IAA浓度对细基江蓠繁枝变种切段再生芽的诱导效果

福建水产 ›› 2012, Vol. 34 ›› Issue (6): 481-487.

IAA浓度对细基江蓠繁枝变种切段再生芽的诱导效果

钟晨辉¹,黄瑞芳¹,郑雅友¹,李雷斌¹,刘波¹,林琪²

1. 福建省水产研究所
2.

收稿日期:2012-10-10 修回日期:2012-11-02 出版日期:2012-12-25 发布日期:2012-12-20
通讯作者: 钟晨辉
基金资助:
海洋公益性行业科研专项;福建省属公益类科研院所基本科研专项;福建省水产研究所青年科学创新基金

Effects of different concentrations of IAA on induction of asexual lines in Gracilaria tenuistipitata var. liui Zhang et Xia

Received:2012-10-10 Revised:2012-11-02 Online:2012-12-25 Published:2012-12-20
Contact: zhong chenhui

摘要/Abstract

摘要： 研究了在MES液体培养基内添加0.1～10.0 mg.L-1 IAA诱导细基江蓠繁枝变型藻体切段再生新芽形成无性系的效果。结果显示，藻体切段只能在形态学上端产生再生芽是细基江蓠繁枝变种的特性。与对照组相比，培养液添加0.1～5.0 mg.L-1低浓度IAA均能不同程度提高藻体切段切口处再生芽发芽率和生长率，其中添加2.0 mg.L-1 IAA对藻段再生芽生长的促进效果最佳，培养至35 d时，再生芽平均长度达到（25.99±1.28）mm，明显高于对照组平均长度（18.50±0.92）mm。然而，培养液添加10.0 mg.L-1高浓度的IAA，反而抑制了藻段再生芽的形成与生长，相比于对照组，再生芽数量最少，发芽率和生长率最低，培养至35 d时，再生芽平均长度仅为（12.23±0.85）mm,低于对照组。研究结果表明，IAA诱导江蓠再生芽的最佳浓度为2.0 mg.L-1，可为进一步构建细基江蓠繁枝变型种苗快繁应用技术提供基础。

关键词: IAA, 诱导, 再生芽, 细基江蓠繁枝变种, IAA, induction, asexual line, Gracilaria tenuistipitata var. liui Zhang et Xia

Abstract: Effects of MES liquid medium with the concentrations of 0.1～10.0 mg.L-1 IAA on induction of regenerate buds from algal fragments in Gracilaria tenuistipitata var. liui Zhang et Xia were studied. The results showed that the morphological upper ends of algal fragments characteristically germinated newborn buds that eventually developed into asexual lines. Compared to the control group，lower concentrations of 0.1～5.0 mg.L-1 IAA added in liquid culture medium all improved the sprouting rates and growth rates of regenerated buds in various degrees，and the promotion effect of 2.0 mg.L-1 IAA was the best，and the mean length of regenerated buds was (25.99±1.28)cm,obviously higher than that of the control with (18.50±0.92)cm after 35 days of culture. However，the group with concentration more than 10.0 mg.L-1 IAA restrained forming,and the mean length of regenerated buds was only (12.23±0.85)cm ,lower than that of the control group after being cultured for 35 days. It was indicated that the optimum concentration of IAA for inducing regenerated buds of the species was 2.0 mg.L-1. These base conclusions would be benefit for establishing further applied technique of rapid propagation in G. tenuistipitata var. liu.

钟晨辉黄瑞芳郑雅友李雷斌刘波林琪. IAA浓度对细基江蓠繁枝变种切段再生芽的诱导效果[J]. 福建水产, 2012, 34(6): 481-487.