关键词: 不确定度, 丁香酚, 养殖水体, 气相色谱质谱, uncertainty, eugenol, aquaculture water, gas chromatography mass spectrumetric

Abstract: According to the procedure for uncertainty assessment，we evaluated the measurement uncertainty of detecting eugenol in aquaculture water by GC-MS method which established in our lab. The components of uncertainty in the detecting process were studied and analyzed, including standard work solution，weighing sample，constant sample solution volume，instrument detection and sample preparation repeatability, then calculating and combining these components of uncertainty. At the 95% confidence level，the coverage factor k= 2，expanding the method uncertainty. The results showed the major factors of uncertainty in this method were sample preparation repeatability and instrument detection. In order to insure the detecting results are accuracy and reliable, we should standardize the operation, strictly control environmental condition and increase parallel sample.