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JOURNAL OF FUJIAN FISHERIES ›› 2013, Vol. 35 ›› Issue (2): 106-111.

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Isolation and Identification of Edwardsiella tarda from Cultured Red Sea Bream(Pagrosomus major) with Cryptocaryonosis


  • Received:2013-02-04 Revised:2013-03-11 Online:2013-04-25 Published:2013-04-27



  1. 福州市海洋与渔业技术中心
  • 通讯作者: 刘年锋
  • 基金资助:

Abstract: The Cultured Red Sea Bream(Pagrosomus major) break out a large number of deaths on the marine cage culture area of Donghan town in Fuqing City during August in 2012. The fishes were sick and finally died, with the symptoms of spleen and kidney enlargement, liver congestion in addition to Cryptocaryon irritans disease symptom on the body surface.With the help of TEM,biochemical characteristics of bacteria,and the molecular systematic(phylogenetic tree based on 16S rDNA sequences),the bacteria was identified.It was concluded that the bacteria isolated from the red sea bream above,was Edwardsiella tarda, with the homology of 99%,and together with Edwardsiella tarda in phylogenetic tree,what’s more,with the same biochemical characteristics as Edwardsiella tarda. The test result of the sensitivity to antibiotics showed that the strain chloromycetin senstive and other ten antibiotics senstive.

摘要: 2012年8月,福清市东瀚海区网箱养殖真鲷发生大量死亡事件。病鱼除刺激隐核虫病症状外,还出现脾、肾肿大,肝脏充血等。采用生理生化指标鉴定及16S rDNA系列同源性分析和系统发育树构建等方法,对从病鱼肝脏分离获得的优势菌株进行了鉴定。其生理生化指标符合迟钝爱德华氏菌特点,GenBank中同源序列比对结果显示,该菌与迟钝爱德华氏菌的16S rDNA序列同源性高达99%,系统进化树中与迟钝爱德华氏菌自然聚为一支。药敏实验结果表明:该菌对氟苯考尼等10种药物敏感,对链霉素等4种药物中等敏感。

关键词: 真鲷, 刺激隐核虫, 迟钝爱德华氏菌, Red sea bream(Pagrosomus major), Cryptocaryon irritans, Edwardsiella tarda