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›› 2016, Vol. 38 ›› Issue (4): 263-272.

• 论文与报告 •    下一篇

双斑东方鲀与红鳍东方鲀杂交育种研究初报

钟建兴,李雷斌,刘 波,方民杰,郑惠东   

  1. 福建省水产研究所
  • 收稿日期:2016-06-08 修回日期:2016-06-15 出版日期:2016-08-25 发布日期:2016-08-25
  • 通讯作者: 钟建兴
  • 基金资助:
    福建省海洋与渔业厅项目;厦门南方海洋研究中心项目;省属公益类科研院所基本科研专项;闽台重要海洋生物资源高值化开发技术公共服务平台;福建重要海洋经济生物种质库与资源高效开发技术公共服务平台

The primary research on crossbreeding between Fugu bimaculatus and Fugu rubripes

  • Received:2016-06-08 Revised:2016-06-15 Online:2016-08-25 Published:2016-08-25

摘要: 为获得具有杂交优势东方鲀苗种,开展了双斑东方鲀与红鳍东方鲀杂交组、对照组人工繁殖及育苗;正交组(SH)、反交组(HS)胚胎发育观察;杂交组、对照组幼鱼耐高温试验。结果表明:正交组、反交组、双斑东方鲀子一代对照组(SB)、红鳍东方鲀子一代对照组(HQ)卵受精率分别为95.6%、93.5%、96.0%、89.5%,受精卵孵化率分别为92.2%、80.6%、89.4%、79.9%,室内育苗成活率分别为28.4%、18.7%、27.5%、19.2%;正交组(SH)池塘生态培育成活率为18.3%;在18.0~20.0℃水温条件下,正交组(SH)受精卵经132 h孵出仔鱼,反交组(HS)受精卵经164 h40 min孵出仔鱼;在28.0~35.0℃水温条件,反交组(HS)、对照组(HQ)幼鱼不能存活,正交组(SH)、对照组(SB)幼鱼正常生长;杂交子代生物学特性与母本相近,正交组(SH)可在福建、广东、海南等南方沿海地区推广养殖。

关键词: 双斑东方鲀, 红鳍东方鲀, 杂交, 育种, Fugu bimaculatus, Fugu rubripes, hybirdization, breeding

Abstract: In order to obtain the juveniles of fugu with hybrid heterosis, the author applied artificial breeding technology in matured Fugu bimaculatus and Fugu rubripes for hybridization. The embryo development of normal crosses(SH) and reciprocal crosses (HS) were observed and the resistance test of high temperature in normal crosses (SH), reciprocal crosses (HS) and control groups (SB and HQ) were conducted. The results showed that the fertility rates were 95.6%, 93.5%, 96.0% and 89.5% in normal crosses(SH), reciprocal crosses (HS) and control groups (SB and HQ), respectively. The hatching rate of those oosperms were 92.2%、80.6%、89.4% and 79.9%, and the indoor survival rates of those juveniles were 28.4%、18.7%、27.5% and 19.2% in each group. The survival rate of juveniles of normal crosses(SH) in earth pond after ecological breeding was 18.3%. The oosperms of normal crosses(SH) with water temperature of 18.0~20.0℃ were developed into juveniles after 132 h. The juveniles of reciprocal crosses (HS) on the same conditions were developed into juveniles after 164 h40 min. The juveniles of reciprocal crosses(HS) and control groups(HQ) could not survive on the condition of water temperature from 28.0℃ to 35.0℃, on the other hand, the juveniles of normal crosses(SH) and control groups(SB) could grow normally on the same water temperature condition. The experiment indicated that the biological characteristics of hybrid descendants were close to female parent and the normal crosses (SH) might be broadly cultured in south coastal area such as Fujian, Guangdong, Hainan and so on.

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