关键词: Miseq高通量测序, 仿刺参, 池塘养殖, 肠道菌群, Miseq high-throughput sequencing, Apostichopus japonicus, pond culture, gut microbiota

Abstract: The gut microbiota is essential in shaping many of its host’s functional attributes. The gut microbiota is necessary for the proper physiological development of the gut and for the animal’s ability to digest and convert plant mass into food products. Thus based on high throughput sequencing technology MiSeq, the research aims at analyzing the structure of intestinal bacterial colonies of Apostichopus japonicus cultured in pond polyculture of Fujian. The results showed that the units of classification operation (OTUs) of the three samples were 424, 444, and 414, respectively. The dominant strains were quite different among these samples. The hindgut dominant bacteria were Haliea (11.97%) and Lactococcus (10.37%), while both foregut and midgut’s dominance were Lactococcus and Bacillus. In addition, the percentage of Lactococcus in foregut and midgut were 27.91% and 6.08%, respectively, as for Bacillus were 33.24% and 6.97%. In repeatability, colonies consisting of three samples had overlapping (74.35%), which foregut and midgut high similarity, the strain types more than 90% overlap. Therefore, the study aimed at providing basic information for the development and utilization of Apostichopus japonicu intestinal flora.