欢迎访问《渔业研究》官方网站,今天是 分享到:

渔业研究 ›› 2022, Vol. 44 ›› Issue (5): 477-483.DOI: 10.14012/j.cnki.fjsc.2022.05.006

• 论文与报告 • 上一篇    下一篇

5-羟色胺和氨海水对钝缀锦蛤解剖卵母细胞的体外促熟作用

巫旗生1,祁剑飞1,宁岳1,罗娟1,庞小鹏2,郭香1,罗辉玉1,曾志南1*   


  1. 1.福建省水产研究所,福建省海洋生物增养殖与高值化利用重点实验室,福建省海洋生物资源开发利用协同创新中心,海洋生物种业技术国家地方联合工程研究中心,福建 厦门361013; 2.集美大学水产学院,福建 厦门 361021

  • 收稿日期:2022-05-11 修回日期:2022-05-18 出版日期:2022-10-25 发布日期:2022-10-26
  • 通讯作者: 曾志南
  • 作者简介:巫旗生(1984-),男,助理研究员,硕士,研究方向:海水贝类遗传育种.E-mail: wqsljwjx@163.com
  • 基金资助:
    现代农业产业技术体系建设专项(CARS-49);国家海洋水产种质资源库项目;福建省属公益类科研院所基本科研专项(2019R1013-12)

Effect of 5-HT and ammonia seawater on maturation of oocyte stripped from Tapes conspersus

  • Received:2022-05-11 Revised:2022-05-18 Online:2022-10-25 Published:2022-10-26

摘要: 采用解剖卵母细胞体外浸泡促熟的方法,研究了5-羟色胺(5-HT)和氨海水对钝缀锦蛤(Tapes conspersus)卵母细胞的促熟作用。结果表明,采用一定浓度的5-HT和氨海水浸泡均能够显著诱导钝缀锦蛤卵母细胞的生发泡破裂(P <0.05),5-HT和氨海水浸泡的卵母细胞受精率相差不大(P >0.05)。其中,10 μmol/L 5-HT浸泡卵母细胞60 min获得的受精率最高,达到48.57%;0.020%氨海水浸泡卵母细胞45 min获得的受精率最高,达到50.75%,但畸形较多。

关键词: 钝缀锦蛤, 卵母细胞, 体外促熟, 5-羟色胺, 氨海水

Abstract: To construct the artificial breeding method of Tapes conspersus, effects of 5-hydroxytryptamine (5-HT) and ammonia seawater on the maturation of oocytes were studied by soaking the dissected oocytes in vitro. Results showed that both immersion with ammonia seawater and 5-hydroxytryptamine significantly increase the germinal vesicles breakdown in Tapes conspersus (P < 0.05). However, the fertilization rate showed no significantly different between oocytes soaked in 5-hydroxytryptamine and ammonia seawater. The fertilization rate of oocytes treated with 10 μmol/L 5-HT for 60 min was 48.57%. The fertilization rate of oocytes treated with 0.020% ammonia seawater for 45 min was slightly higher (about 50.75%), whereas there were many malformations.

Key words: Tapes conspersus, oocytes, maturation improvement, 5-hydroxytryptamine, ammonia seawater

中图分类号: