水产品中麻痹性贝类毒素研究进展

doi:10.14012/j.cnki.fjsc.2024.02.012

摘要/Abstract

摘要：

近年来,有害藻华在中国沿海水域频繁发生。在有害藻华产生的藻毒素中,麻痹性贝类毒素(Paralytic shellfish toxins,PST)分布最广、危害最大,因而受到广泛关注。在被海洋生物摄食后,PST经食物链逐级向上传递、累积,这不仅影响生态环境安全,也威胁人类生命健康。因此,本文重点介绍了PST的定义、来源分布、危害、毒性机制、富集转化和检测方法(生物法、化学法和生物化学法),以及阐述PST的研究趋势、监管建议,旨在为完善PST研究资料、检测及防控措施提供参考。

关键词: 麻痹性贝类毒素, 毒性机制, 富集转化, 检测方法

Abstract:

In recent years, harmful algal blooms have frequently occurred in coastal waters of China. Among the microcystins produced by these blooms, paralytic shellfish toxins (PST) are the most widely distributed and harmful, leading to significant concerns. When marine organisms ingest PST, they are passed up the food chain and accumulate, eventually posing a serious threat to human health, the environment, and the ecosystem.Therefore, this paper primarily introduces the definition, distribution, harm, toxicity mechanism, enrichment, and transformation of PST. It also discusses the methods used to detect PST, including biological, chemical, and biochemical approaches. The paper expounds on research trends and regulatory recommendations for PST. Its aim is to serve as a reference for the improvement of research, detection, and prevention of PST.

Key words: paralytic shellfish toxins, toxic mechanism, enrichment and transformation, detection method

中图分类号:

Q586

牛耀路, 苏捷. 水产品中麻痹性贝类毒素研究进展[J]. 渔业研究, 2024, 46(2): 207-214.

NIU Yaolu, SU Jie. Research progress of paralytic shellfish toxins in aquatic products[J]. Journal of Fisheries Research, 2024, 46(2): 207-214.

参考文献 71

[1]	Wang D, Zhang S, Zhang H, et al. Omics study of harmful algal blooms in China: current status, challenges, and future perspectives[J]. Harmful Algae, 2021, 107: 102079. DOI URL
[2]	王思远, 张保军, 王昊, 等. 基于三维荧光的产麻痹性贝毒藻浓度监测研究[J]. 光谱学与光谱分析, 2021, 41(11): 3480-3485.
[3]	Hess P. Poisoning caused by marine biotoxins[J]. Bundesgesundheitsblatt-Gesundheitsforschung-Gesundheitsschutz, 2017, 60(7): 757-760. DOI URL
[4]	包振民, 孔令玲, 史姣霞, 等. 双壳贝类积累转化麻痹性贝毒的研究进展[J]. 中国海洋大学学报(自然科学版), 2021, 51(10): 1-11.
[5]	杜克梅, 江天久, 吴霓. 黄海海域贝类麻痹性贝类毒素污染状况研究[J]. 海洋环境科学, 2013, 32(2): 182-184.
[6]	闫鹏, 郑代丰, 章群. 麻痹性贝类毒素分析技术研究进展[J]. 中国卫生检验杂志, 2021, 31(12): 1532-1536.
[7]	孙烨. 麻痹性贝类毒素细胞检测法的建立与评价[D]. 长沙: 湖南师范大学, 2016.
[8]	Wiese M, D’Agostino P M, Mihali T K, et al. Neurotoxic alkaloids: saxitoxin and its analogs[J]. Marine Drugs, 2010, 8(7): 2185-2211. DOI PMID
[9]	田娟娟, 韩刚, 刘海棠, 等. 国内外麻痹性贝类毒素风险预警及管控措施的比对分析[J]. 海洋环境科学, 2019, 38(3): 464-470.
[10]	Etheridge S M. Paralytic shellfish poisoning: seafood safety and human health perspectives[J]. Toxicon, 2010, 56(2): 108-122. DOI PMID
[11]	汪宇. 贻贝中麻痹性贝类毒素的来源解析及其形成机制初步研究[D]. 上海: 上海海洋大学, 2021.
[12]	中华人民共和国国家卫生和计划生育委员会. 食品安全国家标准鲜、冻动物性水产品: GB 2733—2015[S]. 北京: 中国标准出版社, 2015.
[13]	刘智勇, 计融. 各国贝类水产品中麻痹性贝类毒素限量标准的比对[J]. 中国热带医学, 2006, 6(1): 176-178.
[14]	宋维佳, 宋秀贤, 俞志明, 等. 海洋中麻痹性贝类毒素的合成转化及其影响因素研究进展[J]. 海洋科学, 2022, 46(9): 117-129.
[15]	刘仁沿, 马德毅, 梁玉波. 赤潮甲藻毒素麻痹性贝毒的生物合成研究进展[J]. 海洋环境科学, 2004, 23 (4): 71-75.
[16]	Peacock M B, Gibble C M, Senn D B, et al. Blurred lines: multiple freshwater and marine algal toxins at the land-sea interface of San Francisco Bay, California[J]. Harmful Algae, 2018, 73: 138-147. DOI PMID
[17]	Anderson D M, Cembella A D, Hallegraeff G M. Progress in understanding harmful algal blooms: paradigm shifts and new technologies for research, monitoring, and management[J]. Annual Review of Marine Science, 2012, 4: 143-176. PMID
[18]	Shin C, Jo H, Kim S H, et al. Exposure assessment to paralytic shellfish toxins through the shellfish consumption in Korea[J]. Food Research International, 2018, 108: 274-279. DOI PMID
[19]	翟毓秀, 郭萌萌, 江艳华, 等. 贝类产品质量安全风险分析[J]. 中国渔业质量与标准, 2020, 10(4): 1-25.
[20]	于仁成, 吕颂辉, 齐雨藻, 等. 中国近海有害藻华研究现状与展望[J]. 海洋与湖沼, 2020, 51(4): 768-788.
[21]	Fernández B, Campillo J A, Martínez-Gómez C, et al. Assessment of the mechanisms of detoxification of chemical compounds and antioxidant enzymes in the digestive gland of mussels, Mytilus galloprovincialis, from Mediterranean coastal sites[J]. Chemosphere, 2012, 87(11): 1235-1245. DOI PMID
[22]	Fernández B, Campillo J A, Martínez-Gómez C, et al. Antioxidant responses in gills of mussel (Mytilus galloprovincialis) as biomarkers of environmental stress along the Spanish Mediterranean coast[J]. Aquatic Toxicology, 2010, 99(2): 186-197. DOI PMID
[23]	Catterall W A. The molecular basis of neuronal excitability[J]. Science, 1984, 223(4637): 653-661. PMID
[24]	Catterall W A. From ionic currents to molecular mechanisms: the structure and function of voltage-gated sodium channels[J]. Neuron, 2000, 26(1): 13-25. DOI PMID
[25]	Catterall W A, Cestèle S, Yarov-Yarovoy V, et al. Voltage-gated ion channels and gating modifier toxins[J]. Toxicon, 2007, 49(2): 124-141. DOI PMID
[26]	Kang H M, Lee J, Lee Y J, et al. Transcriptional and toxic responses to saxitoxin exposure in the marine copepod Tigriopus japonicus[J]. Chemosphere, 2022, 309: 136464. DOI URL
[27]	Akbar M A, Mohd Yusof N Y, Tahir, N I, et al. Biosynthesis of saxitoxin in marine dinoflagellates: an omics perspective[J]. Marine Drugs, 2020, 18(2): 103. DOI URL
[28]	Stevens M, Peigneur S, Tytgat J. Neurotoxins and their binding areas on voltage-gated sodium channels[J]. Frontiers in Pharmacology, 2011, 2:71. DOI PMID
[29]	O’Neill K, Musgrave I F, Humpage A. Low dose extended exposure to saxitoxin and its potential neurodevelopmental effects: a review[J]. Environmental Toxicology and Pharmacology, 2016, 48: 7-16. DOI PMID
[30]	Noda M, Suzuki H, Numa S, et al. A single point mutation confers tetrodotoxin and saxitoxin insensitivity on the sodium channel Ⅱ[J]. FEBS Letters, 1989, 259(1): 213-216. PMID
[31]	Terlau H, Heinemann S H, Stühmer W, et al. Mapping the site of block by tetrodotoxin and saxitoxin of sodium channel Ⅱ[J]. FEBS Letters, 1991, 293(1-2): 93-96. PMID
[32]	Llewellyn L E. Saxitoxin, a toxic marine natural product that targets a multitude of receptors[J]. Natural Product Reports, 2006, 23(2): 200-222. PMID
[33]	Shumway S E. A review of the effects of algal blooms on shellfish and aquaculture[J]. Journal of the World Aquaculture Society, 1990, 21(2): 65-104. DOI URL
[34]	呼晓群, 解万翠, 李敏, 等. 贝类中麻痹性贝类毒素的蓄积及代谢研究进展[J]. 食品与机械, 2021, 37(3): 187-194.
[35]	江天久, 尹伊伟, 骆育敏, 等. 大亚湾和大鹏湾麻痹性贝类毒素动态分析[J]. 海洋环境科学, 2000, 19(2): 1-5.
[36]	Terrazas J O, Contreras H R, García C. Prevalence, variability and bioconcentration of saxitoxin-group in different marine species present in the food chain[J]. Toxins, 2017, 9(6): 190. DOI URL
[37]	Navarro J M, González K, Cisternas B, et al. Contrasting physiological responses of two populations of the razor clam Tagelus dombeii with different histories of exposure to paralytic shellfish poisoning (PSP)[J]. PLoS One, 2014, 9(8): e105794. DOI URL
[38]	Li A, Ma J, Cao J, et al. Analysis of paralytic shellfish toxins and their metabolites in shellfish from the North Yellow Sea of China[J]. Food Additives & Contaminants: Part A, 2012, 29(9): 1455-1464.
[39]	García C, Barriga A, Díaz J C, et al. Route of metabolization and detoxication of paralytic shellfish toxins in humans[J]. Toxicon, 2010, 55(1): 135-144. DOI PMID
[40]	邱江兵. 双壳贝类对麻痹性贝毒的代谢转化及其生理生化响应[D]. 青岛: 中国海洋大学, 2014.
[41]	Blanco J, Reyero M I, Franco J. Kinetics of accumulation and transformation of paralytic shellfish toxins in the blue mussel Mytilus galloprovincialis[J]. Toxicon, 2003, 42(7): 777-784. PMID
[42]	Kotaki Y. Screening of bacteria which convert gonyautoxin 2, 3 to saxitoxin[J]. Nippon Suisan Gakkaishi, 1989, 55(7): 1293-1293. DOI URL
[43]	Kotaki Y, Oshima Y, Yasumoto T. Bacterial transformation of paralytic shellfish toxins in coral-reef crabs and a marine snail[J]. Nippon Suisan Gakkaishi, 1985, 51(6): 1009-1013. DOI URL
[44]	Sullivan J. Paralytic shellfish poisoning: analytical and biochemical investigations[J]. ИНФОРМАЦИЯ О ПУБЛИКАЦИИ, 1983, 1:7381772.
[45]	Shimizu Y, Yoshioka M. Transformation of paralytic shellfish toxins as demonstrated in scallop homogenates[J]. Science, 1981, 212(4494): 547-549. PMID
[46]	Jones G J, Bourne D G, Blakeley R L, et al. Degradation of the cyanobacterial hepatotoxin microcystin by aquatic bacteria[J]. Natural Toxins, 1994, 2(4): 228-235. PMID
[47]	Smith E A, Grant F, Ferguson C M, et al. Biotransformations of paralytic shellfish toxins by bacteria isolated from bivalve molluscs[J]. Applied and Environmental Microbiology, 2001, 67(5): 2345-2353. PMID
[48]	Lee N S, Kim B T, Kim D H, et al. Purification and reaction mechanism of arylsulfate sulfotransferase from Haemophilus K-12, a mouse intestinal bacterium[J]. The Journal of Biochemistry, 1995, 118(4): 796-801. DOI URL
[49]	周磊, 杨宪立, 武爱波, 等. 麻痹性贝类毒素的安全评价与检测技术研究进展[J]. 世界科技研究与发展, 2014, 36(3):336-342.
[50]	Tan K, Ransangan J. Factors influencing the toxicity, detoxification and biotransformation of paralytic shellfish toxins[J]. Reviews of Environmental Contamination and Toxicology, 2015, 235: 1-25. DOI PMID
[51]	黄宗锈, 林健, 陈冠敏, 等. 麻痹性贝类毒素小鼠生物法检测中位数法与均数法差异性比较[J]. 中国卫生检验杂志, 2008, 18(2): 369-370.
[52]	朱敬萍, 金雷, 张小军, 等. 小鼠生物法检测麻痹性贝类毒素技术探讨及应用[J]. 中国渔业质量与标准, 2017, 7(2): 30-35.
[53]	丁君. 赤潮毒素中腹泻性贝毒和麻痹性贝毒的研究及进展[J]. 大连水产学院学报, 2001, 16(3): 212-218.
[54]	黄奕雯. 石房蛤毒素胶体金快速检测试纸卡的应用研究[J]. 渔业研究, 2020, 42(6): 614-621.
[55]	黄爱君, 黄海燕, 刘建军. 麻痹性和腹泻性贝类毒素的检测方法研究进展[J]. 环境与健康杂志, 2010, 27(1): 84-86.
[56]	杜伟, 王扬, 张晓辉, 等. 小鼠生物法与ELISA法检测麻痹性贝类毒素的比较[J]. 浙江农业科学, 2015, 56(11): 1726-1728.
[57]	汤云瑜, 黄冬梅, 蔡友琼. 麻痹性贝类毒素检测技术研究[J]. 农产品质量与安全, 2020(6): 29-34.
[58]	张杭君, 张建英. 麻痹性贝毒素的毒理效应及检测技术[J]. 海洋环境科学, 2003, 22(4): 76-80.
[59]	杨云辉. HPLC-MS/MS法检测福建中部海域养殖贝类麻痹性贝类毒素[J]. 渔业研究, 2020, 42(5): 473-480.
[60]	Deng Y, Zheng H, Yi X, et al. Paralytic shellfish poisoning toxin detection based on cell-based sensor and non-linear signal processing model[J]. International Journal of Food Properties, 2019, 22(1): 890-897. DOI URL
[61]	Ferreira N S, Cruz M G N, Gomes M T S R, et al. Potentiometric chemical sensors for the detection of paralytic shellfish toxins[J]. Talanta, 2018, 181: 380-384. DOI PMID
[62]	Li H, Wei X, Gu C, et al. A dual functional cardiomyocyte-based hybrid-biosensor for the detection of diarrhetic shellfish poisoning and paralytic shellfish poisoning toxins[J]. Analytical Sciences, 2018, 34(8): 893-900. DOI PMID
[63]	Campàs M, Marty J L. Enzyme sensor for the electrochemical detection of the marine toxin okadaic acid[J]. Analytica Chimica Acta, 2007, 605(1): 87-93. PMID
[64]	Campàs M, Prieto-Simón B, Marty J L. Biosensors to detect marine toxins: assessing seafood safety[J]. Talanta, 2007, 72(3): 884-895. DOI PMID
[65]	张庆芳, 韩鹏飞, 谢丹丹, 等. 海洋降解麻痹性贝类毒素细菌的筛选及其耐毒能力初探[J]. 饲料博览, 2021(10): 5-10.
[66]	Reboreda A, Lago J, Chapela M J, et al. Decrease of marine toxin content in bivalves by industrial processes[J]. Toxicon, 2010, 55(2-3): 235-243. DOI PMID
[67]	Xie W C, Liu X L, Yang X H, et al. Accumulation and depuration of paralytic shellfish poisoning toxins in the oyster Ostrea rivularis Gould-chitosan facilitates the toxin depuration[J]. Journal of Food Control, 2013, 30(2): 446-452. DOI URL
[68]	Li J, Song X X, Zhang Y, et al. Effect of modified clay on the transition of paralytic shellfish toxins within the bay scallop Argopecten irradians and sediments in laboratory trials[J]. Aquaculture, 2019, 505: 112-117. DOI URL
[69]	Qiu J B, Fan H, Liu T, et al. Application of activated carbon to accelerate detoxification of paralytic shellfish toxins from mussels Mytilus galloprovincialis and scallops Chlamys farreri[J]. Ecotoxicology and Environmental Safety, 2018, 148: 402-409. DOI URL
[70]	吴益春, 郭海波, 罗海军, 等. 海产品中麻痹性贝类毒素快速检测技术研究进展[J]. 食品安全质量检测学报, 2019, 10(8): 2092-2096.
[71]	于仁成, 罗璇. 我国近海有毒藻和藻毒素的研究现状与展望[J]. 海洋科学集刊, 2016(1): 155-166.