关键词: 后海, 牡蛎, 形态特征, 分子鉴定, 同工酶, Houhai bay, oyster, morphological characteristics, molecular identification, isozymes

Abstract: We observed the morphological characteristics and measured the key measurable character indicators of the common oyster cultured in the Houhai bay. The 16S rRNA and CO I were sequenced and the phylogenetic trees involved in seven species of oysters were reconstructed based on the sequence similarities and genetic distances of 16S rRNA and CO I gene sequences in order to identify the isolated samples. Also, the electrophoretograms of isozymes (IDH, SOD) from the digestive gland tissue were analyzed. The results showed that the oyster’s shell shape were different from each other because of the living environment. The oyster from the Houhai bay shoud not be classified as Saccostrea cucullata but Crassostrea angulata. IDH and SOD were found expressing in the digestive gland tissue but no polymorphic loci was detected. The combination of morphological characteristics and molecular phylogenetic analysis has provided a good method to identify the oyster.