Table of Content

25 October 2016, Volume 38 Issue 5

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Study of cultivation technology of the sea cucumber Apostichopus japonicus larvae in offshore net cage

2016, 38(5):  357-362. 

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Under different hatchery conditions, effects of diet and environment on growth and survival of the sea cucumber Apostichopus japonicus larvae were investigated for 7 days by feeding oceanic red yeast or shading net covering. The results showed that the survival rates of the cage hatchery group were over 52.4%~57.9%, which is lower than the indoor hatchery group. When feeding with oceanic red yeast, the larvae growth rate was 86.4 μm/d, the survival rate was 55.1%±8.0%. When feeding with no diets, the growth rate was 85.1 μm/d, the survival rate was 53.0%±7.5%. No significant difference was found between fed and unfed group. The larvae survival rate in net covering group was 52.8%±7.4%, which was significant higher than the survival rate 46.9%±7.0% in uncovering group. These results should be valuable to direct the technique optimization of artificial breeding for the hatchery production of A. japonicus larvae.

Uncertainty evaluation for determination of enrofloxacin residue in aquatic products by liquid chromatography

2016, 38(5):  402-408. 

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When enrofloxacin residue is close to the maximum residue limit in aquatic products, it is very important to carry out evaluation of measurement uncertainty to accurately determine whether the products meet the quality standards. So a method of uncertainty evaluation for the determination of enrofloxacin residue in aquatic products by liquid chromatography was established. According to Evaluation and Expression of Uncertainty in Measurement (JJF 1059.1— 2012) and Guidance on Evaluating the Uncertainty in Chemical Analysis (CNAS—GL06: 2006), a mathematical model was constructed and the uncertainty sources were analyzed, and then the main components of uncertainty were quantified and combined. Finally the expanded uncertainty was achieved. When the measured value of enrofloxacin residue in aquatic products was 97.1 μg/kg, its expanded uncertainty was 5.6 μg/kg. The results showed that the major factors of uncertainty were sample pre-treatment and standard solution. The uncertainties of sample pretreatment and standard solution accounted for more than 50% and 22% of the total uncertainties, respectively.

Identification and serotypes analysis of Vibrio vulnificus from Anguilla anguilla

2016, 38(5):  351-356. 

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Seven strains of bacteria were isolated from the diseased eel, and which were identified as Vibrio vulnificus through 21 kinds of biochemical indexes analysis. The specific primers amplifying hemolysin gene (vlly) of Vibrio vulnificus were designed to detect the isolated bacteria genome DNA, the PCR results indicated that the specific fragments of 352 bp DNA could be amplified from the isolated Vibrio vulnificus, but could not amplify any positive band from non-Vibrio vulnificus bacteria. The mouse serum anti the O antigen of Vibrio vulnificus were prepared, and the assay of agglutination reaction showed that the serum anti- O antigen of Vibrio vulnificus from eel could not agglutinate to the strain 1.1758 of Vibrio vulnificus isolated from human. However, the serum anti O antigen of FJ03-X2 could agglutinate to the most strains of Vibrio vulnificus from Anguilla anguilla. These results suggested that the PCR primers based on hemolysin gene could specifically detect the strains of Vibrio vulnificus, the serovar of Vibrio vulnificus from eel was different from other sources, and the strain of FJ03-X2 had good immunogenicity O antigen and could be as candidate strain for vaccine development of Vibrio vulnificus.

Investigation on nekton resources of spring in the west sea areas of Xiamen

2016, 38(5):  386-393. 

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Based on the survey data of nekton resources in the west sea areas of Xiamen in March 2015，a preliminary study was made on the characteristics of nektons in the areas. The results showed that the total kinds of captured nektons was 98，which consisted of 51 kinds of fish，16 kinds of shrimps，23 kinds of crabs，5 kinds of stomatopods and 3 kinds of cephalopods. Charybdis japonica, Exopalaemon carinicauda, Coilia mystus, Johnius belengerii and Parapenaeopsis hardwickii dominated in the areas. The average values of Margalef richness index，Shannon Wiener diversity index and Pielou uniformity index were as follows: 5.358，2.365 and 0.732. The average biomass of captured nektons was 307.068 kg∕km2 .The biomass of fish，shrimps，crabs，stomatopods and cephalopods were 192.749，28.782，72.710，7.869 and 4.958 kg/km2，respectively. Compared with the historical data，the species and the resources of nektons decreased obviously. It is necessary to strengthen the management and protection of fishery resources.

Discussion on the influence of offshore wind farm construction on fishery production and the countermeasures in Fujian Province

2016, 38(5):  415-418. 

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Through summarizing the overview of offshore wind farm development in Fujian Province, we analyze the impact of offshore wind farm construction on mariculture, marine fisheries and fishing operations, furthermore, the ecological compensation and restoration for fishery resources are proposed to mitigate the impact of offshore wind farm on fisheries production activities. Also, it can provides a theoretical basis for the offshore wind farm construction and helps provide reference points in management approval for marine management departments.

A Meta-analysis:the effect of three kinds of Chinese herbal medicines for tilapia Streptococcus agalactiae

2016, 38(5):  409-414. 

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Through searching literatures about the effect of Chinese herbal medicine to tilapia Streptococcus agalactiae from CNKI、VIP、DUXIU、WANFANG databases, the paper used RenMan 5.3 to conduct Meta analysis. The effect of Rheum, Rhus chinensis and Coptis chinensis three kinds of Chinese herbal medicines on tilapia Streptococcus agalactiae were statistical evaluated. The study would provide scientific references for screening Chinese herbal and formulating Chinese medicine compound to prevent and control tilapia Streptococcus agalactiae. Bring into two literatures for Meta analysis, the effect of three kinds of Chinese herbal was: Coptis chinensis> Rheum, Coptis chinensis>Rhus chinensi, Rhus chinensis> Rheum. Both differences were statistically significant，result was solid. The effect of three Chinese herbal for tilapia Streptococcus agalactiae was Coptis chinensis>Rhus chinensi> Rheum. The results need further validation in the process of cultivation.

Yueqing Bay fishery waters and germ plasm resource conservation area monitoring and assessment in 2015

2016, 38(5):  377-385. 

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Using 2015 seasons of the North of Yueqing Bay seawater culture areas and Yueqing Bay germ plasm resources environmental quality monitoring results, we analyzed and evaluated the fishery water quality of two areas and sediment quality condition, and compared with the monitoring results made in 2014. The results showed that the the overall water quality of main fishing areas in the city in 2015 could meet the demand of the fishery water quality function, two overall water quality in monitoring areas were consistent with that of last year. The main over standard indexes of affecting the water quality comprehensive evaluation were inorganic nitrogen, active phosphate, petroleum and volatile phenol. The mainly over standard indexes of sediments were heavy metal copper and fecal coliform. Water quality index(WQI) of the north of Yueqing Bay seawater culture zones is medium throughout the year , WQI of Yueqing Bay germ plasm resources is good throughout the year.

Ultrasonic assisted extraction of loach polysaccharides process optimization

2016, 38(5):  372-376. 

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Using loach as a raw material,adopting ultrasound-assisted extraction technique of polysaccharide loach, and using sulfuric acid - phenol assay of polysaccharide, the orthogonal experimental were designed through single factor to explore the effect of solid-liquid ratio, ultrasonic time and ultrasonic power on loach polysaccharide extraction rate. Through process optimization, the optimal technique by ultrasonic extraction of loach polysaccharide was obtained that material liquid ratio 1∶20, ultrasonic time 20 min, ultrasonic power 100 w. The extraction rate of loach polysaccharide was the highest under the optimum conditions, reaching 2.79%.

Study of processing technology for instant leisure food of read-to-eat Ruditapes philippinarum

2016, 38(5):  363-371. 

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In this paper, the influences of the key processing parameters, including the deodorization, pickling, color-protecting and drying on the quality of the ready-to-eat Ruditapes philippinarum products were evaluated by taste and flavor, moisture content and texture properties. The optimum process conditions of Ruditapes philippinarum leisure food were carried out by single factors and orthogonal combination experiment. It was concluded that the deodorization process parameters were 2.0% jasmine juice adding 2.0% ginger juice, time 80 min; Pickling process parameters were 5% salt, 2% monosodium glutamate, 8% sugar, time 4 h; Color-protecting parameters were 0.10% D - ascorbic acid sodium, time 1 h; Drying process parameters were temperature 75 ℃, time 3 h. In this condition, the instant Ruditapes philippinarum products were special in flavor, and good in taste.

Determination of 15 polycyclic aromatic hydrocarbons (PAHs) residues in aquatic products by high performance liquid chromatography

2016, 38(5):  394-401. 

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The testing conditions were optimizing and improving based on QuEChERS technique combined with high performance liquid chromatography(HPLC), which is frequently used for pesticide detection in aquatic products. In the optimized conditions, 15 polycyclic aromatic hydrocarbons (PAHs) residues in aquatic products were separated and detected by HPLC. The calibration curves were linear in a concentrations range of 1~50 ng/mL for 15 kinds of polycyclic aromatic hydrocarbons with correlation coefficients over 0.995. As (S/N)≥3, the limits of detection was 0.1~2.0 μg/kg. The average recovery ranged from 75.8% to 101% and the relative standard deviation was below 15.0% for spiked shrimp sample with polycyclic aromatic hydrocarbons. Finally, QuEChERS-HPLC was applied for PAHs detecting in practical samples and present that this method was simple and sensitive to be suitable for PAHs determination in aquatic products．

Review on disease control researches of marine fish NNV

2016, 38(5):  419-426. 

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Nervous necrosis virus (NNV) can lead to nervous, encephalopathy or retinopathy necrosis in marine fishes. The virus causing the nervous necrosis disease in marine fishes mostly infects the grouper fries and fingerlings, the strong pathogenicity and high mortality rate is a hammer blow for the marine fish culture, which had become one of the most important restrictions of marine culture industry. The study mainly reviews on the research progresses of basic characteristics, diagnostic techniques, transmission, vaccine immunology and controlling strategies of NNV. This paper will provide the references for preventing and controlling the NNV disease of marine fish culture industry.

Advances in the study of sulfur dioxide removing technics from aquatic products

2016, 38(5):  427-430. 

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The extra additives of sulfur dioxide in aquatic products are very common and serious problems, which have caused Chinese aquatic product export to suffer from heavy loss in the recent years. The paper reviewed the hazards of excessive addition of sulphur dioxide, the origin of sulphur dioxide and the sulphur dioxide removal technics in aquatic products. In accordance with the base of former study and the result of the author's practices, the irradiation treatment to degrade the residue of sulfur dioxide in aquatic products was proved to possess broad application foreground.