黑斑原鮡对急性高温耐受性的研究

doi:10.14012/j.cnki.fjsc.2024.02.006

渔业研究 ›› 2024, Vol. 46 ›› Issue (2): 156-161.DOI: 10.14012/j.cnki.fjsc.2024.02.006

黑斑原鮡对急性高温耐受性的研究

曾荣俊¹(), 刘晓锐¹, 耿庆宝¹, 唐雪梅², 方媛林², 吴雷³, 张欢³, 顾正选³, 禹猛³

1.华能西藏雅鲁藏布江水电开发投资有限公司藏木水电厂,西藏山南 856400
2.四川律贝生物科技有限公司,四川成都 610000
3.武汉中科瑞华生态科技股份有限公司,湖北武汉 430077

收稿日期:2023-10-11 出版日期:2024-04-25 发布日期:2024-04-25
作者简介:曾荣俊(1982—),男,高级工程师,学士,研究方向:水电厂生态环境保护。E-mail:13928167@qq.com
基金资助:
华能西藏雅鲁藏布江水电开发投资有限公司科研项目(JC2020/P07)

Effects of high temperature stress on the lethality of fry and adult Glyptosternon maculatum

ZENG Rongjun¹(), LIU Xiaorui¹, GENG Qingbao¹, TANG Xuemei², FANG Yuanlin², WU Lei³, ZHANG Huan³, GU Zhengxuan³, YU Meng³

1. Zangmu Hydropower Plant, Huaneng Tibet Yarlung Zangbo Hydropower Development Investment Co., Ltd., Shannan 856400, China
2. Sichuan Aquabase Biotechnology Co., Ltd., Chengdu 610000, China
3. Wuhan Zhongke Ruihua Ecological Technology Co., Ltd., Wuhan 430077, China

Received:2023-10-11 Online:2024-04-25 Published:2024-04-25

摘要/Abstract

摘要：

为探究黑斑原鮡(Glyptosternon maculatum)鱼苗与成鱼对急性高温胁迫的耐受情况,本研究测定了鱼苗与成鱼在不同急性高温胁迫下的半致死时间,以及成鱼的呼吸频率。结果表明:黑斑原鮡鱼苗的半致死时间(LT₅₀)随水温的升高而缩短,不同水温组的半数死亡时间存在显著差异(P<0.05),其中28 ℃组在13.53 h可达到半数死亡,24 ℃则为71.20 h;在高温胁迫下,成鱼的呼吸频率显著升高,24 ℃组的半致死时间(LT₅₀)为15.60 h,远短于鱼苗在该水温下的半致死时间。急性高温胁迫下黑斑原鮡鱼苗的耐受能力强于成鱼,但两者均容易在短期内发生大量死亡,因此在养殖过程中应时刻关注水温的变化。

关键词: 黑斑原鮡, 鱼苗, 成鱼, 急性高温胁迫, 半致死时间

Abstract:

In order to explore the tolerance of Glyptosternon maculatum to extreme temperatures at different stages, this study performed acute hyperthermic stress on the fry and adult fish, determined semi-lethal time at each temperature, and measured the respiratory rate of adult fish under hyperthermia. The results showed that the semi-lethal time (LT₅₀) of fry G.maculatum shortened with the increase of temperature, and there was a significant difference in the LT₅₀ of the different temperature groups (P<0.05), among which LT₅₀ was 13.53 h at 28 ℃ and 71.20 h at 24 ℃. The respiratory rate of adult fish under high temperature stress increased significantly, and the semi-lethal time (LT₅₀) of adult fish at 24 ℃ was 15.6 h, which was much lower than that of fry at this temperature. The results showed that there were differences in heat tolerance at different growth stages, and attention should be paid to water temperature control during the breeding process. The short-term lethal temperature of fry was 28 ℃, and the short-term lethal temperature of adult fish was 24 ℃. The results of this study can provide basic data for healthy breeding and further study of high temperature tolerance mechanism of G.maculatum.

Key words: Glyptosternon maculatum, fry, adult fish, acute hyperthermia stress, semi-lethal time

中图分类号:

S962

曾荣俊, 刘晓锐, 耿庆宝, 唐雪梅, 方媛林, 吴雷, 张欢, 顾正选, 禹猛. 黑斑原鮡对急性高温耐受性的研究[J]. 渔业研究, 2024, 46(2): 156-161.

ZENG Rongjun, LIU Xiaorui, GENG Qingbao, TANG Xuemei, FANG Yuanlin, WU Lei, ZHANG Huan, GU Zhengxuan, YU Meng. Effects of high temperature stress on the lethality of fry and adult Glyptosternon maculatum[J]. Journal of Fisheries Research, 2024, 46(2): 156-161.

图/表 3

参考文献 23

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温度/℃ Temperature	致死时间/min Lethal time
温度/℃ Temperature	10%	20%	30%	40%	50%(LT₅₀)
22	32.60±4.40^a	46.53±3.06^a	59.93±1.91^a	66.80±0.00^a	—
24	33.53±7.22^a	45.67±4.04^a	49.40±1.98^b	64.90±4.80^a	71.20±0.00^a
26	24.67±7.03^a	32.77±1.27^b	39.17±5.48^c	45.33±6.75^b	52.86±4.21^b
28	8.50±0.87^b	9.93±1.01^c	11.33±1.72^d	13.20±0.00^c	13.53±0.28^c

温度/℃ Temperature	致死时间/min Lethal time
温度/℃ Temperature	10%	20%	30%	40%	50%(LT₅₀)
22	32.60±4.40^a	46.53±3.06^a	59.93±1.91^a	66.80±0.00^a	—
24	33.53±7.22^a	45.67±4.04^a	49.40±1.98^b	64.90±4.80^a	71.20±0.00^a
26	24.67±7.03^a	32.77±1.27^b	39.17±5.48^c	45.33±6.75^b	52.86±4.21^b
28	8.50±0.87^b	9.93±1.01^c	11.33±1.72^d	13.20±0.00^c	13.53±0.28^c

组别 Groups	温度/℃ Temperature	溶解氧/(mg/L) Dissolved oxygen	呼吸频率/(次/min) Respiratory frequency
对照组Control group	12	6.52±0.17	89.00±4.58
试验组Experiment group	24	4.68±0.16^*	205.33±6.43^*

组别 Groups	温度/℃ Temperature	溶解氧/(mg/L) Dissolved oxygen	呼吸频率/(次/min) Respiratory frequency
对照组Control group	12	6.52±0.17	89.00±4.58
试验组Experiment group	24	4.68±0.16^*	205.33±6.43^*

时间/h Time	累计死亡数/ind Cumulative number of deaths	累计死亡率/% Cumulative death rate
4.10	5	16.67
4.60	6	20.00
6.60	8	26.67
9.40	9	30.00
9.60	10	33.33
11.10	11	36.67
12.00	13	43.33
14.40	14	46.67
15.60	15	50.00

黑斑原鮡对急性高温耐受性的研究

Effects of high temperature stress on the lethality of fry and adult Glyptosternon maculatum

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