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Table of Content

    25 December 2017, Volume 39 Issue 6
    Prediction and bioinformatic analysis of miR-34 target genes in Scylla paramamosain
    2017, 39(6):  419-428. 
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    Mir-34 is a family of microRNAs that is highly conserved in evolution and is involved in many important life activities. In this study, the sequences characteristics between spa-miR-34 of Scylla paramamosain from the database constructed by our laboratory and miR-34 of other animals from the miRBase were compared, and then the spa-miR-34 target genes were predicted by two online tools: RNAhybrid and Segal Lab. The functional annotation and signal pathway enrichment of these target genes were analyzed using blast2go software. The results showed that the number of the predicted pa-miR-34 target gene was 66, and the functions of these genes are mainly distributed in the biological processes of oxidation-reduction, phosphorylation, calcium ion transmembrane transport, transcription, etc. Signal pathways related to these genes are mainly concentrated in the metabolism of arginine and proline metabolism, antibiotic biosynthesis, phenylpropion biosynthesis, glycolysis and glycogenation, and other synthetic metabolic pathways. The predicted gene family and pathways of the spa-miR-34 target genes also include CHH family, ERK pathway, cell cycle and UPP pathway, and SUMO pathway. In addition, ecdysteroid receptor gene was also one of the predicted target genes. It is speculated that spa-miR-34 plays an important role in the reproduction, molt and the metabolism of amino acids and other substances. This study provides an important reference for further understand the role of spa-miR-34 in S. paramamosain.
    The genotyping and molecular evolution analysis of nervous neurosis virus (NNV) in infected Epinephelus coioides in southern Fujian
    2017, 39(6):  429-436. 
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    In this study,we used the nervous neurosis virus ( NNV ) test standard of Office International Des Epizooties (OIE) to detect the viral nervous necrosis of Epinephelus coioides collected from southern Fujian. All the six samples were detected to be infected with NNV. The positive clones were separately sequenced from both forward and reverse directions with vector primers,and the sequences were used to construct the phylogenetic trees. The virus particles were isolated and purified by PEG method. All the sequence information of positive clones was utilized to analyze the selection pressure of viral coat protein gene. The results showed that the collected six samples were infected with RGNNV,indicating that the infectious NNV of grouper was mainly RGNNV in southern Fujian. The vims was isolated and purified to obtain a kind oi icosahedral vims paricles with a diameter of 25~28 nm. Molecular evolution analysis showed that the NNV coat protein gene experienced the purification selection,indicating that the virus retained very low genetic variation.
    mpacts of natural seawater and artificial seawater on the growth performance and expression of metabolic genes of Pacific white shrimp
    2017, 39(6):  437-443. 
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    The quality of seawater is one of the most important environmental factors affecting the reproduction,development,metabolism and growth of Pacific white shrimp (Litopenaeus vannamei) . Therefore,knowing the impacts of different types of seawater( natural seawater and artificial seawater) on white shrimp aquaculture will provide important information for the aquaculture industry. In the present study,rearing experiment and qPCR were conducted to investigate the impacts of two types of seawater on the growth perfoniance and expression of metabolic genes of Pacific white shrimp. The results obtained showed that P. aibuhitensis reared in natural seawater had significantly greater body length,body weight,head length,head width,muscle weight,and body circumference ( P < 0.05 ) .In addition,except the expression of IDH gene of female shrimps,the gene expression of PK,HK,IDH and CPT1 were generally depressed in the artificial seawater groups ( P<0.05 ),which may partially explain the hampered growth performance detected.
    Screening of marine enzyme producing yeast and its application in aquaculture
    2017, 39(6):  444-454. 
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    With the continuous development of aquaculture in our country,the traditional feed have been unable to meet the development needs of aquaculture due to its high cost,unstable quality and other factors. It is urgent to develop new type feed for aquaculture microorganism preparation. Screening yeast strains which are capable of secreting extracellular digestive enzymes are of great significance for the development and application of aquaculture. In this study, 44 strains of yeast strain which could secrete extracellular digestive enzymes were isolated and screened from marine sediments. Among them,there were 15 strains which could produce amylase,12 strains could secrete protease,21 strains could secrete extracellular lipase, and 14 strains could secrete cellulase. It was worth mentioning that there were two yeast of HY-27 and HY-37 could secrete all four digestive enzymes,then the author conducted a preliminary identification of the two strains of yeast. the identification results were Sporidiobolus pararoseus (HY-27) and Sporobolomyces patagonicus (HY - 37 ).
    Acute toxicity of six drugs to Fugu bimaculatus
    2017, 39(6):  455-462. 
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    The acute toxicity of six common drugs, including potassium permanganate, povidone-iodine, formaldehyde, glutaraldehyde, trichlorfon and cupric acetate, to Fugu bimaculatus were studied at water temperature of (27±1)℃, salinity of 25±1, pH of 7.90~8.10 and dissolved oxygen level of above 6.0 mg/L using static bioassay test. The results showed that the lethal concentration 50 (LC50) of potassium permanganate to F. bimaculatus for 24, 48, 72 and 96 h were 1.88, 1.84, 1.76 and 1.75 mg/L, respectively; the 24, 48, 72 and 96 h LC50 of povidone-iodine were 23.30, 22.55, 20.34 and 19.50 mg/L; the 24, 48, 72 and 96 h LC50 of formaldehyde were 11.27, 7.06, 6.38 and 5.57 mg/L; the 24, 48, 72 and 96 h LC50 of glutaraldehyde were 9.25, 9.03, 7.03 and 5.48 mg/L; the 24, 48, 72 and 96 h LC50 of trichlorfon were 16.85, 14.19, 10.23 and 7.92 mg/L; the 24, 48, 72 and 96 h LC50 of cupric acetate were 10.54, 8.78, 6.13 and 5.46 mg/L. The safe concentration was found to be 0.53 mg/L in potassium permanganate, 6.34 mg/L in povidone-iodine, 0.83 mg/L in formaldehyde, 2.58 mg/L in glutaraldehyde, 3.02 mg/L in trichlorfon and 1.83 mg/L in cupric acetate. Order of sensitivities of six drugs to F. bimaculatus was: povidone-iodine > trichlorfon > glutaraldehyde > cupric acetate > formaldehyde > potassium permanganate. The using of six drugs should be controlled strictly in production according to safe concentration.
    Compensatory growth and biochemical composition after experiencing cycles of feed deprivation and re-feeding in juvenile brocarded carp
    2017, 39(6):  463-468. 
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    A 40-days feeding trial was conducted to determine compensatory growth and biochemical parameters after experiencing cycles of starvation and re - feeding in juvenile brocarded carp [body length (10.63±0.20 ) cm, body weight (17.63±0.72 ) g ] reared at (27.0 ±1.0)℃. Four feeding regime groups were designed as follows : starved 1 d ,then fed 3 d; starved 1 d,then fed 5 d ; starved 2 d,then fed 3 d and starved 2 d,then fed 5 d,while one control group fed daily. The results showed that the specific growth rate and feeding rate of 1 day of starvation followed by feeding groups were significantly higher than control group(P<0.05),while the difference of final average length,final average weight,total food ration,weight gain rate and feed efficiency were not significant (P>0.05),showing a completely compensatory growth,and it is presumed that compensatory growth should be achieved by increasing the feeding rate. The final average length,final average weight,total food ration,specific growth rate and weight gain rate of 2 days of starvation followed by feeding groups were significantly lower than control group (P<0.05),while the difference of feeding rate and feed efficiency were not significant ( P>0.05),showing a non - compensatory growth,and whether the damage to its physiological function needed to be further studied and determined. Compared with control group,the body protein and lipid of four feeding regime groups were metabolized and replaced by moisture and ash. Based upon the effect of compensation and actual days of feed intake,the cyclic feeding regime of starved 1 d,then fed 3 d could be recommended for the brocarded carp rearing under the present experimental conditions.
    Optimization of spray-dried clam (Ruditapes philippinarum) cooking liquor for powder
    2017, 39(6):  469-475. 
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    A large amount of byproducts of liquor were produced in the process of cooking clam. In order to develop them into the seasoning powders,the clam cooking liquor was spray - dried after enzymatic hydrolysis and concentration. On the basis of single factor study,the following response surface method was used to optimize the parameters of inlet temperature,atomization pressure and total solid content. Results showed that the optimizing parameters of spray-dried clam cooking liquor for powder were:feeding liquor temperature 60℃, inlet temperature 199℃, total solid content 30%,and atomization pressure 96 MPa. The dried powder of yield efficiency was 64.2% and water content was 3.9% under this spray- drying condition.
    Multi-residue determination method for the determination of brominated flame retardants in bivalves
    2017, 39(6):  476-484. 
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    A multi-residue method based on high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was developed for the quantitative determination of hexabromocyclododecane (HBCD) and tetrabromobisphenol A (TBBPA) in bivalves. The target analytes were extracted with the mixture of hexane and dichloromethane ( 1∶1, V/V). Then the co-extracted lipid was removed by sulfuric acid treatment.The newly obtained extract was cleaned up with silicone solid phase extraction column. The analytes were separated on a Thermo Hypersil Gold C18 column by gradient elution with water as mobile phase A and methanol-acetonitrile as mobile phase B, and detected by multiple reaction monitoring (MRM) with electrospray ionization (ESI) under negative ion mode. And the quantitative results were calculated by the internal standard method. The results showed that the calibration curves were linear (R2>0.995) in the concentration range of 0.5~100 ng/mL for HBCD and TBBPA. The limits of quantitation for HBCD and TBBPA were 0.05 μg/kg. The average recoveries at three spiked levels ranged from 70.5% to 92.4%. Intra-day and inter-day relative standard deviations (RSDs) were 3.4%-9.1% and 6.3%-10.2%, respectively.
    Development of desalination driven by green energy and a case of application in Pingtan Dayu Island of Fujian Province
    2017, 39(6):  485-492. 
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    This paper reviewed the development of desalination driven by green energy including wind energy and solar energy in the past 10~20 years while the desalination technology was dominated by the reverse osmosis ( RO) . Nowadays, the cost of green energy (0.05~0.09 $ /kWh) has been close to the cost of traditional fossil energy (0.05 ~ 0.09 $/kWh) , so the green energy driving desalination has the potential to solve the problems of traditional desalination technologies. Besides, this paper introduced a case study of wind/solar power driving RO desalination application in Pingtan Dayu Island of Fujian Province. The results of the project include: 1) successfully developing a wind/solar driving desalination system with a capacity of 10 m3/d , and optimizing the unstable energy generation and stable energy requirement via the energy storage using batteries; 2) determining the critical water production rate of 25% and maintained a low RO fouling level under no chemical usage conditions.
    Analysis and evaluation of water quality in Shishi inshore waters
    2017, 39(6):  493-501. 
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    The water quality in Shishi inshore was monitored from January to December in 2015. The physicochemical parameters including pH, dissolved oxygen, chemical oxygen demand, inorganic nitrogen, active phosphate and petroleum. The water quality was analyzed and evaluated by using single factor index method and eutrophication level evaluation method. The results showed that pH, dissolved oxygen, chemical oxygen demand of the water quality conformed to the corresponding environmental quality of marine functional zoning requirements, but inorganic nitrogen, phosphate and petroleum of water quality exceeded the standard in 2015.
    Development of the functional activity and amino acid composition of marine - derived bioactive peptides
    2017, 39(6):  502-508. 
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    Marine ecological system has the following characteristics : high pressure and high salt, low temperature and low light all the year round,less nutrition and more competition. So the marine organisms in their growth and metabolic process produce a large amount of materials with special chemical structure,special physiological activity and function. The activity of marine bioactive polypeptides,which have anti-hypertensive,anti-oxidation,anticoagulant and antibacterial activity,arouse great interest of scientific researchers . Currently,some of the polypeptides or analogs extracted from fish,shellfish and crustaceans have been listed or entered into clinical trials. This article reviewed the role of these bioactive peptides in functional foods, nutritional foods and drugs.