关键词: 凡纳滨对虾, 选育, 仔虾幼体, 耐低温能力, Litopenaeus vannamei, breeding, larvae, low temperature resistance

Abstract: In order to explore the breeding techniques and methods of Litopenaeus vannamei, the comparative experiment on the low temperature resistance was conducted based on the larvae from four breeding populations of L. vannamei (A, B, C and D), including inbred progenies and hybrid progenies according to different mating combinations. In this experiment, the growth period of larvae was P11~P12; the body length was 6.09~7.54 mm; the water temperature was 13.5~19.0℃; and the experimental period was 48 h. According to the survival rates at the time of 24 h, 36 h and 48 h, the low temperature resistance of larvae from different provenances was evaluated comprehensively in all groups by combining the factor of body length. The results showed that there was a significant difference in the survival rate among all groups from the low temperature test of 24 h. At the time of 48 h during the experiment, the mating combination in which 4 groups had the highest survival rate and were related to seed shrimps of Population A (unrelated to those of Population D) had an average survival rate of 10.35% in these 16 mating combinations. An average survival rate of 0 was found in the mating combination in which 4 groups had the lowest survival rate and were not related to seed shrimps of Population A and also in the mating combination in which 3 groups were related to Population D. There was an extremely significant difference in the low temperature resistance of larvae from different provenances.