关键词: 高通量测序, 东海带鱼, 肠道, 菌群结构, high-throughput sequencing, Trichiurus haumela, intestinal flora, bacterial communities structure

Abstract: The structure of bacterial communities in intestinal flora of Trichiurus haumela was studied using high - throughput sequencing technology. As a result, a total of 36， 129 valid sequences were obtained,and clustering to 245 OUTs. Except for 4 sequences (0. 01%) could not be species classified，the rest belonged to 16 phyla，including 77 families， 127 genera. At the level of phylum， Proteobacteria was overwhelmingly dominant with high relative abundance up to 52.30%， followed by Firmicutes (30.66%) . At the level of genus， Photobacterium (47.90%) of Vibrionaceae (49.38%) and Candidatus 一 Arthromitus(17.04%) of Clostridiaceae一 1 (25.40%) were the main genera. Alpha diversity analysis showed that， the OTU dilution curve flatten out gradually，and the Good，s Coverage index was 100%， while the depth was large enough.