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JOURNAL OF FUJIAN FISHERIES ›› 2013, Vol. 35 ›› Issue (2): 100-105.

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Morphological characteristics and molecular phylogenetic analysis of the cultured oyster from the Houhai bay in Putian

Peng-yun WANG   

  • Received:2013-02-01 Revised:2013-02-24 Online:2013-04-25 Published:2013-04-27
  • Contact: Peng-yun WANG

莆田后海养殖牡蛎的形态学观察和分子鉴定

王朋云   

  1. 莆田市水产技术推广站
  • 通讯作者: 王朋云

Abstract: We observed the morphological characteristics and measured the key measurable character indicators of the common oyster cultured in the Houhai bay. The 16S rRNA and CO I were sequenced and the phylogenetic trees involved in seven species of oysters were reconstructed based on the sequence similarities and genetic distances of 16S rRNA and CO I gene sequences in order to identify the isolated samples. Also, the electrophoretograms of isozymes (IDH, SOD) from the digestive gland tissue were analyzed. The results showed that the oyster’s shell shape were different from each other because of the living environment. The oyster from the Houhai bay shoud not be classified as Saccostrea cucullata but Crassostrea angulata. IDH and SOD were found expressing in the digestive gland tissue but no polymorphic loci was detected. The combination of morphological characteristics and molecular phylogenetic analysis has provided a good method to identify the oyster.

摘要: 对从莆田后海采集的人工养殖牡蛎群体样本进行数量性状分析,运用16S rRNA和CO I条形码技术进行分子鉴定,并进行IDH和SOD同工酶电泳分析。结果表明,后海的常见养殖牡蛎贝壳形状多变,品种为葡萄牙牡蛎而非僧帽牡蛎,不同个体的同工酶电泳结果显示为单态。

关键词: 后海, 牡蛎, 形态特征, 分子鉴定, 同工酶, Houhai bay, oyster, morphological characteristics, molecular identification, isozymes

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