呋喃西林代谢物氨基脲在菊黄东方鲀肌肉中的残留消除规律研究

doi:10.14012/j.cnki.fjsc.2023.05.009

摘要/Abstract

摘要：

为了解菊黄东方鲀使用呋喃西林药物后的代谢物氨基脲(Semicarbazide,SEM)在其肌肉中的残留及消除规律,根据菊黄东方鲀(Takifugu flavidus)养殖的实际情况,以剂量为0.50 g/m³的呋喃西林原粉对养殖池进行全池泼洒,定期随机采集菊黄东方鲀肌肉样品,利用高效液相色谱-串联质谱法测定肌肉中的SEM质量浓度。结果表明,给药后菊黄东方鲀肌肉中SEM含量逐渐升高,约4 d后达到峰值,随后逐渐消除,消除速率在初始阶段较快,之后趋势缓慢,并在较长时间内维持一定的浓度范围,直至77 d实验结束时仍检出约2.50 μg/kg的SEM残留量。使用呋喃西林后的代谢物SEM在菊黄东方鲀肌肉中的残留时间较长,因此在养殖过程中,应严格禁止硝基呋喃药物的使用,从而保证河鲀鱼的质量安全。

关键词: 呋喃西林代谢物, 菊黄东方鲀, 残留消除规律, 高效液相色谱-串联质谱法

Abstract:

In order to investigate the retention and elimination of furacillin metabolites (semicarbazide,SEM) in the muscle of T.flavidus following the use of furacilin during the breeding process,the aquaculture pond was treated with 0.50 g/m³ of furacillin.Muscle samples of T.flavidus were then collected at regular intervals.The concentrations of SEM were determined using high-performance liquid chromatography-tandem mass spectrometry,and the data was analyzed by using Excel 2013.The results showed that furacillin was rapidly metabolized to SEM in T.flavidus after dosing.The levels of SEM in the muscles increased rapidly,reaching their highest value after approximately 4 days.The elimination rate of SEM was initially fast,but slowed down over time.Even after 77 days following dosing,the residual amount of SEM in the muscles was still around 2.50 μg/kg,which was above the limit of detection.This suggests that SEM has a prolonged residual time in T.flavidus after the administration of furacilin.Based on these findings,it is strongly recommended to strictly prohibit the use of nitrofurans,such as furacilin,during the breeding process of T.flavidus,in order to ensure the quality and safety of puffer fish.

Key words: furacillin metabolites, Takifugu flavidus, retention and elimination, high performance liquid chromatography-tandem mass spectrometry

中图分类号:

O656.31

汤水粉, 钱卓真, 李雷斌, 刘智禹, 王丽娟, 位绍红, 姜琳琳, 余颖, 陈宇锋, 刘海新, 陈思, 罗方方. 呋喃西林代谢物氨基脲在菊黄东方鲀肌肉中的残留消除规律研究[J]. 渔业研究, 2023, 45(5): 490-495.

TANG Shuifen, QIAN Zhuozhen, LI Leibin, LIU Zhiyu, WANG Lijuan, WEI Shaohong, JIANG Linlin, YU Ying, CHEN Yufeng, LIU Haixin, CHEN Si, LUO Fangfang. Study on the residue and elimination of furacillin metabolites (semicarbazide) in the muscle of Takifugu flavidus[J]. Journal of Fisheries Research, 2023, 45(5): 490-495.

图/表 4

表1 流动相梯度洗脱程序

Tab.1 Gradient elution program

时间/min Time	A/%	B/%
0	80.0	20.0
1.00	80.0	20.0
2.00	10.0	90.0
7.00	10.0	90.0
7.50	80.0	20.0
12.50	80.0	20.0

图1 SEM 标准品衍生产物选择离子流图

Fig.1 Selected reaction monitoring chromatograms of the derivative of SEM standard

图2 鱼肉空白样品(A)和加标样(B)选择离子流图

Fig.2 Selected reaction monitoring chromatograms of blank fishes sample (A) and spiked fishes sample (B)

图3 菊黄东方鲀肌肉中SEM药时曲线

Fig.3 The curve of SEM concentrations-time in the muscle of T. flavidus

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