Schizochytrium limacinum SR21 is a kind of marine microalgae rich in DHA.The research group that the author joined previously found that SlMYB118 gene of S.limacinum SR21 was closely related to fatty acid synthesis and was a potential transcription factor for DHA synthesis regulation.In this paper,the full-length cDNA sequence of SlMYB118 gene was cloned by PCR and analyzed by bioinformatics method.The results predicted that SlMYB118 protein had an isoelectric point of 8.75,a molecular weight of 84.81 kDa,was localized in the nucleus,and had 106 potential phosphorylation sites.This study laid the theoretical foundation for the subsequent identification of the binding site of SlMYB118 gene,which was a key issue for the transcriptional regulatory mechanism and the establishment of the transcriptional regulatory network related to DHA synthesis.

By taking Thalassiosira weissflogii as the raw material,the effects of different concentrations of nitrogen and phosphorus nutrients on the growth and accumulation of fucoxanthin in algae were studied in this paper.Under different conditions,the cell density curve was drawn by sampling during the growth,the biomass was measured at the later growth stage,and the fucoxanthin was extracted with organic solvent and detected by the HPLC.The results showed that compared with other nitrogen nutrient treatments,urea group with 0.882 mmol·L^-1 concentration was the most suitable for the growth and fucoxanin accumulation of T.weissflogii,and there were significant differences(P<0.05).There was no significant difference in biomass accumulation of 0.054 mmol·L^-1 KH₂PO₄ group,but there was a significant difference in fucoxanthin content accumulation compared with other phosphorus nutrients(P<0.05).Under the condition of nitrogen or phosphorus deficiency,the growth and the accumulation of fucoxanthin in T.weissflogii were seriously affected.This study provided a theoretical basis for the optimization of culture medium and the high yield of fucoxanthin in T.weissflogii.