关键词: 石莼, 功能性寡糖, 二次正交旋转, 优化, Ulva lactuca, functional oligosaccharide, the quadratic orthogonal rotational combination design, optimization

Abstract: Based on single factor experiments, the quadratic orthogonal rotational combination design was adopted to obtain the optimal processing conditions of ultrasonic - assisted cellulose extraction for Ulva lactuca functional oligosaccharide. Results showed that the optimal extraction processing conditions were cellulose dosage of 430 U/g, solid - liquid ratio of 1:30 (g/mL) , ultrasonic temperature of 50°C， ultrasonic time of 1.5 h，extract solution pH of 5. 0 and ultrasonic power of 450 W. Under the optimal extraction processing conditions，the extraction yield of Ulva lactuca soluble carbohydrate was 12. 02% . Then，with subsequent polysaccharides and protein removing operations， the extraction yield of Ulva lactuca functional was 2.38%.