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›› 2015, Vol. 37 ›› Issue (3): 175-181.

• 论文与报告 •    下一篇

几种诱食剂对克氏原螯虾生长、血液生化指标及消化酶活性的影响

万金娟1,沈美芳1,唐建清1,朱凛2,彭刚1,严维辉1,李佳佳1   

  1. 1. 江苏省淡水水产研究所
    2.
  • 收稿日期:2015-01-22 修回日期:2015-03-03 出版日期:2015-06-25 发布日期:2015-06-23
  • 通讯作者: 沈美芳
  • 基金资助:
    公益性行业(农业)科研专项基金项目;江苏省科技支撑计划项目(农业)项目

Effect of feeding attractants on growth performance, serum biochemical indexes and digestive enzyme activity of Procambarus clarkii

  • Received:2015-01-22 Revised:2015-03-03 Online:2015-06-25 Published:2015-06-23

摘要: 为研究饲料中添加诱食剂对克氏原螯虾生长、血液生化指标、肌肉常规营养成分及消化酶活性的影响,以初始体质量为(4.29 ±0.03)g 的克氏原螯虾(Procambarus clarkii) 为试验对象,选用克氏原螯虾231只,随机分为7个处理组,每个处理设3 个重复,每组重复11 只。采用单因素试验设计,在基础饲料中分别添加1% 甜菜碱、TMAO、DMPT、复合氨基酸、大蒜素和复合诱食剂(40% 甜菜碱+20% TMAO + 20% DMPT + 20% 大蒜素),试验结束后测定克氏原螯虾生长性能、血清生化指标、肌肉常规营养成分和消化酶活性。结果显示,与对照组相比,在饲料中添加甜菜碱、TMAO、复合氨基酸、大蒜素、复合诱食剂均能显著提高克氏原螯虾的增重率,降低饲料系数(P < 0.05),复合诱食剂还可以显著提高其摄食率(P < 0.05);而对存活率没有显著影响(P > 0.05)。DMPT、复合氨基酸和复合诱食剂组均能显著降低血清谷丙转氨酶的水平;复合氨基酸组还能显著降低胆固醇的水平(P<0.05)。添加甜菜碱、TMAO、DMPT、大蒜素和复合诱食剂组肌肉粗脂肪含量显著高于对照组(P <0.05)。此外,在日粮中添加诱食剂能提高虾肠道消化酶活性(P < 0.05)。各组间血清谷草转氨酶、葡萄糖、总蛋白和白蛋白水平、肌肉水分和灰分含量差异均不显著(P>0.05)。综上所述,饲料中添加诱食剂能显著提高克氏原螯虾的摄食、生长以及消化酶的活性。其中,复合诱食剂(40% 甜菜碱+ 20% TMAO + 20% DMPT + 20% 大蒜素) 对促进克氏原螯虾生长效果最显著。

关键词: 诱食剂, 克氏原螯虾, 生长, 血清生化指标, 消化酶, 肌肉常规营养成分, Procambarus Clarkii, feeding attractants, growth, serum biochemical indexes, digestive enzyme activity, muscle composition

Abstract: The experiment was conducted to determine the effect of dietary containing 1% betaine, TMAO, DMPT, compound amino-acid , allicin and compound feeding attractant (40% bataine + 20% TMAO + 20% DMPT + 20% allicin) on growth performance, serum biochemical indexes, muscle composition and digestive enzyme activity of Procambarus clarkii (4.29±0.03 g). The results indicated that compared with control group, the weight gain rate (WGR), feeding rate (FR) were significantly increased and feed conversion rate (FCR) were significantly decreased in group of 1% bataine, allicin and compound feeding attractant (P<0.05); there was no significant difference of survival rate among the groups (P>0.05). Serum alanine aminotransferase (ALT) level was significantly lower in 1% DMPT, compound amino-acid and compound feeding attractant than that of control group; and the content of serum total cholesterol (TCH) was the lowest in 1% compound amino-acid (P<0.05). Moreover, muscle crude lipid content was significantly lower in control group and 1% compound amino-acid than other groups (P<0.05). The activity of digestive enzyme was significantly higher in experimental treatments (P<0.05). Additionally, there were no significantly differences in levels of glutamic-oxaloacetic transaminase (AST), glucose (Glu), total protein (TP), muscle moisture and ash among the groups (P>0.05). In conclusion, adding feeding attractants could improve the growth performance and digestive enzyme activity of Procambarus Clarkii, especially for compound feeding attractant.

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