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    25 August 2023, Volume 45 Issue 4
    Research Paper
    Construction and evaluation of dissolved oxygen prediction model based on MIC-BP neural network
    CHEN Mengyun
    2023, 45(4):  317-330.  DOI: 10.14012/j.cnki.fjsc.2023.04.001
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    Dissolved oxygen is an important environmental factor that affects the growth of aquatic organisms and water environment. Accurate prediction of dissolved oxygen is beneficial to the healthy development of aquaculture. This study was based on the water quality data and meteorological data of online buoys SK11 and SK18 in Shuikou reservoir area of Minjiang River in Fujian from January to June, 2022. Then, back propagation(BP) neural network prediction model and MIC-BP neural network measurement model were used for machine learning, and the prediction results are given. At the same time, the prediction results of the two dissolved oxygen prediction models were compared and verified. The results showed that after the identification and screening of MIC (Maximum information coefficient), among the 13 input factors, the factors that had great correlation with dissolved oxygen include pH, water temperature, chlorophyll, electrical conductivity, turbidity, ammonia nitrogen concentration and nitrite nitrogen concentration. The effect of the mixed MIC-BP neural network model was obviously better than that of the independent BP neural network model. After the candidate factors were identified and screened by MIC, the performance of the model could be obviously improved. Compared with the independent BP neural network model, the results showed that the performance of MIC-BP neural network model at SK11 station decreased by 29.29%, RMSE decreased by 60.09%,and NSE increased by 27.63%,respectively. At station SK18, MAE decreased by 17.16%, RMSE decreased by 16.23%, and NSE increased by 12.77%,respectively.

    Molecular identification of Oncorhynchus mykiss based on DNA barcode technology
    LI Zhaonan, LI Changzhong, BAO Changhong, HE Caixia, JIN Wenjie, CHEN Yanxia
    2023, 45(4):  331-340.  DOI: 10.14012/j.cnki.fjsc.2023.04.002
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    In order to ensure the healthy and sustainable development of cold-water fish culture industry, and to ensure consumers’ clear consumption and strengthen the market supervision, it is necessary to find an effective method for species identification of salmon and trout. In this study, cytochrome oxidase I gene (COⅠ) was used to develop a simple, economical and efficient molecular identification method, aiming at identifying O.mykiss. The results showed that COⅠ gene could distinguish diploid rainbow trout, Salmo salar, Oncorhynchus keta and Coregonus peled, but could not distinguish golden trout and triploid rainbow trout. In the follow-up study, we will try to identify related species according to other methods. In production practice, this method could provide a gene tag to identify O.mykiss, and provide technical support for its species identification and healthy development of industry.

    Effect of ammonia seawater and 5-HT on the maturation of gametes stripped from Ruditapes philippinarum
    WU Qisheng, QI Jianfei, LUO Juan, NING Yue, GUO Xiang, LUO Huiyu, ZENG Zhinan
    2023, 45(4):  341-345.  DOI: 10.14012/j.cnki.fjsc.2023.04.003
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    To construct the artificial breeding method of R.philippinarum, effects of ammonia seawater and 5-hydroxytryptamine (5-HT) on the maturation of gametes were studied by soaking the dissected gametes in vitro. Results showed that immersion with ammonia seawater and 5-HT significantly stimulated the germinal vesicles breakdown in R.philippinarum (P<0.05). However, the fertilization rate of ammonia seawater treatment group was better than that of 5-HT treatment group. The fertilization rate of oocytes treated with 10 μmol/L 5-HT for 60 min was 28.38%. The fertilization rate of oocytes treated with 0.015% ammonia seawater for 60 min was the highest (about 41.28%).

    Analysis on morphological indices and discrimination of male and female Eleotris oxycephala
    CHEN Yushu
    2023, 45(4):  346-355.  DOI: 10.14012/j.cnki.fjsc.2023.04.004
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    To investigate the morphological differences between male and female E.oxycephala, and improve the breeding efficiency, principal component analysis, R-cluster analysis, stepwise discriminant analysis and T-test were used to analyze 17 direct-measured characteristics and 14 standardized characteristics of 60 wild individuals. The T-test showed that there was a significant difference in size between male and female E.oxycephala individuals, however there was no significant difference in plumpness between male and female individuals. The result of principal component analysis showed that the contribution percentage of the three extracted principal components were 66.989%, 7.803% and 4.901%, respectively, and the cumulative contribution rates reached 79.693%. Standardized characteristics with significant differences of E.oxycephala between male and female populations were selected by R-cluster analysis and stepwise discriminant analysis to establish the sex discriminant equation. The equation was as follows,male:F1=-63.908X1+626.425X2+2 298.275X3+173.546X4 -55.640X5-239.618,female:F2=68.365X1+510.204X2+1 936.135X3+150.444X4 +48.164X5-204.308. The comprehensive discrimination accuracy of male and female individuals was 98.3%. To verify the accuracy of sex discriminant equation, the morphological character data of extra 23 wild E.oxycephala were substituted into the sex discriminant equation, and the comprehensive discriminant accuracy was 82.6%.

    Effects of four non-nutritive feed additives on digestive enzyme activity, immune function and in vitro digestibility of Australian freshwater lobster (Cherax quadricarinatus)
    SONG Xuefei, ZHOU Qing, ZHANG Yingying, ZHANG Zhiyong, WANG Yan, YUN Taihong
    2023, 45(4):  356-364.  DOI: 10.14012/j.cnki.fjsc.2023.04.005
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    The aim of the present study was to explore the effects of four kinds of non-nutritive additives on the activity of digestive enzymes, immune function and in vitro digestibility of liver and pancreas of Australian freshwater lobster (Cherax quadricarinatus), and to screen out the appropriate feed additives, so as to provide a theoretical basis for the application and research of non-nutritive additives in feed. Citric acid (S-Ⅰ), complex enzyme preparation (S-Ⅱ), Lactobacillus plantarum plus Bacillus subtilis (S-Ⅲ), and rhizobium plus B.subtilis (S-IV) were used as the non-nutritive additives to feed Australian freshwater lobster for 14 days. Then, activities of enzymes [alkaline phosphatase(AKP), acid phosphatase (ACP), superoxide dismutase (SOD) and total antioxidant capacity (T-AOC)] as well as in vitro digestibility of feed (dry matter digestibility, protein digestibility and amino acid production rate) were detected. S-Ⅰ could significantly increase (P<0.05) the activities of amylase, AKP and SOD in the hepatopancreas of Australian freshwater lobster, and S-Ⅱ could significantly increase (P<0.05) the activities of amylase, trypsin and immune function. The digestive enzyme and immune function of S-Ⅲ and S-Ⅳ were significantly improved (P<0.05). Among the four non-nutritive feed additives, S-IV had the highest dry matter digestibility, protein digestibility and amino acid production rate in vitro. The digestive enzyme activity, immune function and in vitro digestibility of S-Ⅲ and S-Ⅳ were higher than those of S-Ⅰ or S-Ⅱ, and in vitro digestibility of S-Ⅳ was higher than that of S-Ⅲ, so S-Ⅳ was preferred among the four non-nutritive additives.

    Maximum sustainable yield of Psenopsis anomala in the northern South China Sea
    YIN Yuanyi, ZHANG Lijuan, LIAO Dongsheng, LI Lu, XIE Wenfeng, FENG Bo
    2023, 45(4):  365-371.  DOI: 10.14012/j.cnki.fjsc.2023.04.006
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    According to the stratified sampling survey data of fishery production from fishing ports along the northern South China Sea from 2008 to 2020, the biomass of P.anomala was assessed. The production of P.anomala mainly consisted of trawl net and gill net, accounting for 40.52% and 53.14% of the total catch, respectively. Twelve CPUE data were analyzed by surplus production models. The maximum sustainable yield was 47 402.96 tons inferred by equilibrium Schaefer model. The maximum sustainable yield was 36 739.93 tons inferred by equilibrium Fox model. The maximum sustainable yield was 29 198.74 tons inferred by non-equilibrium Schaefer model. Fishing effort exceeded the maximum sustainable level in double trawl with mainpower above 400 kW, single trawl with mainpower of 100~200 kW and gillnet with mainpower below 50 kW. Overfishing did not occur, and the utilization level was not beyond the optimum level in other fishing methods and mainpower segment. The total allowable catch of P.anomala is comprehensively set within 28~32 thousand tons in the northern South China Sea at present.

    Analysis on the differences of four flavor compounds of Cyprinus carpio var. Quanzhounensis under two aquaculture modes
    HUANG Yin, WEN Luting, DU Xuesong, TIAN Zeyu, LI Zhe, HUANG Bo, WU Xia, LI Qing, QIN Junqi, CHEN Zhong
    2023, 45(4):  372-377.  DOI: 10.14012/j.cnki.fjsc.2023.04.007
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    In order to study the effect of pond and paddy field cultivation mode on the flavor substances of C.carpio var. Quanzhouensis, headspace solid phase microextraction method was used to detect the contents of E-2-nonenal, 2-methylisothiol (2-MIB), and geosmin (GSM) in muscles and hepatopancreas. The content of hypoxanthine nucleotides (IMP) was determined by high performance liquid chromatography tandem mass spectrometry. The results showed that in terms of earthy smell substances, the contents of 2-methylisoalloxal and geosmin in the muscle of C.carpio var. Quanzhouensis cultured in paddy fields were extremely significantly higher than those in pond cultivation mode, and the contents of e-2-nonenal and geosmin in the hepatopancreas of C.carpio var. Quanzhouensis cultured in paddy fields were extremely higher than those in pond cultivation mode (P<0.01). In terms of umami substances, IMP contents in the muscles of C.carpio var.Quanzhouensis cultured in paddy fields were extremely higher than those in ponds (P<0.01); no significant differences were found in the IMP content of the hepatopancreas. In conclusion, C.carpio var. Quanzhouensis cultured in paddy fields had a higher umami taste, but also a heavier earth odor; C.carpio var. Quanzhouensis cultured in ponds had a weak soil flavor, but umami taste was not strong.

    Detection and analysis of ostreid herpesvirus 1 (OsHV-1) in Fujian Province
    JIA Yuanyuan
    2023, 45(4):  378-384.  DOI: 10.14012/j.cnki.fjsc.2023.04.008
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    With the continuous expansion of oyster culture industry, the problem of oyster disease is becoming more and more serious. In recent years, ostreid herpesvirus 1 (OsHV-1) caused mass death of shellfish, which brought great economic losses to shellfish aquaculture industry. OsHV-1 infection occurred frequently in oysters, especially in the seedling stage. In this paper, 90 samples of Crassostrea gigas and C.angulata from six seedling growing enterprises and three sea areas in Fujian were tested for OsHV-1 by PCR and quantitative real-time PCR. The results showed that the average detection rate of OsHV-1 was about 11.11%. Among them, there were 30 seedling samples, while the positive detection rate of seedling samples was 23.33%, which was higher than the positive detection rate of 5% in adult shellfish, and the infection amount of positive samples in seedling was significantly higher than that in adult shellfish. The infection amount of OsHV-1 in C. gigas seedling sample was the highest, reached 6.1×107 copies/mg. This study could provide reference for the detection of ostreid herpesvirus as well as the prevention and control of oyster diseases.

    Review
    Implications of fish conservation in Tibet from the perspective of gut microbiota
    PAN Hongbo, LIU Haiping, GUO Mingxiong
    2023, 45(4):  385-398.  DOI: 10.14012/j.cnki.fjsc.2023.04.009
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    The fish gut microbiota is very diverse, but the information about the type of gut microbiota of different fish is different. Fish live in the water environment, and their gut microbiota is easily affected by factors such as environment, food, and body growth and development. This paper introduced the characteristics and resources of indigenous fish in Tibet, analyzed the composition of gut microbiota of fish, and expounded the main research technical means of fish gut microbiota structure. It could play an important role in the conservation of fish in Tibet.

    Application of environmental DNA metabarcoding in studying biodiversity of zooplankton and phytoplankton
    WU Yongjiang, CAI Mingcheng, SUN Hanchang, HU Huidie, DENG Yaxin, LONG Yinggen
    2023, 45(4):  399-407.  DOI: 10.14012/j.cnki.fjsc.2023.04.010
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    Phytoplankton and zooplankton are the primary producers and consumers of aquatic ecosystems, respectively. Their accurate identification is the basis for aquatic ecology research. Traditional identification of phytoplankton and zooplankton based on morphological characteristics of species is time-consuming and labor-consuming, difficulty in identification, and underestimating species diversity. Therefore, a simple, efficient, accurate and standardized solution for studying biodiversity of zooplankton and phytoplankton is very necessary. Environmental DNA (eDNA) metabarcoding technique has become a common method for studying aquatic biodiversity. It is mainly used in the studying of fish species diversity and has also been gradually used in the studying biodiversity of zooplankton and phytoplankton in recent years. This review firstly introduced the eDNA metabarcoding technique and its sampling method, and then focused on the application of this technique in the studying of biodiversity of zooplankton and phytoplankton to promote the development of eDNA metabarcoding technique in plankton diversity research.

    Discussion and suggestion on developing the polyethylene fishing boat
    CHE Xiaojun
    2023, 45(4):  408-414.  DOI: 10.14012/j.cnki.fjsc.2023.04.011
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    Polyethylene ships have been used and developed for many years in foreign countries, which started relatively late in China. In order to promote the development of polyethylene aquaculture fishing boats and help the development of green aquaculture industry, on the basis of summarizing the fishing boat inspection work and widely referring to published data, the author compared the polyethylene aquaculture fishing boats with the traditional wooden and steel aquaculture fishing boats, and summarized the advantages of polyethylene aquaculture fishing boats in the application. In this paper, the advantages of polyethylene fishing boats for green development of Chinese aquaculture industry were discussed, and the main disadvantages and development difficulties of polyethylene fishing boats were pointed out, while the solutions were put forward. Some suggestions was made in this paper for the development of polyethylene fishing boats, hoping to provide reference for the application and development of polyethylene fishing boats in aquaculture.