饲料蛋白质水平对杂交黄颡鱼生长、消化酶活性和抗氧化能力的影响

doi:10.14012/j.cnki.fjsc.2023.03.008

渔业研究 ›› 2023, Vol. 45 ›› Issue (3): 280-286.DOI: 10.14012/j.cnki.fjsc.2023.03.008

饲料蛋白质水平对杂交黄颡鱼生长、消化酶活性和抗氧化能力的影响

唐东姣¹(), 冯鹏霏², 黄亮华³, 何金钊⁴, 秦秀东⁵, 马华威², 陈子桂⁴, 李恩军⁵, 易祖晓⁵, 陈少翠², 秦荣娟⁵, 徐佳智⁵, 潘传燕²^,^*()

1.全州县全州镇水产畜牧兽医站,广西桂林 541500
2.广西壮族自治区水产科学研究院,广西水产遗传育种与健康养殖重点实验室,广西南宁 530021
3.广西中医药大学海洋药物研究院,广西南宁 530200
4.广西壮族自治区水产引育种中心,广西南宁 530031
5.桂林市漓江补水枢纽工程渔业增殖管理站,广西桂林 541006

收稿日期:2022-12-07 出版日期:2023-06-25 发布日期:2023-06-16
通讯作者: 潘传燕
作者简介:唐东姣(1976—),女,工程师,研究方向为水产动物营养与饲料。E-mail:3471874971@qq.com
基金资助:
2022年广西农业科技自筹经费项目(Z202283);2022年广西农业科技自筹经费项目(Z2022213)

Effects of dietary protein levels on growth,digestive enzymes activities and antioxidant ability of hybrid yellow catfish

TANG Dongjiao¹(), FENG Pengfei², HAUNG Lianghua³, HE Jinzhao⁴, QIN Xiudong⁵, MA Huawei², CHEN Zigui⁴, LI Enjun⁵, YI Zuxiao⁵, CHEN Shaocui², QIN Rongjuan⁵, XU Jiazhi⁵, PAN Chuanyan²^,^*()

1. Aquatic Animal Husbandry Veterinary Station of Quanzhou County,Guilin 541500,China
2. Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Sciences,Nanning 530021,China
3. Institute of Marine Drugs,Guangxi University of Chinese Medicine,Nanning 530200,China
4. Guangxi Aquatic Species Introduction and Breeding Center,Nanning 530031,China
5. Lijiang River Water Replenishment Hub Project and Fishing and Breeding Management Station,Guilin 541006,China

Received:2022-12-07 Online:2023-06-25 Published:2023-06-16
Contact: PAN Chuanyan

摘要/Abstract

摘要：

为探讨饲料蛋白质水平对杂交黄颡鱼(黄颡鱼♀×瓦氏黄颡鱼♂)生长、消化酶活性和抗氧化能力的影响,制备蛋白质水平分别为30%、35%、40%、45%和50%的5种试验饲料并用其喂养(1.00 ± 0.05)g杂交黄颡鱼,投喂试验时间为60 d,以确定杂交黄颡鱼饲料蛋白质的最适添加水平。结果表明:1)当饲料蛋白质水平为40%~45%时,杂交黄颡鱼生长性能最佳,杂交黄颡鱼的增重率、特定生长率等生长指标均显著提高(P<0.05)。2)饲料蛋白质水平40%组的杂交黄颡鱼的胃和肠道蛋白酶活性最高(P<0.05),饲料蛋白质水平45%组的淀粉酶和脂肪酶活性最高(P<0.05);各组胃蛋白酶活性均高于肠道,肠道淀粉酶、脂肪酶活性在饲料蛋白质水平≥40%时高于胃。3)饲料蛋白质水平为40%~45%组的杂交黄颡鱼肝脏超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GPX)活性显著高于其他组(P<0.05);当饲料蛋白质水平≥40%时,过氧化氢酶(CAT)活性较高(P<0.05);丙二醛(MDA)含量随饲料蛋白质水平的增加呈先下降后上升的趋势。综上所述,(1.00 ± 0.05)g杂交黄颡鱼饲料中蛋白质的最适添加水平为40%~45%。

关键词: 杂交黄颡鱼, 蛋白质水平, 生长性能, 消化酶, 抗氧化能力

Abstract:

The study was conducted to explore the effects of dietary protein levels on growth,digestive enzymes activities and antioxidant ability of hybrid yellow catfish (Pelteobagrus fulvidraco♀×P.vachelli♂).Five experimental diets were formulated containing dietary protein 30%,35%,40%,45% and 50%,respectively.Hybrid yellow catfish [(1.00±0.05)g] were fed with five experimental diets for 60 days.The objective of this experiment was to determine the dietary protein requirement of hybrid yellow catfish.The results indicated that the dietary protein level was 40%-45%,the growth performance of hybrid yellow catfish was the best,and the final weight,specific growth rate (SGR) were increased significantly (P<0.05).The activity of protease in stomach and intestine in 40% protein group was the highest,the activity of lipase and amylase in 45% protein group was the highest.In all groups,the activity of protease in stomach was higher than that of intestine.The activities of lipase and amylase in intestine were higher than that of stomach when the dietary protein level exceeded 40% (including 40%).The activities of superoxide dismutase (SOD) and glutathione peroxidase (GPX) in the liver of hybrid yellow catfish were significantly higher than that of other groups (P<0.05) when the dietary protein level was 40%-45%.The catalase (CAT) activities were higher than those of other groups when the dietary protein level exceeded 40% (including 40%).The content of malonaldehyde (MDA) decreased and then increased with the increase of dietary protein level.In conclusion,the optimal dietary protein level of hybrid yellow catfish [(1.00±0.05)g] was 40%-45%.

Key words: hybrid yellow catfish (Pelteobagrus fulvidraco♀×P.vachelli♂);, protein level, growth performance, digestive enzyme, antioxidant ability

中图分类号:

S964.3

唐东姣, 冯鹏霏, 黄亮华, 何金钊, 秦秀东, 马华威, 陈子桂, 李恩军, 易祖晓, 陈少翠, 秦荣娟, 徐佳智, 潘传燕. 饲料蛋白质水平对杂交黄颡鱼生长、消化酶活性和抗氧化能力的影响[J]. 渔业研究, 2023, 45(3): 280-286.

TANG Dongjiao, FENG Pengfei, HAUNG Lianghua, HE Jinzhao, QIN Xiudong, MA Huawei, CHEN Zigui, LI Enjun, YI Zuxiao, CHEN Shaocui, QIN Rongjuan, XU Jiazhi, PAN Chuanyan. Effects of dietary protein levels on growth,digestive enzymes activities and antioxidant ability of hybrid yellow catfish[J]. Journal of Fisheries Research, 2023, 45(3): 280-286.

图/表 4

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主要化学成分 Chemical composition	试验饲料Experimental diets
主要化学成分 Chemical composition	P30	P35	P40	P45	P50
粗蛋白质/% Crude protein	30.32	34.87	40.06	45.65	50.26
粗脂肪/% Crude lipid	7.81	7.83	7.83	7.84	7.83
灰分/% Ash	8.05	8.56	8.62	9.03	9.12
能量/(kJ ·g^-1) Energy	16.84	17.12	17.86	17.94	17.90

主要化学成分 Chemical composition	试验饲料Experimental diets
主要化学成分 Chemical composition	P30	P35	P40	P45	P50
粗蛋白质/% Crude protein	30.32	34.87	40.06	45.65	50.26
粗脂肪/% Crude lipid	7.81	7.83	7.83	7.84	7.83
灰分/% Ash	8.05	8.56	8.62	9.03	9.12
能量/(kJ ·g^-1) Energy	16.84	17.12	17.86	17.94	17.90

指标Index	试验饲料Experimental diets
指标Index	P30	P35	P40	P45	P50
存活率SR	95.68±1.05	94.25±1.84	95.03±2.01	92.23±1.52	94.42±1.21
增重率WGR	488.67±25.80^d	518.74±36.02^c	585.32±22.45^a	586.14±42.87^a	550.84±19.34^b
特定生长率SGR	2.87±0.12^c	3.01±0.08^b	3.24±0.05^a	3.18±0.10^ab	3.08±0.07^b
饲料系数FCR	2.93±0.24^a	2.68±0.14^b	2.43±0.20^c	2.46±0.14^c	2.62±0.22^b

指标Index	试验饲料Experimental diets
指标Index	P30	P35	P40	P45	P50
存活率SR	95.68±1.05	94.25±1.84	95.03±2.01	92.23±1.52	94.42±1.21
增重率WGR	488.67±25.80^d	518.74±36.02^c	585.32±22.45^a	586.14±42.87^a	550.84±19.34^b
特定生长率SGR	2.87±0.12^c	3.01±0.08^b	3.24±0.05^a	3.18±0.10^ab	3.08±0.07^b
饲料系数FCR	2.93±0.24^a	2.68±0.14^b	2.43±0.20^c	2.46±0.14^c	2.62±0.22^b

消化系统 Digestive system	指标 Index	试验饲料Experimental diets
消化系统 Digestive system	指标 Index	P30	P35	P40	P45	P50
肠道 Intestinal	蛋白酶/ U·mg^-1	24.15±2.15^c	36.21±1.88^b	40.26±3.02^a	37.54±2.11^b	35.46±1.54^bc
	淀粉酶/U·mg^-1	1.42±0.24^c	1.48±0.13^c	1.98±0.40^b	2.42±0.32^a	2.02±0.21^b
	脂肪酶/U·g^-1	32.50±2.45^d	36.26±1.98^c	41.23±4.05^a	42.01±2.66^a	39.74±1.68^ab
胃 Stomach	蛋白酶/ U·mg^-1	34.64±3.84^cd	40.25±2.32^b	50.20±5.07^a	39.85±2.87^b	37.68±3.65^bc
	淀粉酶/U·mg^-1	1.75±0.56	1.79±0.19	1.82±0.35	1.84±0.14	1.80±0.26
	脂肪酶/ U·g^-1	34.55±1.88^b	34.36±4.29^b	35.86±2.50^a	36.12±4.06^a	35.47±2.34^a

饲料蛋白质水平对杂交黄颡鱼生长、消化酶活性和抗氧化能力的影响

Effects of dietary protein levels on growth,digestive enzymes activities and antioxidant ability of hybrid yellow catfish

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Metrics

抗氧化酶 Antioxidase	试验饲料Experimental diets
抗氧化酶 Antioxidase	P30	P35	P40	P45	P50
超氧化物歧化酶/U·mL^-1 SOD	10.68±0.54^c	12.05±0.84^b	13.67±0.62^a	13.85±0.24^a	12.88±0.49^ab
过氧化氢酶/U·mL^-1 CAT	1.31±0.08^c	1.26±0.12^c	1.56±0.06^a	1.44±0.17^b	1.46±0.20^b
谷胱甘肽过氧化物酶/U·mL^-1 GPX	241.48±10.20^c	259.22±8.56^b	280.32±6.87^a	274.28±12.45^a	261.44±9.23^b
丙二醛/nmol·mL^-1MDA	2.20±0.19^a	2.14±0.15^a	1.33±0.12^c	1.46±0.09^c	1.96±0.22^b

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