关键词: 河豚鱼, 织纹螺, 胶体金层析, 河豚毒素筛查, puffer fishes, nassarius, collaurum chromatography, tetrodotoxin screening

Abstract: Many people died for eating nocuous nassarius or puffer fishes which contain neurotoxic tetrodotoxin in southern China.The paper analyzed the application of the collaurum chromatography rapid detection card in nassarius and puffer fishes, and confirmed detection limit of kits was about 0.5MU/mL or 100ng/mL in tetrodotoxin aqueous solution. On the basis of limit value of tetrodotoxin, the rapid detection kits were designed for screening whether toxic content was above limit standard（10MU/g） in 20 samples including nassarius and puffer fishes, and precision rate reached 100%, single sample testing time was about 20 minutes. The kits have many advantages such as high sensitivity, good specificity, user-friendly control, rapid identification, non- apparatus supplementary, unaided eye results reading, and etc. The testing kits can be applied to testing organization at grassroots level, fishery management grassroots units, and aquatic product processor for screening tetrodotoxin in fishes and snails.