoted to a certain extent,and this model had strong feasibility.

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    Studies on feeding and growth characteristic of larva and juvenile of Opsariichthys bidens
    LIU Yinhua
    Journal of Fisheries Research    2022, 44 (4): 365-373.   DOI: 10.14012/i.cnki.fjsc.2022.04.007
    Abstract377)   HTML0)    PDF (1043KB)(113)       Save

    The feeding and the growth characteristic of larva and juvenile of Opsariichthys bidens were studied.The results showed that larva and juvenile of O.bidens were breeded under condition of water temperature 26~30℃.36.67% of the larva opened their mouth and started to feed after age of 3 days.The feeding rate of larva and juvenile was 100.00% after 5 days of age,the average rate of larva and juvenile in the full stomach was 99.20% after 6 days of age,and the relationship between feeding amount(y) and body weight(W) was calculated to be y= -0.000 4 W2+ 0.214 0W-0.200 8.The recommended dietary allowance of the larva and juvenile of O.bidens as 45%~50% of the body weight.The regression equation of total length(Lt) with day age(D) was expressed as Lt=-0.008 1D2+ 0.903 1D +4.159 5.The relationship between the body weight(Wt) and day age(D) was calculated to be W t= 0.075 0D2 +2.027 1D -10.689 0,and total length(Lt) and the body weight(Wt) could be expressed to be Wt= 0.001 3 L t 3.609 ? 8 ,b = 3.609 8,which showed that the growth of larva and juvenile of O.bidens belongs to the type of allometric.The results provided more references for the artificial breeding of O.bidens.

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    Research progress in culture modes and applications of Isochrysis galbana
    LI Daogui, CHEN Na, HONG Yuling, LIN Rongzhao, WU Liming, HE Yongjin
    Journal of Fisheries Research    2022, 44 (3): 294-300.   DOI: 10.14012/j.cnki.fjsc.2022.03.012
    Abstract376)   HTML1)    PDF (1317KB)(720)       Save

    Isochrysis galbana is a sustainable and renewable bio-resource and an important source of natural bioactive compounds.It can provide a wide range of high-value products,such as fucoxanthin,high-quality protein,as well as polyunsaturated fatty acids.These nutrients provide essential dietary nutrients for people and are important sources of health care,aquaculture and biofuels.However,the high cost of microalgae culture has been a limiting factor for the development of the industry.In recent years,the research on the low-cost culture modes and large-scale culture technology of I.galbana was also deepening.In this paper,the research progress on microalgae culture modes and large-scale culture of I.galbana was reviewed,and the functional value development of I.galbana in recent years was summarized,while the future application of I.galbana in functional food,aquaculture,biofuel and other fields was prospected.

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    Characteristics,existing problems and countermeasures of diamond shrimp culture( Penaeus monodon)in Zhangzhou City, Fujian Province
    HUANG Yongchun, LI Xinghua, LIN Nan, CHEN Qiang, TANG Hainan, BAI Yue, LI Miaomiao, CAI Zhangyin, YU Hanxiu, XU Jinzhen
    Journal of Fisheries Research    2022, 44 (6): 629-637.   DOI: 10.14012/j.cnki.fjsc.2022.06.012
    Abstract292)   HTML0)    PDF (1819KB)(496)       Save

    From April to December in 2021,68 typical farms and 265 culture questionnaires of diamond shrimp(Penaeus monodon)were investigated in Zhangzhou City,Fujian Province.The results showed that there were mild climate,long sunshine time and long coastline in Zhangzhou,which was suitable for the shrimp culture.The mainly diamond shrimp culture mode in Zhangzhou were using soil pond and higher place pond,where were mainly distributed in Longhai District,Zhangpu County,Dongshan County and Zhao’an County,respectively.There were 2-3 times harvests a year which the stocking density,survival rate,feed coefficient,output and profit were(1.50-4.50)×105 ind/hm2,73.90%,1.63,(0.38-0.98)×104 kg/hm2 and RMB(1.20-12.75)×104 yuan/hm2 in 3-10 salinity mixed by groundwater and bittern in soil pond,respectively.There were 2-3 times harvests a year too,which the stocking density,survival rate,feed coefficient,output and profit were(1.50-6.00)×106 ind/hm2,74.58%,1.84,(0.83-10.20)×104 kg/hm2 and RMB(3.53-11.09)×105 yuan/hm2 using 20-30 salinity natural seawater in higher place pond,respectively.The production of diamond shrimp larvae was increased year by year in Zhangzhou which the increase amount were 7.76% to 39.41% from 2017 to 2020.There was a slightly decline by 32.46% in 2021.At present,the basis and age of the employees of diamond shrimp farmers were poor and old in Zhangzhou,respectively.There was a single specie for selection.The stocking density of higher place pond had been blindly increased,and the aquaculture tailwater was difficult to be treated.The fine management was employed to improve the breeding efficiency,and the "white + black(Litopenaeus vannamei + P.monodon)" healthy breeding mode was popularized for promoting the sustainable and healthy development of diamond shrimp culture.

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    Selectivity of different mesh size codends of two-stick swingnet for Trichiurus lepturus in Eastern Fujian sea waters
    ZHUANG Zhidong, LIU Yong, MA Chao, XU Chunyan, CAI Jiandi, SHEN Changchun
    Journal of Fisheries Research    2022, 44 (3): 235-241.   DOI: 10.14012/j.cnki.fjsc.2022.03.005
    Abstract282)   HTML2)    PDF (804KB)(219)       Save

    In order to explore the selectivity of different mesh size codends of two-stick swingnet for Trichiurus lepturus in Eastern Fujian sea waters,4 mesh sizes(25,35,45 and 55 mm) codends were compared using covered net method in the autumn of 2019.The Logistic model was used to fit the selectivity curve of T.lepturus and the model parameters were estimated by maximum likelihood method.The results showed that:The L0.5 of T.lepturus increased with the increase of mesh size,indicating that the increase of mesh size can effectively release T.lepturus.The relationship between the L0.5 of T.lepturus and mesh size(m) was L0.5=1.877m+45.323(R2=0.712).With the increase of mesh size,the weight escape rate and number escape rate of T.lepturus also increased,both of which were above 60%.

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    Change trend of main water quality indices and pollution identification in Minjiang River basin in recent years
    ZHAO Tianyang, SHI Chengchun, XIE Rongrong, LI Jiabing, JIANG Hua, CHEN Jin, LIU Jihui
    Journal of Fisheries Research    2022, 44 (4): 324-335.   DOI: 10.14012/i.cnki.fjsc.2022.04.003
    Abstract277)   HTML0)    PDF (3847KB)(238)       Save

    The study of water environment pollution is an important means to control watershed pollution.Taking the Minjiang River basin as an example,the trend of water quality change at 16 monitoring stations in the study area from 2004 to 2020 was analyzed by using the Seasonal Kendall Trend Analysis method,and the main pollution identification and analysis of 13 monitoring points from 2017 to 2020 were conducted by using the Time Series Global Principal Component Analysis method.The results showed that the pH and DO of tributaries were higher than those of the main stream,the main stream was lower in the upper and middle reaches and higher in the lower reaches,COD and NH3-N decreased first and then increased in the main stream from the upper reaches to the lower reaches,TP increased first and then decreased.In addition,Kendall’s test showed an improving trend in pH and DO in the study area,while TP and NH3-N were at higher risk of contamination and some sites needed more attention to COD.Finally,Time Series Global Principal Component Analysis was used to identify that the current water pollution pressure in the study area mainly comes from NH3-N and TP,especially the pollution control of tributaries in the basin needed to be strengthened.

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    Reflections on several issues in the construction and management of marine ranching in Zhejiang Province
    MAO Mengpan, CAI Houcai, YE Yongfeng
    Journal of Fisheries Research    2023, 45 (1): 88-95.   DOI: 10.14012/j.cnki.fjsc.2023.01.012
    Abstract260)   HTML1)    PDF (1003KB)(409)       Save

    Marine ranching, as a new form of marine economy, with large investment, high technological content and great development potential, has become an effective means to restore the deterioration of coastal ecological environment and the decline of fishery resources in China, and also an important way to realize the strategy of marine ecological civilization and maritime power. This paper analyzed the development status of marine ranching in Zhejiang Province, pointed out the main problems and their causes in the current construction and management of marine ranching in the province, and put forward some countermeasures and suggestions from the perspectives of updating concepts, industrial integration as well as scientific and technological innovation, which could provide reference for relevant departments.

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    Evaluation of genomic selection against neuronecrosis in the red-spotted grouper
    WANG Zhuobiao, FANG Ming, ZHENG Leyun, GE Hui, CHEN Xinxin, LUO Huiyu, WANG Zhiyong
    Journal of Fisheries Research    2022, 44 (3): 205-212.   DOI: 10.14012/j.cnki.fjsc.2022.03.001
    Abstract240)   HTML4)    PDF (940KB)(369)       Save

    Red-spotted grouper(Epinephelus akaara) is an important maricultured fish in the southeast coastal area of China.It is highly infected by red-spotted grouper nerve necrosis virus(RGNNV) during the artificial cultivation,which seriously restricted the development of the artificial breeding industry.In this study,230 ind RGNNV susceptible(disease-infected death) and 230 ind resistant(eventually survived fish) red-spotted grouper fry were collected for genome resequencing,and 5 412 683 single nucleotide polymorphisms(SNPs) were discovered,which were used for genetic evaluation of the RGNNV resistance of the red-spotted grouper.The estimated heritability of RGNNV was 0.566 2,and the accuracy of the genomic estimated breeding value(GEBV) with whole genome SNPs markers was 0.154 3.The evaluation of the effect of random selection of different numbers of markers on the genomic prediction accuracy showed that using≥5 000(5 k) SNPs markers could obtain more desirable results.This study provided a useful theoretical reference for application of genomic selection for breeding of red-spotted grouper against RGNNV.

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    Preliminary analysis of tidal current dynamic characteristics in Baima Gang sea area of Sansha Bay in Fujian Province
    YOU Yuanxin
    Journal of Fisheries Research    2022, 44 (3): 249-259.   DOI: 10.14012/j.cnki.fjsc.2022.03.007
    Abstract236)   HTML3)    PDF (6658KB)(263)       Save

    Using the quasi-harmonic method,this paper anchored at the six stations in Baima Gang of Sansha Bay in Fujian Province for 27 hours in April 2015 for study of the tidal current dynamic characteristics.The tidal current elliptic factors were computed,while the current type,tidal motion form and the maximum velocity and direction of fractional tide were also analyzed.It demonstrated that the tidal and current type in Sansha Bay was regular semidiurnal tide;the tidal current mainly presented in N-S reciprocating movement;the magnitude of ebb was larger than flood on the main channel,the ebb time was longer than flood;greater at the mouth and smaller at the upper station,the velocity decreased gradually from surface to bottom generally,but performed fuzzy lay phenomenon and circulation structure;the long axis meant value of M2 component tide was between 47~64 cm/s,greater at the mouth and the upper of Baima Gang.

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    An interpretation of the 14th Five Year Plan for fishery development in Fujian Province
    ZHANG Huiping
    Journal of Fisheries Research    2022, 44 (5): 522-528.   DOI: 10.14012/j.cnki.fjsc.2022.05.012
    Abstract235)   HTML0)    PDF (872KB)(274)       Save

    The 14th Five Year Plan for fishery development in Fujian Province is a guiding document for the development of fishery from 2021 to 2025 in Fujian Province,China.During the 13th Five Year Plan period,Fujian’s fishery development achieved good results.Based on the current situation of Fujian’s fishery development,this paper analyzed the development orientation of Fujian’s fishery during the 14th Five Year Plan period,which were focusing on the “big food concept” and ensuring the safe and effective supply of aquatic products,meeting the strategic needs of rural revitalization,serving the construction of a strong marine province,integrating into the construction of an ecological province and serving the overall situation of the reunification of the motherland.The key tasks were to build a “marine granary of Fujian”,promote innovative development of fishery,promote green development of fishery,coordinate the development and security,and expand the cooperation of fishery opening up.This paper also put forward suggestions on strengthening policy support,strengthening project driving,strengthening the construction of the park carrier,and innovating financial support,which would provide a reference for the development of fishery in Fujian during the 14th Five Year Plan.

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    Characteristics of phytoplankton and its Pearson correlation with environmental factors in Pingtan inshore sea area,Fujian Province from spring to summer
    ZOU Shuangyan
    Journal of Fisheries Research    2022, 44 (3): 266-274.   DOI: 10.14012/j.cnki.fjsc.2022.03.009
    Abstract230)   HTML1)    PDF (1237KB)(265)       Save

    According to the monitoring of phytoplankton samples including red tide organisms in Pingtan inshore sea area from April(spring) to June(summer) in 2021,the ecological characteristics of phytoplankton and the Pearson correlation with environmental factors were analysed,in order to explore the changes of red tide organisms in Pingtan inshore sea area. The results showed that a total of 130 phytoplankton species in 4 phyla were identified,which was dominated by diatoms. Species quantity of phytoplankton was in the order of June>May>April. The 13 main dominant species were Skeletonema costatum,Pseudo-nitzschia delicatissima,Chaetoceros curvisetus,Leptocylindrus danicus,Melosira sulcata,Prorocentrum donghaiense,etc. The peak value of phytoplankton cell density was in June. The density ranges from 2.17×104 to 2.11×105 cells/L at different stations,with an average of(6.04~9.85)×104 cells/L. The overall evaluation was in the middle level with the highest level at CCPT04 station in Liushui sea area and the lowest at CCPT02 in Su’ao sea area. According to the Pearson correlation analysis,water temperature,pH and salinity were main factors affecting the phytoplankton community. Red tide disaster prevention should be strengthened in the period of high occurrence of red tide.

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    Estimated breeding value and heritability of 18 pfm body weight for full-sib families of Mastacembelus armatus
    LIU Yanyan, XUE Lingzhan, FU Jianjun, WU Meiying, CHEN Yushu, LIAO Mengxiang, CHEN Duhuang, HU Zhenxi, LIN Xuewen, DONG Zaijie, FAN Haiping, WU Bin
    Journal of Fisheries Research    2022, 44 (3): 213-222.   DOI: 10.14012/j.cnki.fjsc.2022.03.002
    Abstract225)   HTML1)    PDF (1516KB)(235)       Save

    With the gradual rise of aquaculture of Mastacemblus armatus,the problems such as germ plasm confusion and unclear genetic background have become increasingly prominent,which are not conducive to the commercial development of variety.In order to explore the breeding potential of M.armatus,this study used wild M.armatus as parents to construct 17 full-sib families through full-sib breeding model.After 18 months of cultivation,seven effective families were finally obtained.The single trait animal model was used to estimate the breeding value and heritability of each individual by BLUP,with the average body weight of each family as a covariate.The variance component evaluation results of the full-sib family weight of 18 pfm M.armatus showed that the heritability was(0.468 1±0.173 7),the t test reached P<0.01,and the body weight had a positive correlation with breeding value.The regression equation of male was y=0.393 6x-84.193 0(R2=0.755 4,P<0.01);the female was y=0.517x-64.126(R2=0.817 8,P<0.01).The females with the top 50 breeding values were mainly concentrated in family B,E and D,especially family B,the top 24 females and top 19 males were from this family.The maximum breeding value of male fish was 75.09,and that of female fish was 67.49,both of them were from the same family B.Through this study,the M.armatus had high heritability of body weight,which could be selected by phenotype or BLUP breeding value.

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    Research progress of high-value comprehensive utilization of Laminaria japonica
    LI Ying, WANG Yin, CHEN Tingru, LIN Xiaojuan, CHEN Jicheng, LIU Zhiyu
    Journal of Fisheries Research    2023, 45 (2): 192-201.   DOI: 10.14012/i.cnki.fjsc.2023.02.012
    Abstract219)   HTML0)    PDF (1120KB)(381)       Save

    Kelp is one of the major economic algae in China, with abundant resources and annual production ranking among the top in the world.As a medicinal and edible food, kelp is rich in dietary fiber, protein, vitamins, minerals and other nutrients, which is popular with many consumers.The iodine, polysaccharides, active peptides, mannitol and other functional active substances in kelp have physiological function, such as anti-Alzheimer disease, antithrombotic, anti-cancer, anti-inflammation and blood pressure lowering, which can be developed into biomedical products with significant therapeutic effects.In addition, kelp has applications in beauty products, medical textiles, feed and fertilizer, which has a broad development prospect.In this paper, the comprehensive application of kelp in food, biomedical products, feed, fertilizer and other fields as well as the study of active substances were reviewed, with a view to providing reference ideas for developing new kelp products and promoting the high-quality development of kelp industry.

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    Effects of starvation on initial feeding and growth of early Pseudogobio vaillanti larvae
    ZHONG Quanfu, CHEN Bin, FAN Haiping, LIN Yu, ZHUO Yuchen, ZHANG Jingjing
    Journal of Fisheries Research    2022, 44 (5): 484-491.   DOI: 10.14012/j.cnki.fjsc.2022.05.007
    Abstract215)   HTML1)    PDF (1121KB)(144)       Save

    To provide technical support for artificial breeding of Pseudogobio vaillanti,the article explored the effect of starvation stress on the initial feeding and growth,and determined the point of no return(PNR)of early larvae.The changes of absorption of yolk sac,morphology,behavior,growth,survival and initial feeding rate of starved larvae were observed by methods of experimental ecology,and the PNR and optimized initial feeding time were determined.The results showed that under the water temperature of(25.5±1.2)℃,the larvae was started feeding at 4-day-old and the yolk sac was completely absorbed at 5-day-old.A period of mixed nutrition was only maintained 1 day.The initial feeding rate reached 100% at 5-6 days of age.The PNR of starved larvae occurred during 8-9 days of age with the interval between the first feeding to PNR was 4-5 days as the same time of 50% cumulative mortality rate for larvae of P.vaillanti.The total length had negative growth at 9 days of age.At 6 days of age,the body weight of starved group had negative growth and relative fatness decreased continuously,and it was significantly lower than that of control group,which could be used as judgment basis for the starved larvae of P.vaillanti.The results showed that the larvae of P.vaillanti had short mixed nutrition period and weak starvation tolerance.At water temperature(25.5±1.2)°C,the artificially bred larvae of P.vaillanti could be fed at 4-day-old.The best feeding time as 5-day-old,but the latest feeding time should not exceed 7-day-old.

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    Thoughts on carbon sequestration fisheries: a case study of Binzhou City, Shandong Province
    ZHANG Yue, WEI Yanliang, WANG Shanwu, WANG Xiao, YIN Linlin, YANG Beibei
    Journal of Fisheries Research    2023, 45 (1): 96-100.   DOI: 10.14012/j.cnki.fjsc.2023.01.013
    Abstract213)   HTML2)    PDF (933KB)(356)       Save

    Global warming threatens human survival and social development. Reducing the content of greenhouse gases such as carbon dioxide in the atmosphere and developing a low-carbon economy have become the consensus of all countries in the world. Carbon sequestration fishery is of great and practical significance to the development of low-carbon economy. Carbon sequestration fishery will become the driving force of a new round of fishery development. This paper first expounded the concept and mechanism of carbon sequestration and carbon sequestration fisheries. Based on the field investigation of various breeding enterprises and marine pastures in Zhanhua District, Wudi County and Beihai Economic Development Zone of Binzhou City,this paper collected the relevant data of shellfish, algae, fish and shrimp fisheries in Binzhou City in recent years, and then analyzed the current situation of the development of carbon sequestration fisheries in Binzhou City from the aspects of resources, breeding mode, marine pastures and resource restoration. This paper expounded the problems existing in the development of carbon sequestration fisheries, and finally put forward feasible suggestions for the development of carbon sequestration fisheries from the perspective of the government.

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    Preliminary study on transplantation of Tubastraea coccinea in Tong’an Bay,Xiamen
    ZHAO Dongbo, YANG Shunliang, JI Houde
    Journal of Fisheries Research    2022, 44 (3): 275-283.   DOI: 10.14012/j.cnki.fjsc.2022.03.010
    Abstract202)   HTML1)    PDF (7462KB)(239)       Save

    From October 2012 to February 2014,we carried out the transplant experiment of Tubastraea coccinea in Tong’an Bay,Xiamen.After collecting 11 stony coral samples from Taishan Islands sea area in Fuding,we had monthly observations of coral survival,morphology,growth and the ecological factors including water temperature,salinity,turbidity etc,and analyzed the relationship between coral growth,survival and ecological factors by temperature,salt depth turbidity profiler and Vernier calipers as well as other tools.The results showed that,during the observation period,the sea water temperature was 15.27~30.11℃,the salinity was 21.98~30.35,and the turbidity was 2.91~50.31 FTU;the changes of water temperature and turbidity did not cause coral bleaching and death;due to the increase of the amount of water in the sea during storm,the salinity was decreased significantly,and when the salinity dropped below 28,the coral began to turn white,and after a period of time,the coral was severely white and then died.During the transplantation experiments,Tubastraea coccinea had an average growth rate of about 8.2 mm/a.In addition,due to the enrichment of attached marine organisms in Tong’an Bay,the transplanted coral samples were attached to a large number of algae,shellfish and other organisms which inhibited coral growth and seriously caused coral death.In summary,the experimental results showed that,mainly due to the salinity and attachment of biological organisms,Tubastraea coccinea couldn’t survive and grow in Tong’an Bay sea area.

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    Differences in growth,biochemical indexes and digestive enzyme activities between freshwater cultured and seawater cultured Takifugu obscurus juveniles
    CHEN Weijia, LI Fuxue, ZHANG Sipeng, CHEN Yanting, DAI Yanbin, HUANG Honglong, YOU Yingzhe, CHEN Wuge
    Journal of Fisheries Research    2023, 45 (1): 30-39.   DOI: 10.14012/j.cnki.fjsc.2023.01.005
    Abstract201)   HTML2)    PDF (1198KB)(112)       Save

    By comparing the individual growth indicators of Takifugu obscurus juveniles during freshwater cultured and seawater cultured, the activities of digestive enzymes and non-specific immune enzymes, and muscle biochemical components were determined to explore the effects of freshwater culture on growth and physiology of T.obscurus juveniles. The results showed that the body length and body mass growth of T.obscurus juveniles in the freshwater groups and seawater groups were significantly affected by the salinity, and the growth indexes of the juveniles in freshwater group were better than those in the seawater group (P<0.01). The activity of Na+/K+-ATPase was significantly lower than that of the seawater group, and the LZM activity was significantly higher than that of the seawater group (P<0.05). There were no significant differences in the activities of antioxidant enzymes, digestive enzymes and muscle biochemical components between the freshwater groups and seawater groups (P>0.05). Compared with seawater culture, freshwater culture was more conducive to the growth of T.obscurus. This study could provide scientific data for T.obscurus large-scale healthy farming.

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    Analysis on the marine monitoring buoy data variation characteristics and cause of Karlodinium digitatum red tide in Putian sea area, Fujian Province
    LI Qingyu
    Journal of Fisheries Research    2022, 44 (5): 444-458.   DOI: 10.14012/j.cnki.fjsc.2022.05.003
    Abstract198)   HTML3)    PDF (4557KB)(221)       Save

    Based on the marine monitoring buoy data of the red tide process of Karlodinium digitatum in Putian sea area in 2022 and the manual monitoring data,potential reasons of outbreak of red tide were discussed.Before the red tide,the water temperature and salinity in Putian sea area were lower than that in the same period of previous years.With the rapid rise of water temperature and salinity,K.digitatum rapidly proliferated and accumulated to form a red tide under the favorable conditions of weak light conditions,relatively stable hydrological conditions,abundant nutrients(especially ammonia nitrogen)brought by precipitation,and aggregation caused by meteorology and tide.It was suggested that this K.digitatum red tide in Putian sea area might have been triggered by a synchronous appearance of optimal climatic,nutritional and hydrographic conditions.The concentration of chlorophyll-a reached 63.90 μg/L,while DO and pH significantly increased during the red tide,with the maximum value reaching 10.83 mg/L and 8.34,respectively.During the extinction period of red tide,with the continuous decrease of water temperature,wide fluctuation of salinity,the breaking of relatively stable hydrological conditions and the low nitrogen phosphorus ratio in the sea area,the red tide of K.digitatum rapidly disappeared.The K.digitatum red tide originated from Gangnan sea area,and spread to other sea areas with tide and current.Huge mortality of fish has been caused by K.digitatum in Putian sea area,when the cell density was higher than 105 cell/L.

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    Effects of different feeding rates on growth and efficiency of land-based circular pond culture Micropterus salmoides
    MA Yanqun, SI Nan, JIE Baifei, CHENG Guangping, JIANG Linyuan, LI Jianming, DONG Shenghui, GU Changhui, CHENG Man
    Journal of Fisheries Research    2022, 44 (3): 223-228.   DOI: 10.14012/j.cnki.fjsc.2022.03.003
    Abstract198)   HTML1)    PDF (607KB)(285)       Save

    In order to investigate the optimal daily feeding rates of Micropterus salmoides in land-based circular pond culture,five feeding rates were set as 1.6%,1.9%,2.2%,2.5% and 2.8%,and the water temperature of the experimental pond was set as 24.8℃ to 26.5℃.The effects of different feeding levels on the growth and main breeding efficiency of M.salmoides with initial body weight of(116.00±10.68) g were studied.The results showed that the daily weight gain rate,specific growth rate and output per square meter were the highest in B5 group(daily feeding rate 2.8%),followed by B3 group(daily feeding rate 2.2%).There were no significant differences in feed conversion ratio and coefficient of variation between groups(P>0.05).Based on the comprehensive evaluation of main growth indexes and breeding efficiency parameters,the optimal daily feeding rate of M.salmoides with an initial body weight of(116.00±10.68) g was 2.2% in land-based circular pond culture.

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    Study on community characteristic of macrobenthos in coastal area of southern Fujian Province in summer
    HUANG Kun, FU Tingting, HUANG Zhiwei, CHEN Kai
    Journal of Fisheries Research    2022, 44 (4): 312-323.   DOI: 10.14012/i.cnki.fjsc.2022.04.002
    Abstract197)   HTML1)    PDF (3535KB)(132)       Save

    Macrobenthos are benthic ecological groups that live on the bottom or surface of Marine sediments.As an important part of marine ecosystem,macrobenthos usually have weak locomotion ability and are widely used to indicate the quality of their dwelling environment.It is of great value to study the community structure characteristics and diversity index of macrobenthos for the protection of marine environment and biodiversity resources.In order to study the community characteristic of macrobenthos in coastal area of southern Fujian Province,the investigation and sampling of macrobenthos were conducted in summer 2018.The species composition,density,biomass,biodiversity,community structure and Abundance/Biomass Curves of the macrobenthos were calculated and analyzed.The results showed that 126 species in 11 phyla were collected in the survey,most of them were annelids,followed by arachnids and mollusks.The dominant species were Prionospio malmgreni, Scalibregma inflatum and Aglaophamus dibranchis.The density and biomass distribution were very uneven,the mean values were(374±322) ind/m2 and(12.97±23.16) g/m2,respectively.Evenness index,richness and diversity index were at a high level.There was a significant negative correlation between the number of species at each station and pH,salinity,and a significant positive correlation between the number of species at each station and total nitrogen.The richness index was negatively correlated with pH and salinity,and positively correlated with total nitrogen.There was a significant negative correlation between density and salinity.There was a significant negative correlation between biomass and pH,salinity,and an extremely significant positive correlation between biomass and chlorophyll-a.Macrobenthic communities could be divided into four groups.In this study,there was a lack of characteristic species with large body size,and polychaetes with small body size were dominant spieces.ABC analysis showed that the community structure of macrobenthos was stable.Compared with historical data,the density,diversity and richness of macrobenthos in the coastal area of southern Fujian were higher than those in the northern Fujian,and the biomass and evenness were in the same level.The community structure of macrobenthos in the coastal area of southern Fujian was also more complex and the biodiversity was higher.

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    Genetic diversity and genetic structure of Culter alburnus in Tai Lake and Hongze Lake based on mitochondrial DNA Cyt b gene sequences
    YANG Ziping, LI Daming, LIU Yanshan, YANG Jiaxin
    Journal of Fisheries Research    2023, 45 (1): 1-7.   DOI: 10.14012/j.cnki.fjsc.2023.01.001
    Abstract196)   HTML3)    PDF (1775KB)(163)       Save

    Culter alburnus has high nutritional, economic and ecological values, but its wild resources tend to be exhausted in recent years. So, it is urgent to protect germplasm resources of C.alburnus. In this study, the molecular marker of mtDNA Cyt b gene was used to investigate the genetic diversity and genetic structure of C.alburnus populations in Tai Lake and Hongze Lake, and the Cyt b gene sequences were amplified and sequenced. The research results showed that the full length of Cyt b gene was 1 141 bp, and the base content of A+T was higher than that of G+C.29 variable sites were detected and 29 haplotypes were defined in 98 Cyt b sequences. The average haplotype diversity (Hd) and nucleotide diversity (Pi) of C.alburnus were 0.923 and 0.002 74, respectively, and the average number of nucleotide differences was 3.127. The Hd values of Tai Lake and Hongze Lake populations were 0.927 and 0.816, respectively, and the Pi values of two lake populations were 0.002 85 and 0.002 20, respectively. The genetic diversity of C.alburnus showed high Hd and low Pi. The results of molecular variance analysis showed that the proportion of molecular variation within populations was 15.01%, and the proportion of molecular variation between populations was 84.99%. The coefficient of genetic differentiation (Fst) was 0.150 15 (P<0.01), indicating that there was significant genetic differentiation between the two populations. The values of Tajima's D and Fu's Fs neutral tests were both negative, but significant difference was only found for Fu's Fs neutral test. Furthermore, the mismatch distribution curve was obvious unimodal. Both neutral test and mismatch distribution suggested C.alburnus had experienced population expansion in evolutionary history. In a word, the present results could provide scientific data for the management and protection of C.alburnus germplasm resources in Tai Lake and Hongze Lake.

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    Analysis and evaluation of nutritional components in the muscle of Opsariichthys bidens in Min River,Fujian Province
    QIN Zhiqing, LIANG Ping, LIN Jianbin, CHEN Duhuang, QIU Manli, CHEN Zhi, LI Xuegui, GAO Surui
    Journal of Fisheries Research    2022, 44 (5): 508-515.   DOI: 10.14012/j.cnki.fjsc.2022.05.010
    Abstract195)   HTML0)    PDF (705KB)(148)       Save

    In order to provide scientific basis for the nutritional evaluation of Opsariichthys bidens muscle in Min River,the contents of routine nutrition components,fatty acids and amino acids in muscle were determined and analyzed by biochemical method.The results showed that the contents of water,crude protein,crude fat and crude ash in O.bidens muscle from Min River were(75.20±0.14)%,(22.20±0.85)%,(3.15±0.02)% and(1.32±0.01)%,respectively.17 kinds of amino acids were detected in muscle,7 of which were human essential amino acid,accounting for 35.92% of total amino acids(TAA)and 35.46% of umami amino acids(UAA)in TAA.The amino acid score(AAS)of EAA was more than 1.0 except Met+Cys,the chemical score(CS)was more than 0.8 except Met+Cys,the first limiting amino acid was Met+Cys,and the essential amino acid index(EAAI)was 81.37.The content of trace elements in muscle from high to low was K>P>Ca>Na>Mg>Zn,of which the calcium-phosphorus ratio was 1.00∶4.49.To sum up,the O.bidens from Min River was a kind of high quality economic freshwater fish with good edible value and health care function.

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    Application progress and prospect of machine learning applied to fishery research
    ZHOU Wenying, SHI Wenchong
    Journal of Fisheries Research    2022, 44 (4): 407-414.   DOI: 10.14012/i.cnki.fjsc.2022.04.012
    Abstract192)   HTML2)    PDF (955KB)(191)       Save

    The era of fishery big data has come.Some countries have started to carry out fishery big data research.Machine learning is an indispensable and effective means for big data research.Applying machine learning technology in fishery research is very necessary and important to make up for the deficiency of fishery big data analysis and application,and to develop the digital and intelligent fishery in China.The present situation and developing trend of applying machine learning to fishery research at home and abroad were studied by literature analysis.Based on the current research results of machine learning technology in fishery production,fishery classification,water quality detection,target recognition,fishery services and other fields,it was thought that machine learning had played an important role in fishery research.But machine learning was used for micro research more than macro research.And it was used for static research more than dynamic research.It was used even rarer to study interactively with other industries.At present,intelligent fishing products and some fishery observation cloud platforms had emerged.The technologies of Internet of Things also facilitated the widespread use of machine learning in fishery research.Strengthening the hardware infrastructure construction of Internet of Things,and strengthening the cooperation and integration with upstream data were the only approach for machine learning’s being widely applied in fishery research.Now,machine learning had a promising future in the research fields such as fishing activities’ supervision,fishery ecological simulation and producing a variety of daily necessities with fishery products.

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    Current situation and countermeasures of lobster import in China from 2017 to 2021
    YUE Dongdong, LIU Zifei, GENG Rui, WU Xianfang
    Journal of Fisheries Research    2023, 45 (1): 72-79.   DOI: 10.14012/j.cnki.fjsc.2023.01.010
    Abstract185)   HTML2)    PDF (2272KB)(78)       Save

    The fundamental way to fulfill the consumption of aquatic products by Chinese residents is to make overall use of the two markets and resources at home and abroad. Lobster is one of the representative-high-end aquatic products. In order to grasp the characteristics of lobster import in recent years, the article analyzed the trade scale, variety structure, trade mode, source market and other contents by using the import data of lobster from 2017 to 2021 in China. The results were as follows, the import scale of lobster expanded rapidly, with an average annual growth of 11.78% from 2017 to 2021. Fresh lobster was the main type of imported products, and the cumulative import volume accounts for 86.03% of the total import volume of lobster. Ordinary trade occupied an absolute dominant position in China's lobster import trade. 11 countries, including Canada, the United States of America, Australia and New Zealand, and so on, were the main import source markets, and the cumulative import volume accounts for 95.54% of the total import volume. The relationship between income and lobster consumption demand showed that there was a strong correlation between group income and lobster import volume, and the correlation coefficient between group income and import volume was larger. The demand income elasticity of lobster was greater than 1, which was a luxury with elasticity. In order to adapt to China's consumption upgrading and diversified demand, it was proposed to maintain a more open import strategy, make good use of lobster trading tools, and carry out lobster seed industry innovation.

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    Preparation and fatty acids analysis of lipids from Haliotis discus hannai viscera
    SU Yongchang, PAN Nan, ZHANG Bochao, WU Jingna, LIU Shuji, CHEN Xiaoting, CAI Shuilin, LIU Zhiyu
    Journal of Fisheries Research    2022, 44 (3): 242-248.   DOI: 10.14012/j.cnki.fjsc.2022.03.006
    Abstract184)   HTML1)    PDF (637KB)(228)       Save

    To evaluate the nutritional potential of the fatty acids from Haliotis discus hannai viscera,the chemical components were analyzed.The total lipids were extracted with Soxhlet method using petroleum ether and absolute ethanol,then the fatty acid compositions and nutritional values of the lipids were analyzed.The results showed that the contents of protein and crude fat in the abalone viscera were 13.90% and 5.88%,respectively.The lipid yield of petroleum ether method and absolute ethanol method were 1.18% and 1.89%,respectively.The main constitutive fatty acids were C16∶0,C20∶5n-3(EPA),C18∶1n-9 and C20∶4n-6(ARA),with distinguished more unsaturated fatty acids content over saturated fatty acids.The n-3 polyunsaturated fatty acids extracted by petroleum ether took up 18.45% of total fatty acids,which was more abundant than absolute ethanol(15.17%).The fatty acids nutritional evaluation revealed that lipids extracted by petroleum ether possessed better potential bioactivities in cardiovascular disease prevention,including blood lipid decreasing,angiomalacia,inhibitory effects of coronary heart disease and anti-thrombosis.The investigation provided theoretical basis for nutritional evaluation and high-value utilization of H.discus hannai viscera.

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    Discussion on the test of growth performance of new fish varieties(strains)
    ZHONG Guolong
    Journal of Fisheries Research    2022, 44 (3): 284-293.   DOI: 10.14012/j.cnki.fjsc.2022.03.011
    Abstract184)   HTML1)    PDF (1176KB)(292)       Save

    Growth performance is an important character in the breeding of new varieties(strains) of fish,and the factors that affect the growth performance of cultured fish mainly include gene,environment aquaculture activities and human interference.From 1996 to 2021,120 fish breeding varieties were identified in China.but some of them were not applied to commercial.production due to the lack of a relatively unified evaluation standard or specification for testing technology.In this paper,the identification and testing technology status of new varieties(strains) of cultured fish were systematically introduced;the main influencing factors in growth performance test and evaluation were analyzed,and a set of technical suggestions were put forward for establishing test base,test platform,improving test design,strengthening breeding management,reducing human interference,scientific data collection,result evaluation and judgment.It could provide feasible technical reference for the construction of new fish varieties(strains) testing ground,scientific,standardized and fair testing and evaluation of new fish varieties(strains) in China,and could also provide reference for the construction and testing of other aquaculture varieties(strains) testing ground.

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    Design and implementation of Fujian fishing vessel dynamic monitoring and management system
    HUANG Duanqiong
    Journal of Fisheries Research    2022, 44 (5): 516-521.   DOI: 10.14012/j.cnki.fjsc.2022.05.011
    Abstract180)   HTML3)    PDF (4029KB)(219)       Save

    This paper presents the design idea and implementation process of Fujian fishing vessel dynamic monitoring and management system,which mainly includes information management,duty management,emergency management(such as:sudden emergency and typhoon defense),analysis service,“One Chart” and other functions,and analyzes its application effect.The system can provide digital and intelligent scientific decision-making support for the marine and fishery management departments of Fujian Province and can also provide construction ideas for the promotion and application in china.

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    Transcriptome sequencing-based study of gene expression differences in resistance to Aeromonas hydrophila infection in Megalobrama terminalis and Megalobrama amblycephala
    LIU Kai, XIE Nan, DAI Yulai, RUAN Songlin, FENG Xiaoyu
    Journal of Fisheries Research    2022, 44 (4): 301-311.   DOI: 10.14012/i.cnki.fisc.2022.04.001
    Abstract178)   HTML1)    PDF (2344KB)(131)       Save

    To investigate the differences in resistance to Aeromonas hydrophila infection between Megalobrama terminalis and M.amblycephala,the transcriptome of the infected M.terminalis and M.amblycephala was sequenced by the Illumina HiSeq sequencing platform.The results showed that 62 780 Unigenes were obtained from the samples of M.terminalis and M.amblycephala after sequencing and filtering,with an average length of 531.883 bp,and the N50 of Unigenes was 652 bp.The NR and COG database identified 35 443 and 8 632 Unigenes,respectively,accounting for 56.46 and 13.75 percent of all annotated genes.Among the 62 780 Unigenes,33 693 Unigenes were expressed in M.terminalis and 32 936 Unigenes were expressed in M.amblycephala,of which 24 834 Unigenes were co-expressed in M.terminalis and M.amblycephala.Cluster analysis of differential gene expression trends revealed that the gene expression trends of M.terminalis and M.amblycephala at 3 h and 24 h could be divided into 6 categories,with cluster mode 4 containing the most differentially expressed genes(16),cluster mode 1 containing the second-most(15),and cluster mode 5 containing the fewest(4).GO enrichment analysis showed that the differentially expressed genes of M.terminalis and M.amblycephala after infection mainly were concentrated on GO items such as immune response(GO:0006955),antigen processing and presentation(GO:0019882),and membrane(GO:0016020).Enrichment analysis of the KEGG pathway showed that Autoimmune thyroid disease(ko05320),Type I diabetes mellitus(ko04940),Viral myocarditis(ko05416),Cell adhesion molecules(ko04514),and Antigen processing and presentation(ko04612) pathways were highly enriched.The GO and KEGG enrichment analysis showed that the gene expression difference between M.terminalis and M.amblycephala after infection was closely related to antigen presentation genes.This study provided a basis for analyzing the differences between M.terminalis and M.amblycephala immune response during the A.hydrophila infection.

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    Development potential analysis of fishery carbon sink in Fuzhou, Fujian Province
    YU Qinming
    Journal of Fisheries Research    2022, 44 (6): 620-628.   DOI: 10.14012/j.cnki.fjsc.2022.06.011
    Abstract178)   HTML1)    PDF (1329KB)(302)       Save

    At the 75th UN General Assembly in 2020,China solemnly promised the world to strive to achieve carbon peak by 2030 and carbon neutrality by 2060,as a responsible great power.According to Accounting Method for Economic Value of Marine Carbon Sink,marine carbon sink refers to the process,activity and mechanism of absorbing and storing carbon dioxide from air or ocean,such as mangroves,salt marshes,seaweed beds,phytoplankton,shellfish,and macroalgae.Among them,shellfish and algae carbon sink are the main component of fishery carbon sink,and shellfish and algae mariculture are important manifestation of carbon sink function.At present,the production of mariculture shellfish and algae in Fuzhou has always been in the forefront of China,with congenital advantages in developing fishery carbon sink.In addition,since 2022,the development of fishery carbon sink in Fuzhou has been a pilot project,which has achieved many breakthroughs,with huge potential.Based on the current status of shellfish and algae mariculture in Fuzhou,the total amount of fishery carbon sink in recent 10 years was calculated,and the amount of fishery carbon sink in the next 5 years according to the growth trend was predicted in this paper.Compared with other successful cases of marine carbon sink development,the potential of fishery carbon sink in Fuzhou was analyzed,while the existing problems,countermeasures and suggestions were put forward according to local conditions,aiming to provide references for government policy formulation,in order to seek a practical development path for fishery carbon sink.

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    Acute toxicity test of ammonia nitrogen to Litopenaeus annamei juveniles under low salinity and high alkalinity
    SONG Guolin, ZHAO Yingcan, LIU Zhe
    Journal of Fisheries Research    2022, 44 (4): 374-379.   DOI: 10.14012/i.cnki.fjsc.2022.04.008
    Abstract177)   HTML1)    PDF (641KB)(137)       Save

    Under static water conditions with low salt and high alkalinity(salinity 5,total alkalinity 250 mg/L),the acute toxicity test was conducted to examine the effect of ammonia nitrogen on Litopenaeus annamei juveniles with body length of(1.12±0.45) cm.Results showed that median lethal concentrations(LC50) of ammonia nitrogen were 1 001.17,63.41,47.98,28.30 mg/L at the time point of 24,48,72,96 h respectively under low salt and high alkalinity conditions,so that its safe concentrations of total ammonia nitrogen and ionic ammonia nitrogen for Litopenaeus annamei juveniles were 2.83 mg/L and 0.22 mg/L,respectively.Ammonia nitrogen is highly toxic to Litopenaeus annamei,and measures to reduce the content of ammonia nitrogen have been proposed.

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    The effects of different nitrogen and phosphorus nutrients on the growth and fucoxanthin accumulation in Thalassiosira weissflogii
    ZHONG Yue, SU Yongchang, CHEN Youqiang, CHEN Jiannan
    Journal of Fisheries Research    2022, 44 (6): 541-551.   DOI: 10.14012/j.cnki.fjsc.2022.06.002
    Abstract175)   HTML1)    PDF (2385KB)(388)       Save

    By taking Thalassiosira weissflogii as the raw material,the effects of different concentrations of nitrogen and phosphorus nutrients on the growth and accumulation of fucoxanthin in algae were studied in this paper.Under different conditions,the cell density curve was drawn by sampling during the growth,the biomass was measured at the later growth stage,and the fucoxanthin was extracted with organic solvent and detected by the HPLC.The results showed that compared with other nitrogen nutrient treatments,urea group with 0.882 mmol·L-1 concentration was the most suitable for the growth and fucoxanin accumulation of T.weissflogii,and there were significant differences(P<0.05).There was no significant difference in biomass accumulation of 0.054 mmol·L-1 KH2PO4 group,but there was a significant difference in fucoxanthin content accumulation compared with other phosphorus nutrients(P<0.05).Under the condition of nitrogen or phosphorus deficiency,the growth and the accumulation of fucoxanthin in T.weissflogii were seriously affected.This study provided a theoretical basis for the optimization of culture medium and the high yield of fucoxanthin in T.weissflogii.

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    Preliminary experiment on land-based suspension propagation aquaculture of Bangia fuscopurpurea
    JIA Xuli, LIU Bin, CHEN Defu, YE Qiwang, WENG Zutong, LIU Tao, WENG Jincheng
    Journal of Fisheries Research    2022, 44 (4): 356-364.   DOI: 10.14012/i.cnki.fjsc.2022.04.006
    Abstract174)   HTML1)    PDF (5057KB)(123)       Save

    Bangia fuscopurpurea is a kind of high-value economic seaweed rich in EPA and other fatty acids and it is a unique cultured algae in Fujian Province.In order to further promote the cultivation of B.fuscopurpurea and solve the industrial problems of high impurities and rotten food existing in traditional raft cultivation,we conducted the cultivation mode of suspension propagation in kelp phytotron glass room with temperature 9.0~22.0℃ during the period from December 9,2021 to February 10,2022.The results showed that the maximum average daily growth rate could reach 18.18%·d-1 and 21.39%·d-1 in the 50 L film bag air-lift photobioreactor and 1 000 L culture tank,respectively,which the yield was much higher than that in indoor cement pond culture and marine raft culture.Ash content and crude fat separately increased by 20.81% and 25.00%,protein content decreased by 18.15% compared with raft net curtain cultivation,at the same time,the quality of unsaturated fatty acids was also better than that the latter.Land-based suspension propagation aquaculture could effectively avoid the problem of impurity pollution in marine aquaculture which was of great value to improve the processing efficiency and quality of B.fuscopurpurea.In this paper,we reported the land-based aquaculture of B.fuscopurpurea for the first time,which will provide new models and technologies for the facility aquaculture of macroalgae.

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    Optimization of protoplast isolation conditions of Gracilaria tenuistipitata var.liui Zhang et Xia
    HE Yibin, HAO Hua
    Journal of Fisheries Research    2022, 44 (3): 229-234.   DOI: 10.14012/j.cnki.fjsc.2022.03.004
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    Gracilaria tenuistipitata var.liui Zhang et Xia,commonly known as Gracilaria crinale, is one of the major marine economic algae cultivated in coastal areas of China.In order to obtain the best enzymolysis conditions for the isolation of protoplasts from Gracilarla tenuistipitata var.liui Zhang et Xia,the isolation conditions of protoplasts were optimized from the factors of enzymolysis solution composition,enzymolysis temperature and enzymolysis time.The optimum enzymatic hydrolysis conditions were as follows:1) Enzymatic hydrolysis solution components:salinity 25 sterilized natural seawater with 10 mmol·L-1 CaCl2,0.8 mol/L D-Mannitol,2% Cellulase R-10,1% Maecrozyme R-10,0.5% pectinase Y-23,10 U/mL agarase,pH 6.3;2) Enzymolysis conditions:28℃,80 r·min-1,dark enzymolysis for 8 h.This study would provide a powerful tool for the genetic improvement of Gracilaria varieties.

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