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Table of Content

    25 April 2018, Volume 40 Issue 2
    Preparation of marine sources metallothionein based on construction of recombinant strain of Bacillus subtilis
    2018, 40(2):  89-97. 
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    In order to facilitate marine sources metallothionein, safe and high-level expression system as an efficient tool in Bacillus subtilis was reconstructed in this study. The metallothionein (MT) gene was obtained by PCR amplification using Ostrea plicatula genome DNA as a template and sub-cloned into the expression plasmid pHT43 via pMD19-Tvector. Then the plasmid was electro-transformed into B. subtilis WB800N. The molecular weight of expressed fusion protein SUMO-Op-MT was about 23 kD, which was confirmed by Western blotting. This showed that the soluble fusion protein SUMO-Op-MT was expressed in the host bacteria. At the same time, the fermentation broth of recombinant Bacillus subtilis was detected by HPLC, and the results also showed that the soluble protein Op-MT was expressed in recombinant Bacillus subtilis successfully. Furthermore, through the detection of MT activity in recombinant bacteria, it was found that recombinant bacteria’s tolerance to heavy metal cadmium was increased, indicating that active MT was successfully expressed in recombinant bacteria. It provides a theoretical basis for the future development of heavy metal antidote of natural and efficient marine sources.
    Purification and characterization of glutamine synthetase from Alexandrium catenella
    ding guangmao
    2018, 40(2):  98-103. 
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    The crude glutamine synthetase (GS) precipitated by streptomycin sulphate from Alexandrium catenella was purified after subsequently passing through ion-exchange chromatography, Gel-filtration Sephacry1 S-300 and Phenyl-sepharose CL-4B. The molecular weight of GS was 430 kD ,which consisted of 8 subunits with molecular weight of 55.6 kD. GS purified from Alexandrium catenella belonged to GSⅠ. The optimal pH and temperature of maximal GS activity were 8.0 and 25℃, respectively. The presence of Mg2+ resulted in the highest GS activity, while GS activity was fully inhibited in the presence of Fe2+ and Fe3+.
    Effect of xanthophylls and astaxanthin on the skin color and antioxidant capacity of large yellow croaker(Larimichthys crocea)
    2018, 40(2):  104-110.  DOI:
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    This study was to investigate the effect of feed pigment on the skin color and antioxidant capacity of Larimichthys crocea. Four isonitrogenous and isoenergetic diets were formulated by adding 0 mg/kg (D1), 100 mg/kg (D2), 200 mg/kg (D3) and 300 mg/kg (D4) xanthophylls and astaxanthin mixture (1:1) into the basal diet. 1 800 healthy L. crocea with an average body weight of (365.54?5.83) g were selected and randomly divided into four groups with three replicates per group and 150 fish per replicate. The experimental fish were respectively fed four diets for 60 days. The results showed that the yellowness value(b*)of the dorsal and ventral skin of the L. crocea was significantly higher than that of the control group (P <0.05) after 30 d feeding. At the end of the experiment, the redness value (a*) of the ventral skin in D2 group was significantly higher than that of other experimental groups (P <0.05), while no significant difference in the brightness value(L*)and redness value(a*)of dorsal and ventral skin were observed among other groups. Also, no significant differences in the yellowness value(b*)of dorsal and ventral skin were observed among experimental groups (P >0.05), but they were significantly higher than that in D1 group (P<0.05). The total antioxidant capacity (T-AOC), catalase (CAT) and superoxide dismutase (SOD) activities in the liver of L. crocea were increased with the increasing level of pigment in the diet. However, an opposite result was found on the liver malondialdehyde (MAD) content. In conclusion, the supplementation level of xanthophylls and astaxanthin mixture (1:1) for improving the skin coloration and antioxidant capacity of large yellow croaker was suggested to be 100~200 mg per kg diet under this experimental condition.

    The effect of photoperiod and red light on ovary development and oxidative stress status in a stream fish (Acrossocheilus fasciatus)
    2018, 40(2):  111-119. 
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    The aim of the study was to explore the effects of photoperiod and red light irradiation on ovarian development of a stream fish, Acrossocheilus fasciatus. The interactive experiment with two factors and two levels was designed basing on the different photoperiods (12L∶12D and 16L∶8D) and light colors (white and red). Gonadosomatic index (GSI), the proportion of developing oocytes in each stage, plasma melatonin (MT) levels, and malondialdehyde (MDA) contents and superoxide dismutase (SOD) activity of muscle and liver were investigated in the pre-breeding females. The results showed that the maximum of GSI and the percentage of developing oocyte at stage Ⅲ occurred in the fishes under long photoperiod (LP) with red light, but the minimum in those under shot photoperiod (SP) with white light (P < 0.05) (Fig.2). The maximum level of night plasma MT was found under LP with red light condition, while the minimum level was under the SP with white light condition (P < 0.05) (Fig.3). Neither photoperiod nor light color had a significant influence on muscular MDA contents and SOD activities of muscle and liver. However, hepatic MDA contents were significant increased by LP and red light (P < 0.05) (Fig.4). The interaction effect between photoperiods and light colors was detected in the most biological indicators. As a conclusion, long photoperiod with red light could significantly improve ovary development accompanied with the increase of plasma MT level, which suggested that MT played a crucial role in regulating reproduction in this species.
    The effects of stocking density and water temperature on survival and growth of juvenile Brocarded carp
    2018, 40(2):  120-124. 
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    A 30 days breeding test was performed to investigate the effects of stocking density and water temperature on survival and growth of juvenile Brocarded carp. Five stocking density treatments (10, 30, 50, 70, 90 fish/m3) and five water temperature treatments (10, 15, 20, 25, 30℃) were setted, respectively. The results showed that there were no significant difference in other indexes (P>0.05) except the average final total weight between 10 fish/m3 and 30 fish/m3. When the stocking density was greater than 30 fish/m3 and with the increase of stocking density, the average survival rate, final weight, daily gain and specific growth rate of juvenile Brocarded carp were significantly decreased (P<0.05), but the average final total weight and feed coefficient were significantly increased (P<0.05). With the increase of water temperature, the average final weight, daily gain and specific growth rate of juvenile Brocarded carp showed a trend of increasing first and then decreasing, with the maximum value at 25℃ group and significantly higher than other water temperature groups (P<0.05). But the feed coefficient first decreased and then increased, with the minimum value at 25℃ group. Considering comprehensively feed coefficient and yield per unit water, we suggested that the suitable water temperature and stocking density for Brocarded carp cultured in still water pond were 25℃ and 30 fish/m3.
    Study on the technology of the Ulva lactuca SDF’s deproteinization by ultrasound-assisted enzymatic method
    2018, 40(2):  125-133. 
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    In the present study, pH,ultrasonic temperature,ultrasonic time and other parameters on protein removal effects of U. lactuca SDF were studied, with the deproteinization rate and the SDF retention rate of U. lactuca SDF were adopted as indexes. L9(34) orthogonal experiment design was adopted to optimize technology parameters of deproteinization of U. lactuca SDF by ultrasound-assisted enzymatic method, to provide the references of studying it’s physiological activities. The results showed:with alkaline proteinase dosage as 1 400 U/g,U. lactuca SDF solution pH as 11.0,ultrasonic temperature as 50℃,ultrasonic time as 1.5 h under the condition of the 0.6% concentration of U. lactuca SDF and ultrasonic power as 200 W, the deproteinization rate of U. lactuca SDF was (83.91 ± 0.18)% and the retention rate of SDF was (86.23 ± 0.25)%. The effect of deproteinization was better than the nine orthogonal experiment groups.
    Chemical composition and nutritional quality of aquaculture sea cucumber Apostichopus japonicus in Fujian
    2018, 40(2):  134-140. 
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    The present study aimed to analyze the chemical composition and nutritional quality of aquaculture sea cucumber Apostichopus japonicus in Fujian. The content of crude protein, fat, carbohydrate and ash were determined as (83.66 ± 0.17) %, (1.36 ± 0.01) %, (1.82 ± 0.01) % and (11.42 ± 0.26) %,respectively. Compared with other global common trade sea cucumbers, A. japonicus was found to possess better quality with higher protein, lower fat and less ash content. The content of total amino acids (TAA) was (72.13 ± 2.54) %; the ratio of essential amino acids and non-essential amino acids (EAA / NEAA) was (59.41 ± 1.05) %; the ratio of essential amino acids and total amino acids (EAA / TAA) was (37.27 ± 0.41) %; the ratio of flavour amino acids and total amino acids (FAA / TAA) was (45.79 ± 0.41) %; the ratio of medical amino acids and total amino acids was (64.43 ± 0.09)%; the ratio of Lys and Arg was (0.53 ± 0.02); the essential amino acids index (EAAI) was 69.93; the amino acid content was well balanced and fit for FAO / WHO ideal model. 5 common elements and 4 trace elements were detected, among which the major element was found to be phosphorus and iron, respectively. Overall, based on the results obtained, aquaculture sea cucumber A. japonicus was found to serve as a better source of food diet and natural antioxidants.
    Preliminary study on catch composition and biological characteristics of main species of light–liftnet in the Northwest Pacific Ocean
    2018, 40(2):  141-147. 
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    Based on the data investigated by light-iftnet in the Northwest Pacific Ocean in the autumn of 2016, the catch composition and biological characteristics were studied. The results showed that the average daily yield was 13.65t, the highest was 19.14 t appeared in October, the lowest was 10.58 t appeared in August. The average CPUE was 1 822.4 kg·h-1. The highest value was 2 492.2 kg·h-1 appeared in October, and the lowest value was 1 412.8 kg·h-1 appeared in September. The catch species was sixteen, which belonged to 8 order, 12 family and 16 genus. The main catch species were Scomber japonicus, Sardinops sagax , Cololabis saira and Ommastrephes bartrami, accounted for 72.00%, 19.41%, 7.94% and 0.58% in the total catch, respectively. Juvenile fish was accounted for a large proportion in population composition, and the rank of gonad maturity was at a lower level, which indicated that juvenile fish suffered severely damage.
    Analyses on the source and pollution condition of main land-based sewage outlet to the sea in Fujian Province during the 12th Five-year
    2018, 40(2):  148-155. 
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    According to the results of land-based sewage outlets to the sea monitored by marine departments in Fujian Province during the 12th Five-Year, the pollution condition and main pollutants types of land-based sewage outlets except Xiamen area were analyzed. In the analysis the author indicated that the amounts of pollutants to the sea should be adjusted ,because the sewage from thermal power plants and reclamation area was discharged with sea water. In the last the author analyzed the discharging tendency of every kind of pollutant. The results indicated that before the adjustment, the amounts of pollutants discharged to the sea were 21.6 million tones, the amount of pollutants from industrial sewage outlet was larger than others, while the maximum pollutant was suspended solids. After the adjustment, the amounts of pollutants were 5.3 million tones, the amount of pollutants from river sewage outlets was larger. The main pollutants were suspended solids and COD. Some sewage outlets such as Jinjiang Shishi 11 hole bridge sewage outlet, Jinjiang Chendai wubiangang sewage outlet, Ningde City Jiaocheng Municipal sewage outlet, Luoyuan Songshan sewage outlet should be in strictly control because of the amounts of pollutants from these sewage outlets were larger than others. There were many excess pollutants in the sea area of above-mentioned sewage outlets. During the 12th Five-year, the annual amount of sewage from 37 land-based sewage outlets to the sea didn't change strongly, but the amount of COD to the sea decreased year by year, the amount of NH3-N, total phosphorus, oil and heavy metals to the sea showed the decrease trends, while the amount of suspended solids to the sea increased year by year.
    Review on the current situation and perspective of Chinese jellyfish disasters’ research
    2018, 40(2):  156-162. 
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    In recent years, large-scale jellyfish outbreaks have emerged successively in the Bohai Sea, northern East China Sea and southern Yellow Sea. The research that is aimed at the characteristics of jellyfish disasters, will be helpful for defensing and reducing jellyfish disasters. At present, the research on jellyfish disasters in China is still in the primary stage. Although the rapid detection technology of jellyfish disasters using molecular biology has been made advances, the monitoring and early warning technology for Jellyfish disasters is not yet mature. Therefore, the formation mechanism and the drift paths of jellyfish disasters are the current problems that need to be solved urgently.
    Species and research progress of seafood condiments
    2018, 40(2):  163-168. 
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    In this paper, based on the different processing methods of seafood condiments, the seafood condiments are divided into extraction condiments, decomposed condiments and reactive condiments. The study also comprehensively discusses the research progress of these three condiments. It showed the research direction and development trend of seafood condiments. This review aims to develop new seafood condiment and the added value of seafood products, and will provide references for the further research.