Table of Content

25 October 2012, Volume 34 Issue 5

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Genetic Relationships of Five Nile Tilapia Inferred from ISSR Analysis

2012, 34(5):  349-353. 

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ISSR analysis was conducted to investigate the genetic relationships of five Nile tilapia（population GF, NLⅠ, NLⅡ, NLⅢ and NLⅣ）. Out of 81 ISSR primers , 15 primers generated highly reproducible and polymorphic DNA fragments. The genetic trees were constructed based on ISSR amplification fingerprintings using unweighted pairgroup method for arithmetic averages analysis (UPGMA) methods. NLⅢ and NLⅣ strains formed one branch first, then grouped with GF population. NLⅠand NLⅡ formed a separate branch. This shows that: ①GF population separated from other Nile tilapia populations. ②The 500 bp band amplified by primer UBC835 could distinguish from GF and other Nile tilapia.

Cumulative Ecological Effects Assessment on Resource Exploitation Utilization in Bay based on interpretative structural modeling method

2012, 34(5):  354-361. 

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Base on analyzing main marine exploitation project in San-sha bay, 27 essential factors including topography and geomorphology ,water dynamical environment,water and sediment quality environment,ecological enviorment and the potential incurrence effect on disaster were selected from aspects of types of sea utilizing.Relations among these factors were analysed using interpretative structural modeling method(ISM) to set up the Interpretive Structural Modeling of cumulative ecological effects(CCE) on resources exploitation and utilization in Bay.The model clearly showed CCE occur process of the resource exploitation and utilization in Bay in every level.It offered the theoretical basis and technical method for the govenment planning and management in resources and environment.

The preliminary study on the classification method of shellfish-culture areas in Lianjiang southeast sea area, Fujian

2012, 34(5):  362-369. 

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Abstract: According to the data of environmental survey on harmful substances in seawater, surface sediments and cultured organisms from shellfish-culture areas of Lianjiang southeast sea area in April, June and August 2009, ecological environment quality of shellfish-culture areas was classified by the shellfish culture ecological environment quality synthesis assessment index method. The results showed that no area belonged to the first class, 46 areas with the area of 2978 hm2 (occupied 82.1% of all areas) belonged to the second class, and 10 areas with the area of 280 hm2 (occupied 17.9% of all areas) belonged to the third class in the 56 shellfish-culture areas of Lianjiang. To the second class, there were 14 areas belonged to 2a class, 24 areas belonged to 2b class and 8 areas belonged to 2c class. To the third class, there were 7 areas belonged to 3a class and 3 areas belonged to 3b class. The third class area was located in the Sinonovaculla Constricta beach culture areas of Aojiang estuary and Minjiang estuary, which corresponded with the investigation on the state of eco-environment in shellfish-culture areas of Lianjiang. compared between the synthesis assessment index method and the single parameter assessment method of escherichia coli quality. The results indicated that all the areas belong to the first class besides the mytilus edulis culture area belonging to the second class in the Houcaili Bay. These results have certain disparity with the classification by the synthesis assessment index method.

Determination of 3,6-anhydro galactose in agar and agarose

2012, 34(5):  370-374. 

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This paper studied the determination of resorcinol method on agar and agarose samples of 3,6-anhydro galactose and optimize the determination wavelength, color temperature, color time and colorimetric time. the results show that the optimum condition is determination of wavelength of 554nm,color temperature 80℃,reaction time 15min; under this condition, agar and agarose samples of 3,6-anhydro galactose were measured ,the relative deviation of 0.94% to 3.0%, and the spiked recovery of 105.4% to 111%. It shows that the method is simple, high accuracy and repeatability.

Determination of 13 sulfonamides in eels with solid phase extraction and high performance liquid chromatographic

2012, 34(5):  375-381. 

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A solid phase extraction and high performance liquid chromatographic method has been developed for the simultaneous determination of thirteen sulfonamides residues in eel products.The samples were extracted with ethyl acetate,concentrated and dissolved with hydrochloric acid ,then cleaned up by n-hexane liquid -liquid extraction and solid phase extraction.The residues were determined by high performance liquid chromatography with diode array detector and quantified by external standard method.The linear ranges were 0.01~2.0 mg/L with the correlation coefficient over 0.9999.The average recoveries in spiked samples were found to be in the range of 71.7%~89.8% with relative standard deviation of 0.80%~7.82%. The limits of detection were found to be 3.05 ~5.24 μg/kg and the limits of quantification 10.1 ~ 17.5 μg/kg.

The determination of elements Fe,Mn,Ni in the marine organisms by AAS

2012, 34(5):  382-386. 

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Distribution of Heavy Metals in the Surface Water of Luoyuan Bay

Ye-Fei Wu

2012, 34(5):  387-391. 

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Abstract: Based on the heavy metal data obtained from the surface water of Luoyuan Bay in August 2011, the heavy metal distribution characteristics and its influencing factor were analyzed. The results show that all heavy metal contents in surface seawaters were lower than the standard values of categories Ⅱ of the seawater quality standard; Cu、Pb and Cd tend to be gradually decreased from the coastal sea to the further offshore area, On the contrary, As and Hg are upward tendency;the heavy metals such as As、Hg are positively correlated with salinity, and Hg is negatively correlated with suspended materials, it is expounded that the formation of high value offshore areas is effected by the salinity and suspended matters; the correlations of Hg-As and Cd-Pb are obvious, which show that Hg-As and Cd-Pb have same source.

Primary analysis on red tide of Akashiwo sanguinea in winter Xiamen waters

2012, 34(5):  392-397. 

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According to the monitoring data of a Akashiwo sanguinea red tide occurred in Xiamen Sea in January and February 2009, the characteristic of red tide, and the relationships of environmental factors and density of Akashiwo sanguinea were analyzed. The results showed that the red tide event is the earliest and longest one in Xiamen sea. Akashiwo sanguinea was most dominant than Skeletonema costatum during the red tide and the process was not disturbed by the changes of water temperature. Higher salinity seems to have triggered the bloom. Besides, the occurrence of the red tide was unchecked by the low concentration phosphate and benefited by the high nitrogen.

The study on the temperature tolerance of red tilapia

2012, 34(5):  398-404. 

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In this paper, the temperature tolerance of red tilapia was studied at 20 ℃ as the initial temperature with gradually change of water temperature (increasing or decreasing 1 ℃ everyday, at the rate of 0.5 ℃ per 12hours ), and the suddenly increase (2 ℃, 4 ℃, 6 ℃, 8 ℃, 10 ℃, 12 ℃, 14 ℃, 16 ℃, 18 ℃, 20 ℃),and suddenly decrease ( 2 ℃, 4 ℃, 6 ℃, 8 ℃, 10 ℃, 12 ℃) ,respectively. In gradually increasing and decreasing water temperature experiments ,the results showed that the adaptation temperature of red tilapia was in the range from 8℃ to 40 ℃, and the critical survival temperature was from 14℃ to 36 ℃ .The death temperature , the lethal temperature, the critical lethal temperature and the fatal high temperature were 7.82 ± 0.21 ℃, 5.67 ± 0.16 ℃,38.36 ± 0.21 ℃,39.83 ± 0.15 ℃, ,respectively. With suddenly change of water temperature, the results showed that red tilapia was able to adapt the high temperature which it was able to adapt the suddenly increase change of 16 ℃(20℃→36℃), and only adapt the suddenly decrease change of 8 ℃(20℃→12℃) .

Mature acceleration and spawning inducement of parent oysterOf Amusium pleuronectes In Outdoor Tank

2012, 34(5):  405-409. 

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In this study, we developed the experiment of parent oysters which had different population density to promote maturity using outdoor concrete ponds. By intensive culture about 30 days, we found that 20-50 per cubic metre is better than others.Shady stimulation, temperature, algae solution, running water and opposite sex product were used to induce gametes releasing. The results indicates:shady stimulation 1 h + temperature stimulation + algae solution stimulation 1 h is better than other methods.

Characteristic of growth and feeding of Fugu flavidus in seawater ponds

2012, 34(5):  410-415. 

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Abstract: Fugu flavidus grows in early-stage faster than in late-stage in the culture ponds. It grows the faster in autumn than the other seasons. The optimum water temperature for growth is 23℃～29℃. The equation of power function between body length and body weight is W=0.05017L2.7866, R2=0.9739, which shows it grows in approximate equal speed. Illumination intensity of feeding peak is about 1900lux, it belongs to daytime fish closed to dawning and dusk. Fugu flavidus eats artificial feed very quickly from bottom to surface in short-time response and like clustering for food, which is convenient for feeding in fixed time and site.

Effects of concentrations of nitrogen and phosphorus and different culture methods on the growth of Caulerpa lentillifera

huang jianhui

2012, 34(5):  416-419. 

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Abstract: The effects of concentration of nitrogen and phosphorus and different culture methods on the growth of green macroalgae Caulerpa lentillifera were studied. Results showed that concentration of nitrogen had different effect on the growth of C. lentillifera. The wet weight of C. lentillifera was the highest when it was cultured in 15 mg?㎏-1 within the tested range of nitrogen concentration (0 mg?㎏-1~50 mg?㎏-). The effects of concentrations of phosphorus on the growth of C. lentillifera were similar. The wet weight of C. lentillifera was the highest in the 4 mg?㎏-1 in the tested treatments (0 mg?㎏-1~5 mg?㎏-1). Different culture methods had large influence on the growth of C. lentillifera. C. lentillifera was the best when it was cultured in basin among the flask, basin and nylon rope treatments.

The research process of fish genetic breeding

Jiang LiHua

2012, 34(5):  420-427. 

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Abstract: The paper reported the outlook of fish breeding, the first part was about traditional breeding methods of selective breeding, domestication and cross-breeding, secondly, modern biotechnology in fish breeding was also described, it included polyploidy breeding, androgenesis, nuclear transfer, transgenic, molecular marker assisted breeding and genome breeding. And the challenge and tendency were posed finally. Keywords: fish genetic breeding; research process

The application of oyster shell in water quality improvement

Yong-Chang SU wu chengye

2012, 34(5):  428-431. 

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Oyster shell has good capability of improving the water quality and it can effectively reduce a variety of pollutant in water. The main composition and structure of the oyster shell and the principle of improving the water quality were summarized. And the application status of dephosphorization, heavy metal removal, dyes removal and removing other pollutants were involved and the exploitation and application prospects were viewed.