关键词: 海螵蛸, 凝血时间, 出血时间, 凝血机制, cuttlebone, clotting time, bleeding time, clotting mechanism

Abstract: With modern means of pharmacology, Kunming mice were fed with different doses of cuttlebone solution and cuttlebone which has different content of calcium. Through the determination of Kunming mice’s clotting time and bleeding time, verify the hemostatic effect of cuttlebone. By comparing the different groups’ bleeding time and clotting time, the paper discussed the possible clotting mechanism. The results showed that under the 250 g / L dose, cuttlebone group’s bleeding time and clotting time were shorter 11.45 min and 1.38 min than the saline control group, respectively. The average bleeding time and clotting time of cuttlebone was 3.48 min and 0.49 min. Compared with normal saline control group, bleeding and clotting time of cuttlebone solid group with different degrees of decalcification were significant difference which showed that they also had clotting activity and the cuttlebone without decalcified had the shortest clotting time and best coagulation effect. Therefore, cuttlebone not only has clotting activity but also has a dose-dependent manner.