关键词: 孔石莼多糖, 锌, 络合, 响应面法, 工艺优化, Ulva pertusa polysaccharide, zinc, complexation, response surface method , optimization technology

Abstract: In the present study, the Box-Behnken design and the method of response surface (RSM) analysis with 3 factors and 3 levels was employed to optimize the complexation conditions between Ulva pertusa polysaccharide and Zn2+, and investigate the effects of temperature, pH and weight ratio between Ulva pertusa polysaccharide and Zn2+ on the contents of zinc in polysaccharide-Zn. The results showed that the optimum conditions of Ulva pertusa polysaccharide complexation Zn2+ required temperature as 50 ℃, pH as 5.0, polysaccharide-Zn2+ weight ratio as 2.8:1, the concentration of Zn2+ as 3.00 mg/ mL, and reaction time as 90 min. Under this condition, the contents of zinc in polysaccharide-Zn reached the maximum value as (102.41 ± 0.67) mg/g.