Table of Content

25 August 2015, Volume 37 Issue 4

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The morphology observation of the embryo and larva of Paphia undulata by scanning electron microscope

2015, 37(4):  263-269. 

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In view of the problems that the low success rate of artificial larval breeding and the lack of researches about early development of Paphia undulata，scanning electron microscopy was used to observe and describe the embryonic and larval development process of the clam，and the clear SEM photos showed the morphological changes of the early development were obtained. In Paphia undulata,the diameter of ovum wass 58.49μm, the shell length of D larvae and attached larvae wer 87.38μm and 200-220μm respectively.The morphological size was smaller than bivalve mollusk,such as Ruditapes philippinarum,Sinonovacula constricta,Mercenaria mercenaria,and so on.

Study on evolution of sediment after through engineering in Zhangzhou Bachimen seawall

2015, 37(4):  270-277. 

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Sea water exchange between Dongshan Bay and Zhao'an Bay is blocked by Bachimen seawall, where the seawater and environment of marine ecology is worse and worse. So Zhangzhou Municipal Government proposes to implement the Project of Excavation of Bachimen Seawall. This research calculated the scouring and silting law of Bachimen waterway before and after implementing the project of excavation by Jiaju Liu formula. Then the characteristics of Dongshan Bay and Zhao'an Bay hydrological dynamics were studied, based on which the effect of demolition and desilting to environment of Bachimen waterway was analysed. The results showed that hydrodynamic conditions would be changed in Bachimen waterway and its adjacent waters after demolition and desilting, where the projiect area would scour about 4 m depth, and the final depth would be up to 10.2 m, the both side of project will scour about 5~6 m depth, east and west sides of entrance would scour about 1~2 m depth, and erosion environment would become aggressive environment, which would change the status of ecological deterioration and promote the sustainable use of marine resources.

Variarion of chlorophyll a in Haitan Bay of Pingtan County, Fujian Province, China

2015, 37(4):  278-286. 

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The marine environmental status in Haitan Bay, Fujian Province, China, was monitored during June and November, 2013. The concentration of chlorophyll a (Chl a) was analyzed to assess its spatial and seasonal variability as well as the correlations with environmental and biological variables. The average Chl a concentration in Haitan Bay in spring and autumn were 3.1 mg/m3 and 1.1 mg/m3, respectively, higher than and approximately equal with the historical concentration of Chl a in the bays of northern Fujian and Taiwan Strait in spring and autumn, respectively. Chl a concentration decreased from inner Haitan bay to outer bay in spring and increased in autumn. The analysis showed good correlations between the concentration of Chl a with salinity, nutrients and density of zooplankton in spring, and with suspended solid in autumn.

Optimization conditions for growth of Platymonas subcordiformis for bait

2015, 37(4):  287-292. 

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In order to facilitate growing and promote productivity of microalgae—Platymonas subcordiformis with high nutritional value, optimization for growth conditions were investigated. A single factor experiment and L9 (34) orthogonal design method were conducted to study effects of illumination, temperature, nitrogen source and four nutrients. The results indicated the best growth conditions for Platymonas subcordiformis: intention of illumination was 3 000 Lx, temperature was 28℃, the optimum nitrogen source was urea concentration of 0.0 375 g/L, NaHCO3 0.09 g/L, FeCl3?6H2O 5 mg/L and KH2PO4 8.4 mg/L,respectively. Under the optimized conditions, Platymonas subcordiformis grew well and the biomass yield was 2.18 times compared with the conditions before optimigation.

The influence of different culture conditions and treatments on the cultivation effect of Saccharina japonica gametophytes

2015, 37(4):  293-300. 

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China has cultured the largest areas of kelp all over the world. It has firstly applied the gamete clone breeding techniques to the kelp hybridization practices in history. During the germplasm conservation and amplification cultivation of Saccharina gametes, bacterial contamination usually causes great losses to Saccharina germplasm resources and could further lead to economic losses to the kelp hybridization and kelp industry. In this study, four factors were set to test their effects on the growth and bacterial contamination of Saccharina japonica gametophytes. (1) Three means for flask sealing: Parafilm significantly inhibited the growth of the gametes (p<0.05) while filter paper and filter paper membrane did not. (2) Five means for seawater sterilization: Gamete clusters in boiled seawater and suction filtered boiled seawater groups reached the largest diameters which had significant preponderance over groups of double-antibody suction filtered boiled seawater, suction filtered unboiled seawater and double-antibody suction filtered unboiled seawater (p<0.05). (3) Two environments for illumination incubator: conventional laboratories and sterile room had no significant effect on the growth of the gametes. (4) Three environments for replacing culture medium: clean bench in both conventional laboratories and sterile room had no significant difference on the growth of gametes but had significant preponderance over experiment bench in conventional laboratories (p<0.05), and replacing the culture medium on experimental bench in conventional laboratories led to a bacterial contamination rate over 60%.

Effects of different feed additives on the growth and digestive enzyme activity of Babylonia lutosa

Xi-Fei CHEN

2015, 37(4):  301-307. 

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A feeding experiment was to determine the physiological effects of different feed additives on the growth and digestive enzymes include pepsase, amylase and lipase of Babylonia lutosa. Babylonia lutosa with average initial body mass of （3.99±0.94）g and shell height of （25.58±4.56） mm were divided into S0 group(the control group), S1 group (Chinese medicine group), S2 group (microbial ecological agent group), S3 group (multivitamin group) and S4 group (the mix group, chose one different agent every day). The results showed that: S4 had a highest average body mass by （11.79±0.32）g after experimenting for 40 d, which was 1.32 times higher than S0, different significantly. The digestive enzymes activity had a trend of increasing first and then decreasing, but there was synergy among the digestive enzymes. The effect of additive was more significant in S4, but there was a large fluctuations in S1 and S2, and kept stable in S3. So the experiment showed that S4 had a great effect on growth and digestive enzymes activity, but the effect weakened by the treating time, S1, S2 and S3 are also so. Especially, S1 had a high pepsase activity and S3 had a high lipase activity in the early experiment stage. It showed that low frequency additive adding could promote digestion performance and growth for Babylonia lutosa.

The effect of soaking time of extruded feeds and soft pellet diet for Pseudosciaena crocea on the swelling rate, the loss rate of dissolution and chemical oxygen demand (COD)

2015, 37(4):  308-313. 

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In the present study, a serial of Pseudosciaena crocea feeds were assessed, which mainly included seven different types of extruded feeds and five different types of soft pellet diet. The research detected the stability and effect on water quality of the feeds by measuring the swelling rate, the loss rate of dissolution and chemical oxygen demand (COD) after being immersed. The result showed that the swelling rate of the extruded feeds was significantly higher than the soft pellet diet (P<0.01),and the loss rate of dissolution of the extruded feeds was significantly lower than the soft pellet diet (P<0.01) after 1h. The COD of the extruded feeds was significantly higher than the soft pellet diet after 6h. There was significant positive correlation between the particle size and different time of two feeds and the swelling rate, the loss rate of dissolution and COD.

Primary research on Corbicula fluminea polysaccharides beverage technology

2015, 37(4):  314-319. 

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Primary research on Corbicula fluminea polysaccharides beverage polysaccharide technology was carried out by extracting polysaccharide in Corbicula fluminea through enzymatic hydrolysis by Chinese wolfberry extract. By considering the enzyme amount, enzymatic hydrolysis time, and material liquid ratio, orthogonal experiments [L9(34)] were carried out to obtain the optimize experiment conditions which the enzymatic hydrolysis time of 90 minutes, enzyme amount of 1.5%, material liquid ratio at 1:6, enzymatic hydrolysis temperature at 55℃, and pH of 7.5, and under which the yield of polysaccharide containing in Corbicula fluminea was 312.6 mg/100g. The best formula of Corbicula fluminea polysaccharides beverage, which was determined through orthogonal experiment method, were as follows: Corbicula fluminea polysaccharide extract（1 mg/ml）of 45 mL, Chinese wolfberry extract of 9 mL, and Spice extract of 0.3%. Used the sensory evaluation as comprehensive evaluation index, a Corbicula fluminea polysaccharides beverage was developed with natural taste and favor of Corbicula fluminea, fresh salty sweetness, refreshing and pleasant, as well as weak smell.

Research on the influence factors of offshore wind farm location in Fujian Province

2015, 37(4):  320-324. 

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Through summarizing the current situation and trend of offshore wind farm development, the paper made comprehensive and systematic analysis on the main influence factors of offshore wind farm location in Fujian Province by considering the domestic and foreign research results, and from the point of view of social, economic, natural, environmental and planning conditions. The paper provided an effective tool for offshore wind farm site selection and optimization, also provided reference for the examination and approval of using sea by marine management departments.

Advanced in bioactive sterols from marine resources

2015, 37(4):  325-337. 

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With marine species abundant, the sea offers enormous resources for structurally diverse natural compounds with various beneficial biological activities. Therefore, increasing knowledge on novel functional ingredients with biological activities from marine organisms has gained much attention. Marine organisms have proven to be a rich source of sterols. Additionally, sterols have been suggested to have anticancer, anti-inflammatory, antibacterial and antiviral activities. This article focuses on purification methods, biogenetic derivation, chemical construction and biological activity of sterols in order to provide reference for marine medicinal development.

Impacts of organic phosphorus pesticide in water environment for aquatic animals and human and its detection

2015, 37(4):  338-344. 

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Organic phosphorus pesticide (Ops), which has the characteristics of broad spectrum, high performance and more variety, is widely used in agriculture, forestry and fishery in our county after organochlorine pesticide. The abuse of Ops caused serious pollution to the water environment, which is becoming a threat to human, animal and aquatic environment. The toxicity mechanism, hazards to aquatic animal and human and the detection methods are introduced in this paper.