5-羟色胺和氨海水对钝缀锦蛤解剖卵母细胞的体外促熟作用

doi:10.14012/j.cnki.fjsc.2022.05.006

渔业研究 ›› 2022, Vol. 44 ›› Issue (5): 477-483.DOI: 10.14012/j.cnki.fjsc.2022.05.006

5-羟色胺和氨海水对钝缀锦蛤解剖卵母细胞的体外促熟作用

巫旗生¹(), 祁剑飞¹, 宁岳¹, 罗娟¹, 庞小鹏², 郭香¹, 罗辉玉¹, 曾志南¹^,^*()

1.福建省水产研究所,福建省海洋生物增养殖与高值化利用重点实验室,福建省海洋生物资源开发利用协同创新中心,海洋生物种业技术国家地方联合工程研究中心,福建厦门 361013
2.集美大学水产学院,福建厦门 361021

收稿日期:2022-05-11 出版日期:2022-10-25 发布日期:2022-10-26
通讯作者: 曾志南(1963-),男,研究员,研究方向:海水贝类遗传育种.E-mail:xmzzn@sina.com
作者简介:巫旗生(1984-),男,助理研究员,硕士,研究方向:海水贝类遗传育种.E-mail:wqsljwjx@163.com
基金资助:
现代农业产业技术体系建设专项(CARS-49);国家海洋水产种质资源库项目;福建省省属公益类科研院所基本科研专项(2019R1013-12)

Effect of 5-HT and ammonia seawater on maturation of oocyte stripped from Tapes conspersus

WU Qisheng¹(), QI Jianfei¹, NING Yue¹, LUO Juan¹, PANG Xiaopeng², GUO Xiang¹, LUO Huiyu¹, ZENG Zhinan¹^,^*()

1. Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian Province,Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources,National and Local Joint Engineering Research Center of Marine Biological Seed Industry Technology,Fisheries Research Institute of Fujian,Xiamen 361013,China
2. Fisheries College,Jimei University,Xiamen 361021,China

Received:2022-05-11 Online:2022-10-25 Published:2022-10-26

摘要/Abstract

摘要：

采用解剖卵母细胞体外浸泡促熟的方法,研究了5-羟色胺(5-HT)和氨海水对钝缀锦蛤(Tapes conspersus)卵母细胞的促熟作用。结果表明,采用一定浓度的5-HT和氨海水浸泡均能够显著诱导钝缀锦蛤卵母细胞的生发泡破裂( P<0.05),5-HT和氨海水浸泡的卵母细胞受精率相差不大( P>0.05)。其中,10 μmol/L 5-HT浸泡卵母细胞60 min获得的受精率最高,达到48.57%;0.020%氨海水浸泡卵母细胞45 min获得的受精率最高,达到50.75%,但畸形较多。

关键词: 钝缀锦蛤, 卵母细胞, 体外促熟, 5-羟色胺, 氨海水

Abstract:

To construct the artificial breeding method of Tapes conspersus,effects of 5-hydroxytryptamine(5-HT)and ammonia seawater on the maturation of oocytes were studied by soaking the dissected oocytes in vitro.Results showed that both immersion with ammonia seawater and 5-hydroxytryptamine significantly increase the germinal vesicles breakdown in Tapes conspersus (P<0.05).However,the fertilization rate showed no significantly different between oocytes soaked in 5-hydroxytryptamine and ammonia seawater.The fertilization rate of oocytes treated with 10 μmol/L 5-HT for 60 min was 48.57%.The fertilization rate of oocytes treated with 0.020% ammonia seawater for 45 min was slightly higher(about 50.75%),whereas there were many malformations.

Key words: Tapes conspersus, oocytes, maturation improvement, 5-hydroxytryptamine, ammonia seawater

中图分类号:

S968.31

巫旗生, 祁剑飞, 宁岳, 罗娟, 庞小鹏, 郭香, 罗辉玉, 曾志南. 5-羟色胺和氨海水对钝缀锦蛤解剖卵母细胞的体外促熟作用[J]. 渔业研究, 2022, 44(5): 477-483.

WU Qisheng, QI Jianfei, NING Yue, LUO Juan, PANG Xiaopeng, GUO Xiang, LUO Huiyu, ZENG Zhinan. Effect of 5-HT and ammonia seawater on maturation of oocyte stripped from Tapes conspersus[J]. Journal of Fisheries Research, 2022, 44(5): 477-483.

图/表 6

图1 钝缀锦蛤解剖卵子的形态变化注:A.生发泡未破裂的卵细胞;B.生发泡破裂的卵细胞;C.正常发育的受精卵;D.畸形受精卵。

Fig.1 Morphology changes of oocyte stripped from T.conspersus Notes:A.Oocytes with germinal vesicle;B.GVBD of oocytes;C.The normal fertilized egg;D.Malformation.

图2 不同浓度 5-HT 对 GVBD 发生率的影响

Fig.2 Effect of different concentration of 5-HT on GVBD rate

图3 不同浓度 5-HT 对受精率的影响

Fig.3 Effect of different concentration of 5-HT on fertilizing rate

图4 不同浓度氨海水处理对 GVBD 发生率的影响

Fig.4 Effect of different concentration of AS on GVBD rate

图5 不同浓度氨海水处理对受精率的影响

Fig.5 Effect of different concentration of AS on fertilizing rate

表1 海水贝类卵母细胞经5-HT和氨海水浸泡获得的最高GVBD发生率和受精率

Tab.1 The highest incidence of GVBD and fertilization rate were obtained from seawater shellfish oocytes soaked in 5-HT and ammonia seawater

品种 Varieties	氨海水 Ammonia seawater		5-羟色胺 5-hydroxytryptamine(5-HT)		文献 References
品种 Varieties	GVBD发生率/% GVBD rate	受精率/% Fertilization rate	GVBD发生率/% GVBD tate	受精率/% Fertilization rate	文献 References
栉孔扇贝 Chlamys farrer	100	15.88	40	24.74	邸炜鹏等^[19]
马氏珠母贝 Pinctada fucata	-	-	59	-	王琦等^[22]
栉江珧 Atrina pectinata	90	88	33	5	李浩浩等^[27]
硬壳蛤 Mercenaria mercenaria	-	-	71.11	-	王清等^[32]
钝缀锦蛤 Tapes conspersus	84.44	50.75	50.86	48.57	本研究

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5-羟色胺和氨海水对钝缀锦蛤解剖卵母细胞的体外促熟作用

Effect of 5-HT and ammonia seawater on maturation of oocyte stripped from Tapes conspersus

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