Table of Content

25 August 2019, Volume 41 Issue 4

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SSR and SNP analysis based on Trichiurus japanicus transcriptome

2019, 41(4):  269-277. 

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In order to fully explore the genetic resources and develop the microsatellite markers of of Trichiurus haumela, SSR and SNP was screeed. and the analyzed respectively by MISA software，Samtools and GATK which was based on transcriptome sequencing data of Trichiurus haumela. Results showed the total numble SSRS were of 49311, including 44,625 complete microsatellites. Composite SSRS were 4,687. The length of SSR segment ranges from 11 to 374. There were 30,997 sequences with repetition length less than 20bp. Among them, there are 7,523 SSRS with length between 12 and 20bp, accounting for about 29.35% of the total number, showing medium polymorphism. And 13627 long sequences with repetition length greater than 20bp, accounting for about 53.18% of the total number, showing high polymorphism. A total of 876199 SNP bits were obtained, including 335218 transversals, 540981 transversals, and the ratio of transversals to transversals was 1.61:1. The number of SNPS in the non-coding region was 578020. There are a total of 298,179 SNPS in the coding area. Among the base conversion types in the coding area, C-> T accounts for the highest proportion, which is 22.09%. To sum up, this study of characteristic analysis SSR and SNP of Trichiurus haumela could provide important scientific basis for the development of molecular markers, the construction of genetic linkage map and the protection of germplasm resources in the future.

Study on the growth of Prorocentrum donghaiense by using different organic phosphorus

2019, 41(4):  278-283. 

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Prorocentrum donghaiense was cultured with PM4A multi-phosphorus plate (a total of 59 phosphorus sources) to study the effect of different phosphorus forms on its proliferation.Results showed that the 47 kinds of phosphorus source (including 42 organophosphorus and 5 inorganic phosphorus) could be utilized by Prorocentrum donghaiense. Prorocentrum donghaiense had higher uptake efficiency for organic phosphorus than that for inorganic phosphorus, mainly for organic phosphorus containing C-O-P ester bonds, including phospho-glycolic acid, D-mannose-1-phosphate and phosphoenol pyruvate.These indicated that organic phosphorus may be an important phosphorus source affecting the growth of algae, providinga scientific basis for the outbreak and solutions of Prorocentrum donghaiense red tide, which was of practical significance.

Effects of shelter type and benthic microalgae on growth and survival of sea cucumber Apostichopus japonicas Selenka in earthen ponds

2019, 41(4):  284-292. 

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Outdoor experiments were conducted to investigate the sea cucumber (Apostichopus japonicas Selenka) in earthen ponds. Four kinds of shelter (scallops cage, woven bags, sunshade nets and hollow brick) and the changes of species composition, density and biomass of benthic microalgae were analyzed, while the effects of four kinds of shelter on growth and survival of sea cucumber were also studied. The results indicated that the abundance and distribution of diatoms determined the total variation of the benthic microalgae abundance. The average lever of benthic microalgal biomass were 3.46, 2.46, 2.77 and 3.82 μg.cm-2, respectively. There are significant differences of sea cucumber’s growth and survival rate among the four kinds of shelter. The growth of sea cucumber on hollow brick was the slowest, but the survival rate (66.5%) and the yield (192.8 g?m-2) were the highest. The survival rate of sea cucumber on scallop cage was 48.7%, and the yield of it was 169.8 g?m-2, which were only next to hollow brick. The survival rate and yield of sea cucumber on woven bag or sunshade net were low. The results showed that the survival rate and the yield of juvenile sea cucumber in earthen ponds was closely related to the biomass of benthic microalgae on the shelter.

Spatial distribution of macrobenthic diversity factors in Xiamen Bay and its relationship with environmental factors

2019, 41(4):  293-301. 

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In order to study the spatial distribution of biodiversity factors of macrobenthos in Xiamen Bay and the correlation with environmental factors, the investigation and sampling of macrobenthos in Xiamen Bay were conducted in August 2012. The species number, diversity index H ', AZTI Marine biological index AMBI and multi-variable AZTI Marine biological index M-AMBI were calculated and analyzed, and the correlation between each index and environmental factors was calculated using SPSS software. The results showed that there were 103 species in 8 phyla in the survey ares, with the average species number at each station is 11, the average diversity index H ' is 2.328, the average AMBI is 1.739, and the average M-AMBI is 0.538. There was a significant or extremely significant correlation between the number of species at each station and TOC, salinity, pH, DO and COD, and a significant or extremely significant correlation between AMBI and sulfide, salinity and petroleum hydrocarbons. The diversity index H 'and M-AMBI of each station were significantly or extremely significantly correlated with sulfides, petroleum hydrocarbons, organic carbon, salinity, pH, dissolved oxygen and chemical oxygen demand.

Status of fishery resources in Xenocypris davidi National Aquatic Resource Reserve of Luokou River

2019, 41(4):  302-309. 

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In order to scientifically formulate fishery resources protection and management measures, the status of fish resources in Xenocypris davidi National Aquatic Resource Reserve of Luokou River was investigated in March, July, October and December of 2018. The results showed that there were 36 species in the reserve, belonging to 4 orders, 9 families and 31 genera. Among them, Pseudogastromyzon fasciatus was endemic species in Fujian Province, and Anguilla sp., Elopichthys bambusa, Squaliobarbus curriculus and other 16 species were the national key protection of economic fish. Fish faunal characteristics of the reserve are mainly river plain components, omnivorous and drifting eggs. Xenocypris davidi, Culter alburnus, Pseudobagrus fulvidraco, Tilapia sp., Siniperca kneri and Megalobrama terminalis were the dominant species. Plagiognathops microlepis, Cyprinus carpio and Hypophthalmichthys molitrix were the normal species. The genus and family diversity index (G-F) was 0.13 and Shannon-Wiener index was 2.32.

The interannual changes and present situation of marine environment in the estuary of the Mulan River and the Qiulu River

Li ZHANG

2019, 41(4):  310-316. 

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Changes in the environment of the sea entrance areas of Mulan River and Qiulu River in Xinghua Bay were evaluated through the analysis of eleven years of monitoring data, combined with the changes of the pollution sources. The environmental quality in 2018 was also evaluated in order to provide reference for the remediation of Mulan River watershed in 2019. The results of the evaluation were as follows: 1) The environment of the sea entrance areas of Mulan River and Qiulu River in Xinghua Bay had no improvement from 2008 to 2018. DIN and DIP were still the main pollutants. 2) The content of heavy metals in water environment did not exceed the standard. Hg (2009a) and Cu (2018a) in the sediments exceeds standard. Environmental pressure sediments of heavy metals were higher than water. Higher levels of Cu and Hg in water and sediments than other heavy metals was observed. 3) The seawater quality of the sea entrance areas of both rivers exceeded the standard in 100% of the samples in 2018. The main pollutants were active phosphate and inorganic nitrogen. It was more prominent in the sea entrance area of Mulan River. The nitrogen in the water, phosphorus and sediment in that area were all exceeding the standard, while other evaluated indicators could still meet the needs of marine functional zoning.

Research on the processing technology of Mytilus Edulis complex condiment

2019, 41(4):  317-325. 

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The cooking liquor and minced meat of Mytilus edulis were used as raw materials to produce the enzymatic hydrolysate as the base of complex condiment. The optimum off-flavor removing conditions were obtained and the optimal seasoning process was confirmed using sensory evaluation and identifying of different flavors with electronic nose. The results indicated that the complex condiment flowed appropriately and had delicious taste. The optimum complex condiment ingredients were as follows: papain of 0.5%, animal protease of 0.5%, temperature 55℃, pH 6.5, hydrolysis time 5h, deodorization sauce of 4.0%, and spices extrication 6.0%.

Effects of different packing materials on pigment content of Porphyra haitanensis during storage

2019, 41(4):  326-332. 

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Effects of different packaging materials on pigment of laver during storage were discussed to provide references for improving quality and prolonging storage of laver. Using Porphyra haitanensis as experiment material, the contents of chlorophyll, chlorophyll-a and carotenoid and the contents of phycoerythrin, phycocyanin, allophycocyanin and phycobiliprotein from Porphyra haitanensis were determined every seven days during storage at (25±1)℃ constant temperature. Compared with the single material packaging of PET, LDPE, HDPE and PA, the composite packaging material of PA/PE delayed the decrease of chlorophyll, chlorophyll-a and carotenoid contents, PET/PE inhibited the decline of phycoerythrin, phycocyanin, allophycocyanin and phycobiliprotein contents. The effects of composite packaging material of PA/PE and PET/PE on the color retention of Porphyra haitanensis were better than the single material packaging of PET, LDPE, HDPE and PA.

Summary of critical control points in processing of ready-to-eat jellyfish

Shui-Fen TANG

2019, 41(4):  333-338. 

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According to the principle of hazard analysis and critical control points combined with the characteristics analysis of ready-to-eat jellyfish processing, the critical control points and limits of ready-to-eat jellyfish were identified by analyzing the potential physical, chemical and biological hazards in the processing of ready-to-eat jellyfish. At last, the preventive measures were proposed to minimize or effectively eliminate the hazards in the processing of ready-to-eat jellyfish, and provide a theoretical basis for improving the quality and safety management of ready-to-eat jellyfish.

Design and preliminary experiment of long-line abalone culture facility in the bottom waters outside the bay

2019, 41(4):  339-345. 

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Abalone culture in Fujian Province is concentrated in the coastal bay. But the survival rate of abalone culture has dropped sharply from over 90% to around 50%, because of the serious ageing of water and sediment as well as the destruction of water environment by decades of large-scale high-density disordered farming. The economic benefits of abalone farming and industrial development has been seriously affected. The areas outside the bay are very suitable for abalone culture due to their advantages of broad space, high water exchange rate, strong self-purification ability and dark excellent water quality. However, the biggest challenge of safe aquaculture is the poor performance of existing abalone farming facilities and the harsh marine environment outside the bay. A facility named Long-line Abalone Culture Facility System in the bottom waters of the bay has been developed by the author which has the ability to avoid typhoon damage. The equipment consists of two parts: long-lined Abalone culture facility and mechanized production equipment. This paper reports the design and preliminary experiment of the long-lined Abalone culture facility. The preliminary experiment showed that the design proposal is reasonable owing to the ability to avoid typhoon damage and operate mechanically.

Analysis on aquacultural modes of abalone in Fujian Province

Jian pingKANG

2019, 41(4):  346-352. 

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Fujian is a major province for abalone culture in China. In recent years, with the development of abalone aquaculture, the problems of abalone aquaculture have gradually become prominent in Fujian. In order to promote the development of abalone aquaculture in Fujian, this paper summarized the abalone farming modes, including industrial aquaculture, shallow sea farming, North-South relay and offshore facility farming.

Application of shells and preparation of shell materials

2019, 41(4):  353-358. 

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Based on shells’ components, structures and characters, this paper made a review of a wide variety of shell materials’ manufactures and applications, and divided their functions into several parts. In order to make full use of shell materials, this paper emphatically introduced shells’ applications in aspects of absorbing CO2, harnessing pollution, and sterilizing. Meanwhile, it demonstrated preparation and practice of shells’ composites and biomimetic materials. Contrasted with limestone, which is a traditional material, shells are far easier to obtain. Due to shells’ various kinds of advantages, utmost significance should be attached to developing shell materials.