盐度胁迫对褐菖鲉幼鱼消化酶和非特异性免疫酶的影响

doi:10.14012/j.cnki.fjsc.2024.02.003

渔业研究 ›› 2024, Vol. 46 ›› Issue (2): 129-135.DOI: 10.14012/j.cnki.fjsc.2024.02.003

盐度胁迫对褐菖鲉幼鱼消化酶和非特异性免疫酶的影响

蒋飞¹^,²(), 徐嘉波¹, 林琪²^,³, 施永海¹^,^*(), 贾垂攀¹, 严银龙¹, 邓平平¹, 于爱清¹, 张之文¹, 袁新程¹

1.上海市水产研究所,上海市水产技术推广站,上海 200433
2.福建省海洋生物增养殖与高值化利用重点实验室,福建厦门 361013
3.福建省水产研究所,福建厦门 361013

收稿日期:2023-08-01 出版日期:2024-04-25 发布日期:2024-04-25
通讯作者: 施永海(1975—),男,研究员,研究方向:水产养殖、水环境监测及繁殖生物学。E-mail:yonghais@163.com
作者简介:蒋飞(1989—),男,高级工程师,硕士,研究方向:水产养殖及育种。E-mail:ygljf214@163.com
基金资助:
福建省海洋生物增养殖与高值化利用重点实验室开放课题项目(2021fjscq02);上海领军人才项目(沪人社专2021-67号);上海长江口主要经济水生动物人工繁育工程技术研究中心(13DZ2251800)

Effects of salinity on digestive enzyme and non-specific immune enzyme of juvenile Sebastiscus marmoratus

JIANG Fei¹^,²(), XU Jiabo¹, LIN Qi²^,³, SHI Yonghai¹^,^*(), JIA Chuipan¹, YAN Yinlong¹, DENG Pingping¹, YU Aiqing¹, ZHANG Zhiwen¹, YUAN Xincheng¹

1. Shanghai Fisheries Research Institute,Shanghai Fisheries Technical Extension Station,;Shanghai 200433, China
2. Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian Province, Xiamen 361013, China
3. Fisheries Research Institute of Fujian, Xiamen 361013, China

Received:2023-08-01 Online:2024-04-25 Published:2024-04-25

摘要/Abstract

摘要：

为探讨褐菖鲉(Sebastiscus marmoratus)幼鱼适应盐度骤变的能力,本文以肠胃消化酶和肝脏非特异性免疫酶活性为指标,研究其在盐度6、8、10和20[分别记为S6、S8、S10和S20(对照)]胁迫下的变化规律。结果显示,当盐度胁迫96 h时,S10和S20组的胰蛋白酶、胃蛋白酶、淀粉酶和脂肪酶活性均显著高于S6和S8组(P<0.05);S6和S8组谷丙转氨酶和谷草转氨酶活性均显著高于S10和S20组(P<0.05);S6和S8组酸性磷酸酶和碱性磷酸酶活性均无显著性差异(P>0.05);S10和S20组酸性磷酸酶和碱性磷酸酶活性均呈先上升后下降趋势,均在12 h时达到峰值,且显著高于其他组。研究结果表明,褐菖鲉幼鱼适宜的养殖盐度为10~20。

关键词: 褐菖鲉, 盐度胁迫, 消化酶, 非特异性免疫酶

Abstract:

To investigate the changes in digestive enzyme activities in the intestine and stomach, as well as the activities of non-specific immunoenzymes in the liver of juvenile Sebastiscus marmoratus under salinity stress, and to assess the ability of the juveniles to adapt to sudden changes in salinity, a salinity experiment was conducted. The experiment comprised four groups, representing different salinity gradient levels: 6, 8, 10, and 20 (control). These groups were referred to as S6, S8, S10, and S20, respectively. Results indicated that at the acute stress period of 96 hours, the activities of trypsin, pepsin, amylase, and lipase in S10 and S20 were significantly higher compared to those in S6 and S8 (P<0.05). Conversely, the activities of glutamic-pyruvic transaminase and glutamic-oxaloacetic transaminase were significantly higher in S6 and S8 compared to S10 and S20 (P<0.05). There was no significant difference in the activities of ACP and AKP between S6 and S8 (P>0.05). Additionally, the activities of ACP and AKP increased initially and then decreased over time in S10 and S20. These activities in S10 and S20 peaked at 12 hours, signifying a significant increase compared to other salinity levels. These findings suggest that the optimal salinity for breeding juvenile S.marmoratus ranges from 10 to 20.

Key words: Sebastiscus marmoratus, salinity stress, digestive enzyme, non-specific immune enzyme

中图分类号:

S968.1

蒋飞, 徐嘉波, 林琪, 施永海, 贾垂攀, 严银龙, 邓平平, 于爱清, 张之文, 袁新程. 盐度胁迫对褐菖鲉幼鱼消化酶和非特异性免疫酶的影响[J]. 渔业研究, 2024, 46(2): 129-135.

JIANG Fei, XU Jiabo, LIN Qi, SHI Yonghai, JIA Chuipan, YAN Yinlong, DENG Pingping, YU Aiqing, ZHANG Zhiwen, YUAN Xincheng. Effects of salinity on digestive enzyme and non-specific immune enzyme of juvenile Sebastiscus marmoratus[J]. Journal of Fisheries Research, 2024, 46(2): 129-135.

图/表 8

图1 不同盐度下褐菖鲉幼鱼胃肠胰蛋白酶活性注:不同小写字母表示不同盐度组之间存在显著差异(P<0.05);相同字母表示无显著差异(P>0.05)。图2~图6同此。

Fig.1 The trypsin activity in the intestine and stomach of juvenile S. marmoratus at the different salinity level Notes: Different small letters indicated significant differences at the different salinity level (P<0.05); the same small letter indicated no significant difference (P>0.05).It’s the same as in Fig.2-Fig.6.

图2 不同盐度下褐菖鲉幼鱼胃肠淀粉酶活性

Fig.2 The amylase activity in the intestine and stomach of juvenile S. marmoratus at the different salinity level

图3 不同盐度下褐菖鲉幼鱼胃肠胃蛋白酶活性

Fig.3 The pepsin activity in the intestine and stomach of juvenile S. marmoratus at the different salinity level

图4 不同盐度下褐菖鲉幼鱼胃肠脂肪酶活性

Fig.4 The lipase activity in the intestine and stomach of juvenile S. marmoratus at the different salinity level

图5 不同盐度下褐菖鲉幼鱼谷丙转氨酶活性

Fig.5 The glutamic-pyruvic transaminase activity in liver of juvenile S. marmoratus at the different salinity level

图6 不同盐度下褐菖鲉幼鱼谷草转氨酶活性

Fig.6 The glutamic-oxaloacetic transaminase activity in liver of juvenile S. marmoratus at the different salinity level

图7 盐度胁迫对褐菖鲉幼鱼肝酸性磷酸酶活性的影响注:不同大写字母表示同一盐度不同时间点之间存在显著差异(P<0.05);不同小写字母表示同一时间点不同盐度组之间存在显著差异(P<0.05);相同字母或未标注字母的表示无显著差异(P>0.05)。图8同此。

Fig.7 Effects of low salinity on the liver ACP activity of juvenile S. marmoratus Notes: Different capital letters indicated significant differences in the same salinity level but with different time(P<0.05); different small letters indicated significant differences in the same time but with different salinity levels(P<0.05); the same letters or unlettered letters indicated no significant difference (P>0.05). It’s the same as in Fig.8.

图8 盐度胁迫对褐菖鲉幼鱼肝碱性磷酸酶活性的影响

Fig.8 Effects of low salinity on the liver AKP activity of juvenile S. marmoratus

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盐度胁迫对褐菖鲉幼鱼消化酶和非特异性免疫酶的影响

Effects of salinity on digestive enzyme and non-specific immune enzyme of juvenile Sebastiscus marmoratus

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