Abstract: Pyropia haitanensis, as an economically important cultivated seaweed in China . Conchospore releasing is a critical event of artificial cultivation of Pyropia, consequently the amount of conchospore released is very important to the success of the seedling collection. Based on an orthogonal design with 3 factors and 5 levels, the effects of temperature, salinity and light intensity on the conchospore releasing of P. haitanensis were studied. The results showed that temperature had a significant effect on the amount of conchospore released (F = 8.584, P = 0.002 < 0.05); salinity and light intensity had no significant effects on the amount of conchospores (F = 1.073, P = 0.412 > 0.05; F = 0.585, P = 0.680 > 0.05). Analysis of variance showed that temperature had a extremely significant effect on the amount of conchospore released from P. haitanensis (F = 9.215, P ≤ 0.01). Temperature was the first-order main effect, and the second-order interaction effect with salinity and light intensity was not significant (P?0.05). The optimal environmental conditions for the release of conchospore were as follows: temperature of 26 ℃, salinity of 1.018 ~ 1.023, and light intensity of 5000 ~ 8000Lux.