Abstract: Static adsorption and desorption experiments were analyzed to study on purification process of polyphenols from Sargassum fusiforme with macroporous adsorption resins. The results indicated that XDA-7 macroporous resin offered the highest adsorption and desorption capacity. The optimum purification conditions were as follows: concentration of sample was 3.20 mg/mL, velocity of sample solution was 1 BV/h, and 60% ethanol was taken as eluant with velocity of 2 BV/h. Under this condition, the purity of polyphenol product is 61.86 %.