富含ω-3多不饱和脂肪酸的磷脂酰丝氨酸制备工艺研究

doi:10.14012/j.cnki.fjsc.2022.06.008

渔业研究 ›› 2022, Vol. 44 ›› Issue (6): 599-605.DOI: 10.14012/j.cnki.fjsc.2022.06.008

富含ω-3多不饱和脂肪酸的磷脂酰丝氨酸制备工艺研究

廖妙飞,付万冬,孙继鹏,周宇芳,马明珠,王家星,姚玮,胡诗琦

浙江省海洋开发研究院，浙江舟山 316021

收稿日期:2022-08-01 修回日期:2022-10-18 出版日期:2022-12-25 发布日期:2023-01-05
通讯作者: 廖妙飞
作者简介:廖妙飞（1984-），女，硕士，高级工程师，从事海洋生物资源综合利用研究。E-mail：miaofeiliao@163.com
基金资助:
浙江省基础公益研究计划（LGN21C200001）；舟山市科技计划项目（2020C41007）

Study on the preparation of ω-3 polyunsaturated fatty acid phosphatidylserine

Received:2022-08-01 Revised:2022-10-18 Online:2022-12-25 Published:2023-01-05

摘要/Abstract

摘要： 本研究以南极磷虾油、L-丝氨酸为原料，在磷脂酶D（Phospholipase D-catalyzed，PLD）的催化作用下发生转酰基反应，制得富含二十碳五烯酸（Eicosapentaenoic acid，EPA）、二十二碳六烯酸（Decosahexaenoic acid，DHA）的磷脂酰丝氨酸（Phosphatidylserine，PS）。通过单因素和正交试验，确定了PS的最佳制备工艺条件：以乙酸乙酯为有机溶剂、水相pH 4.5、有机相与水相体积比1︰1、反应温度40℃、加酶量55U、L-丝氨酸与南极磷虾油质量比16︰1、反应时间8 h，在此条件下，PS得率为85.62%，产物中EPA含量为24.33%，DHA含量为19.95%。

关键词: 南极磷虾油, 磷脂酶D, 磷脂酰丝氨酸, ω-3多不饱和脂肪酸

Abstract: Phospholipase D-catalyzed transphosphatidylation from phosphatidylcholine of Antarctic krill oil to produceω-3 polyunsaturated fatty acid-riched phosphatidylserine in this study. Single factor and orthogonal experiments showed the optimal preparation conditions were: using ethyl acetate as organic solvent, aqueous phase pH 4.5, volume ratio of organic phase to aqueous phase 1︰1, reaction temperature 40℃, enzyme amount 55 U, mass ratio of L-serine to krill oil was 16︰1, reaction time 8 hours, the yield of phosphatidylserine was 85.62%, EPA content was 24.33%, DHA content was 19.95%.

Key words: Antarctic krill oil, Phospholipase D-catalyzed, phosphatidylserine, ω-3 polyunsaturated fatty acid

中图分类号:

S986.1

廖妙飞付万冬孙继鹏周宇芳马明珠王家星姚玮胡诗琦. 富含ω-3多不饱和脂肪酸的磷脂酰丝氨酸制备工艺研究[J]. 渔业研究, 2022, 44(6): 599-605.

参考文献

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富含ω-3多不饱和脂肪酸的磷脂酰丝氨酸制备工艺研究

Study on the preparation of ω-3 polyunsaturated fatty acid phosphatidylserine

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