斑鳢Channa. maculata（♀）和乌鳢C.argus(♂)杂交子代及其亲本的遗传关系分析与鉴定

福建水产 ›› 2013, Vol. 35 ›› Issue (2): 93-99.

斑鳢Channa. maculata（♀）和乌鳢C.argus(♂)杂交子代及其亲本的遗传关系分析与鉴定

江小斌

福州市海洋与渔业技术中心

收稿日期:2013-01-24 修回日期:2013-02-17 出版日期:2013-04-25 发布日期:2013-04-27
通讯作者: 江小斌
基金资助:
省科技厅重点科技攻关项目

Genetic biology analysis and identification of F1 hybrids[ Channa argus(♂)×C. maculata（♀）]

Received:2013-01-24 Revised:2013-02-17 Online:2013-04-25 Published:2013-04-27

摘要/Abstract

摘要： 应用RAPD技术研究闽香鳢及其亲本之间在遗传结构、种群多样性上的相互关系，由此为探讨种质鉴定提供依据。在所用的90个随机引物中，有35个引物在闽香鳢及其亲本之间呈现多态。通过RAPD分析，获取了闽香鳢及其亲本的RAPD指纹图带。结果表明闽香鳢与斑鳢或乌鳢的遗传距离要比其父母本之间的遗传距离近，即闽香鳢为父母本杂交所产生的后代。闽香鳢种群的多态性比例47.9%与杂合度H=0.122,可作为闽香鳢遗传育种的指标来检测后代的群体遗传多样性。通过多态性比较，获得2条具有群体差异性的DNA片断，大小分别为910 bp和1011 bp，经过测序后合成特异性引物，此引物可特异性区分上述3种鱼。

关键词: 鳢属, 杂交育种, 遗传结构, RAPD, 指纹图谱, 特异性标记

Abstract: Abstract: Random amplified polymorphic DNA (RAPD) technique was applied to assess the relationship of genetic structure and species diversity between hybrids F1 and their parents. The RAPD fingerprints of hybrids F1 and their parents were obtained with RAPD analysis using 90 random primers, of which 35 generated polymorphic products. The results show that the genetic distance between hybrids F1 and Channa argus（♀）or C. maculata(♂) was closer than between their parents, it meant hybrids F1 were the hybrid generation of their parents.The polymorphic proportion of F1 hybrids 47.9% and hybridzation degree H=0.122 could be the genetic breeding standard to measure the genetic diversity of the species progeny.

江小斌. 斑鳢Channa. maculata（♀）和乌鳢C.argus(♂)杂交子代及其亲本的遗传关系分析与鉴定[J]. 福建水产, 2013, 35(2): 93-99.