罗氏沼虾3个不同群体线粒体COⅠ基因序列变异及遗传多样性分析

doi:10.14012/j.cnki.fjsc.2023.01.002

渔业研究 ›› 2023, Vol. 45 ›› Issue (1): 8-13.DOI: 10.14012/j.cnki.fjsc.2023.01.002

罗氏沼虾3个不同群体线粒体COⅠ基因序列变异及遗传多样性分析

蒋飞¹(), 戴习林²^,^*()

1.上海市水产研究所,上海市水产技术推广站,上海 200433
2.上海海洋大学,水产科学国家级实验教学示范中心,农业部淡水水产种质资源重点实验室,水产动物遗传育种中心上海市协同创新中心,上海 201306

收稿日期:2022-06-20 出版日期:2023-02-25 发布日期:2023-02-21
通讯作者: 戴习林(1969-),男,教授,研究方向:水产养殖及育种研究。E-mail:xldai@shou.edu.cn
作者简介:蒋飞(1989-),男,工程师,硕士,研究方向:水产养殖及育种研究。E-mail:ygljf214@163.com
基金资助:
上海市虾类产业技术体系建设项目(沪农科产〔2014〕第5号)

Analysis of the genetic diversity and sequence variation of mitochondrial COⅠgene from three populations of Macrobrachium rosenbergii

JIANG Fei¹(), DAI Xilin²^,^*()

1. Shanghai Fisheries Research Institute, Shanghai Fisheries Technical Extension Station, Shanghai 200433, China
2. National Demonstration Center for Experimental Fisheries Science Education, Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Collaborative Innovation of Aquatic Animal Genetics and Breeding, Shanghai Ocean University, Shanghai 201306, China

Received:2022-06-20 Online:2023-02-25 Published:2023-02-21

摘要/Abstract

摘要：

为研究上海选育群体(SC)、浙江养殖群体(ZJ)和泰国野生群体(TL)3个罗氏沼虾群体的遗传多样性,实验对3个不同群体的线粒体CO Ⅰ 基因序列进行PCR扩增和测定。在长度为458 bp的CO Ⅰ 基因序列中,只检测到8个变异位点和4个单倍型;平均A+T含量(54.4%)高于G+C含量(45.7%)。遗传变异参数分析显示,单倍型多样性指数最高为TL群体(Hd=0.733),核苷酸多样性指数最高为ZJ群体(π=0.005)。AMOVA分析表明,来自群体间的遗传变异(74.5%)高于来自群体内的遗传变异(25.5%)。采用NJ法和UPMGA法构建的系统进化树的趋势一致,都显示SC群体和ZJ群体遗传关系最近,首先聚在一起成为一小支,然后再与TL群体聚为另一支。研究结果可为罗氏沼虾种质资源保护和利用提供参考。

关键词: 罗氏沼虾, COⅠ基因, 遗传多样性, 遗传分化

Abstract:

In order to investigate the genetic diversity among three populations of Macrobrachium rosenbergii collected from Shanghai breeding population (SC), Zhejiang cultured population (ZJ) and Thailand wild population (TL), the mitochondrial sequences of the COⅠ gene in the three populations were amplified and determined by PCR and sequencing. The result showed that 8 nucleotide sites and 4 haplotypes were found in the 458 bp long partial gene segment. The mean content of A+T (54.4%) was higher than G+C (45.7%). Genetic variation parameters showed the population of highest haplotype diversity was TL population (Hd=0.733), the population of highest nucleotide diversity was ZJ population (π=0.005). AMOVA showed that genetic variations among populations (74.5%) was higher than that within populations (25.5%). The tendency of molecular phylogenetic trees which were constructed by NJ and UPMGA method was similar. SC population and ZJ population were the closest genetically, and they clustered into a small branch, then with TL population. These findings were expected to provide reference information for the protection and utilization of germplasm resources of M.rosenbergii.

Key words: Macrobrachium rosenbergii, COⅠ gene, genetic diversity, genetic differentiation

中图分类号:

S917

蒋飞, 戴习林. 罗氏沼虾3个不同群体线粒体COⅠ基因序列变异及遗传多样性分析[J]. 渔业研究, 2023, 45(1): 8-13.

JIANG Fei, DAI Xilin. Analysis of the genetic diversity and sequence variation of mitochondrial COⅠgene from three populations of Macrobrachium rosenbergii[J]. Journal of Fisheries Research, 2023, 45(1): 8-13.

图/表 4

表1 3个罗氏沼虾群体遗传变异参数统计

Tab.1 Genetic variation parametric statistics of 3 populations of M.rosenbergii

群体 Populations	单倍型多样性指数 (Hd) Haplotype diversity	核苷酸多样性指数 (π) Nucleotide diversity	平均核苷酸差异数(K) Average number of nucleotide differences	Tajima's D检验统计量 Tajima's D test statistic	P值 P value
上海 SC	0	0	0	0	1.000
浙江ZJ	0.356	0.005	2.489	0.025	0.531
泰国TL	0.733	0.002	1.000	1.337	0.943
均值Mean	0.363	0.003	1.163	0.454	0.825

表2 3个罗氏沼虾群体间和群体内的分子方差分析(AMOVA)

Tab.2 Analysis of Molecular Variance among and within 3 populations of M.rosenbergii

变异来源 Source of variation	自由度 df	平方和 Sum of squares	遗传变异元素 Variance components	占总变异的百分比/% Percentage of variation
群体间Among population	2	35.133	1.699 Va	74.5
群体内Within population	27	15.700	0.5819 Vb	25.5
总计Total	29	50.833	2.280

表3 3个罗氏沼虾群体间遗传分化指数和群体遗传距离

Tab.3 Fixation index and genetic distance among 3 populations of M.rosenbergii

群体Populations	上海SC	浙江ZJ	泰国TL
上海SC	-	0.003	0.013
浙江ZJ	0.111	-	0.011
泰国TL	0.925	0.687	-

图 1 基于罗氏沼虾COⅠ基因构建的NJ系统树和UPMGA系统树注:SC、ZJ、TL分别是来自上海、浙江、泰国的罗氏沼虾;Mn为日本沼虾。

Fig.1 NJ and UPMGA phylogenetic tree based on COⅠgene sequences of M. rosenbergii Notes:SC,ZJ,TL were M.rosenbergii from Shanghai,Zhejiang,Thailand,respectively.Mn was M.nipponense.

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罗氏沼虾3个不同群体线粒体COⅠ基因序列变异及遗传多样性分析

Analysis of the genetic diversity and sequence variation of mitochondrial COⅠgene from three populations of Macrobrachium rosenbergii

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