Table of Content

24 April 2015, Volume 37 Issue 2

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Research on the biofloc bacterial community structure during larval rearing of Litopenaeus vannamei using metagenome sequencing

2015, 37(2):  91-97. 

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During larval rearing of Litopenaeus vannamei, the formation of biofloc in culture pond water could be facilitated after adding sucrose as the main carbohydrate sources. The analysis of biofloc taxonomic composition of metagenomic community showed that the species abundance and dominant bacteria in biofloc were fundamentally the same within experimental and control group in the level of phylum, class and genera, but the proportion of bacteria composition was different. The proportion of Alphaproteobacteria detected in experimental and control groups, which have the ability of removing contaminants, was 16.2% and 14.4%, respectively. The proportion of Pseudoalteromonas and Vibrio, which were the common pathogenic bacteria, was 1.3% and1.4% together with 0% and 0.1% in experimental and control groups. The paper provided the basic data for the application of biofloc technology in the process of shrimp nursery and breeding.

Isolation, identification and antibiotic sensitivity analysis of bacterial pathogen from skin ulcer disease in Apostichopus japonicus cultured in South China

2015, 37(2):  98-106. 

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One dominant bacterial strain was isolated from the skin of sick sea cucumber (Apostichopus japonicus) cultured in raft at Xiapu in Fujian. The strain FJXPH0612 was confirmed to be a bacterial pathogen of A. japonicus by artificial infection and was determined to be Vibrio harveyi based on morphological and physiological detection, biochemical tests, 16S rRNA gene analysis, and Biolog microbiol system identified. Antibiotic sensitivity analysis indicated that the pathogen was sensitive to azithromycin, spectinomycin, nitrofurantoin, chloramphenicol, cefepime, cefuroxime, cefotaxime, ceftazidime, cefoperazone and cotrimoxazole.

The influence of large scale aquaculture activities on tidal dynamics of Luoyuan Bay

2015, 37(2):  107-118. 

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Based on the measured data of tidal level and tidal flow in Luoyuan Bay from April to May in 2013, by comparison analysis of historical data and the numerical simulation results, tidal dynamic structure characteristics on large-scale high density aquaculture area and its response on aquaculture activities were studied in the paper. Tidal flow has two-layer structure from ebb-dominating near the surface to flood-dominating in the middle and bottom layers, and it flows in from the middle and bottom layers and flows out from the surface. The existence of high density aquaculture facilities and biology has hindered the gulf water flowing, resulting in horizontal flow speed having double boundary layer structure vertically, that is, the velocity in the surface and bottom layers is small while maximum value appears in the middle and lower part of the water, the average flow speed decreases overall, the nonlinear effect weakens, the degree of tidal wave deformation decreases.

The residues of dicofol in the surface waters, sediments and aquatic organisms from the Zhangjiang River Estuary of Fujian

2015, 37(2):  119-126. 

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The residues of dicofol (DCF) in the waters, sediments and aquatic organisms from Zhangjiang River Estuary were carried out by using GC-ECD. The residues levels, distribution patterns and possible sources of dicofol, as well as the risk to human health were also evaluated and discussed. The results showed that the concentrations of dicofol in surface layer waters ranged from ND (not detected) to 13.5ng?L-1, with a detection rate of 4.8%; concentrations of dicofol in surface sediments (dry weight) ranged from ND to 2.49 ng?g-1, with a detection rate of 28.6%; concentrations of dicofol in 11 species of aquatic organisms ranged from ND to 7.06 ng?g-1, with a detection rate of 38.1%. The distribution tendency of dicofol in surface waters and sediments from Zhangjiang Estuary were almost the same. The higher value of dicofol at D8 station both in the waters and sediments suggested that it might be related to the recent usage of dicofol to control mites by local farmers, and because of the usage of Dicofol caused new DDTs inputs at D8 station. Among 11 kinds of aquatic organisms, dicofol residues in hydrophyte were the lowest, while the residues in aquatic products ( fish, shrimp and shellfish) were relatively higher, but its residues level was still below the MRL (maximum residual limits) published by the European Community, USA and Japan. The risk of dicofol to human health was assessed according to ADI (acceptable daily intake) set by the national food safety standard. The MRL of dicofol based on ADI was much less than 1, which indicated that ERI (expose risk index) from the exposure to dicofol due to the consumption of aquatic products in Zhangjiang River Estuary waters was within acceptable ranges.

Community structure characteristics of the epibenthos in spring and autumn in Huangqi Bay of Fujian

2015, 37(2):  127-134. 

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In April of 2012, 2013 and 2014, and in November of 2012 and 2013, we had respectively made surveys of the Epibenthos in Lianjiang Huangqi Bay. 10 phyla (198 species) of the Epibenthos were identified and the most of them were annulate(121 species). There were only eight species appeared in the five surveys. In the five surveys, the range of biomass of the epibenthos was 13.503~27.092g/m2, and the spring was 18.142g/m2, the autumn 23.079g/m2. The range of abundance of the epibenthos was 208.3~2014.7ind/m2, and the spring was1046.0ind/m2, the autumn 292.5ind/m2. In biomass dominance, Annelida and Echinodermata occurred in spring and autumn alternatively. In abundance dominance, Annelida obviously predominated in spring and autumn. The dominant species changed greatly in spring and autumn during 2012 to 2014. In the five surveys, we only obtained the economic species in 2013, and the species was not much. The first dominant species is Annelid polychaetes. In April and November of 2012, April and November of 2013, April of 2014，the average of diversity index (H ') were 3.909、3.250、3.780、3.441 and 4.156, respectively, which indicated that the ecological environment of epibenthos in the survey place was not polluted or disturbed.

The effects of different K+ levels on the growth of Dunaliella salina

2015, 37(2):  135-139. 

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To explore appropriate concentration of K+ that was required to growth of Dunaliella salina,the effects of different K+ levels （1、4、8、12 mmol/L）on the growth of Dunaliella salina were studied under laboratory conditions. Using ANOVA and multiple comparisons, cell concentrations of Dunaliella salina on the 11th day were analyzed. The results showed that only between the group of 8 mmol/L and the group of 12 mmol/L had significant difference (0.01

Tracking survey after enhancement and releasing of Babylonia lutosa and its effects analysis

2015, 37(2):  140-147. 

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Combined with point-survey and productive survey by pot fisheries in 2012—2014，the stock enhancement effect of Babylonia lutosa in Huangqi Bay，Lianjiang county，Fujian province，was studied. The yield of Babylonia lutosa in point-survey by pot fisheries in June 2014 increased 25.1 times than those in 2012（before releasing）；by productive survey，the yield（107.84 kg），the catch per day（2.51 kg） and the catch one pot （120.50 g）of Babylonia lutosa in 2014 increased 2.8 times，2.7 times and 2.4 times than those in 2012（before releasing）；the mean weight of Babylonia lutosa caught in 2013 and 2014 reduced 73.98% and 67.45% than those in 2012（before releasing），and the formation of recruit population has been occurred in the releasing area，some small snails has been added to fishing population.By the two years stock enhancement，the recovery of Babylonia lutosa resource has had significant effect .

Studies on the morphological features and the growth performance of hybrid F1 of Epinephelus akaara (♀)×Epinephelus moara (♂)

2015, 37(2):  148-152. 

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To provide basic data for the economy utilization of interspecific hybridization of grouper species, we conducted hybridization between Epinephelus akaara (♀) and Epinephelus moara (♂) by artificial insemination. The morphological features and growth of the hybrid F1 were analyzed. The growth trend in hybrid F1 was close to the Epinephelus moara, the body morphological features and color in hybrid F1 were close to Epinephelus akaara. The hybrid F1 could live through winter in Fujian. These results suggested that the hybrids of Epinephelus akaara (♀)×E. moara (♂) had practical value in aquaculture.

Management of salmonella contamination risk of aquaculture products in raw processing

2015, 37(2):  153-156. 

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There are complicated factors which cause the aquaculture products to be contaminated by salmonella in raw processing. In this article we analyzed and evaluated the reasons of salmonella contamination in the raw processing of aquaculture products including the raw fish, the unhygienic contact surface, the unhealthy workers and the injurious insect. We also presented the risk management measures which could prevent the contamination and enhance the security of aquaculture products.

Design and implementation of an economic type water quality early warning machine

2015, 37(2):  157-161. 

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Aquaculture is that the profit and risk are relatively high industry. A large part of the risk is caused by poor water quality of aquaculture water. The remote online monitoring on the water quality data can enable farmers to master the real-time water quality not to limit the location. This paper designed and realized a kind of economical water quality early warning machine which could offer remote online monitoring platform for farmers to reduce the risk of disease and death of breeding object caused by adverse water quality.

The status and suggestions of Fujian fishery assistance in Xinjiang

2015, 37(2):  162-165. 

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Based on the existing situation of Fujian Fishery Aid to Xinjiang Project from 2011 to 2013, the main objective of this article was to present the current developing status of Fujian fishery assistance in Xinjiang, including the aspects of breeding varieties, technology, cooperation and exchange and so on. Furthermore, the some problems and deficiencies were analyzed and the concrete suggestions, such as taking fisheries aid as a media to promote the feelings between Fujian and Xinjiang, were proposed.

Advances in Studies of antioxidant active substances from seaweed of China

2015, 37(2):  166-171. 

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In recent years, a broad range of antioxidant active substances had been isolated and characterized from seaweed, including polysaccharides, polyphenols, superoxide dismutase, unsaturated fatty acids, taurine and vitamins, etc. Their origin, contents, structures, biological activities and corresponding mechanisms had been studied. Due to wide variety of supply and the advantages of potent antioxidant activities, complex structures and low side effects, the seaweed could be an ideal natural source for the development of marine drug and biological products. The research progress of antioxidant active substances in seaweed was systematically reviewed in this paper. These substances with the relevant researches were analyzed and discussed in an effort to provide a theoretical basis for the development of marine pharmaceutical and biological products.

Research advance on flesh quality improvement and evaluation of farmed fish

2015, 37(2):  172-178. 

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Currently, it is possible to modify flesh quality of farmed fish though genetic selection of superior strains and controlling diet compositions as well as culture environment. Flesh quality mainly was assessed by external appearance, biochemical compositions, sensory analysis and texture profile as well as muscle cellularity. The present review summarized the flesh quality evaluation methods and manipulation technologies of farmed fish so that some scientific references could be provided for future research in establishment of manipulation and evaluation standards of end product quality of farmed fish.