Abstract: In the present study，a bacterial strain was isolated from the hepatopancreas tissue of Litopenaeus vannamei in Fujian Province with suspected acute hepatopancreas necrosis disease. The 16S rRNA gene sequence，physiological and biochemical characteristics，mass - spectrometer characteristic analysis results showed that it was Vibrio parahemolyticus. Virulence gene detection showed that the strain could be positively amplified with the virulence genes including Pirvp? and PirvpB. However it showed negative amplification results for the thermostable direct hemolyticus gene (Tdh) and tdh - related hemolysin gene (Trh) . All of the health shrimps died at 72 hours after infected with the isolated strain，and infected shrimps presented with symptoms similar to the natural incidence. Drug resistance analysis showed that the isolate was sensitive to thiamphenicol， florfenicol and other 4 drugs. However it was resistant to ampicillin sodium，oxycycline，ciprofloxacin，doxycycline，and was intermediate to neomycin sulfate and enrofloxacin. The results provided basic data for epidemiological research and disease prevention of AHPND.