环境DNA宏条形码在浮游动植物多样性研究中的应用

doi:10.14012/j.cnki.fjsc.2023.04.010

渔业研究 ›› 2023, Vol. 45 ›› Issue (4): 399-407.DOI: 10.14012/j.cnki.fjsc.2023.04.010

环境DNA宏条形码在浮游动植物多样性研究中的应用

吴泳江¹^,²(), 蔡明成¹^,², 孙翰昌¹^,²^,^*(), 胡慧蝶¹^,²^,³, 邓雅心¹^,²^,³, 龙应根¹^,²^,³

1.重庆文理学院园林与生命科学学院,重庆402160
2.重庆文理学院生态渔业产业化技术创新中心,重庆 402160
3.重庆三峡学院生物与食品工程学院,重庆 404100

收稿日期:2023-02-17 出版日期:2023-08-25 发布日期:2023-08-09
通讯作者: 孙翰昌(1978—),男,教授,研究方向为渔业资源评估和生态补偿策略、水产品质量安全检测与评价等。E-mail:sunhanchang199@163.com
作者简介:吴泳江(1990—),男,讲师,博士,主要从事动物营养与水产动物生态健康养殖研究。E-mail:wyongjang@163.com
基金资助:
重庆市技术创新与应用发展专项重点项目(cstc2019jscx-gksbX0147);重庆长江流域水生生物资源及重要生境监测调查项目(21C01467);重庆市水产科技创新联盟科技创新重点攻关项目(2022)

Application of environmental DNA metabarcoding in studying biodiversity of zooplankton and phytoplankton

WU Yongjiang¹^,²(), CAI Mingcheng¹^,², SUN Hanchang¹^,²^,^*(), HU Huidie¹^,²^,³, DENG Yaxin¹^,²^,³, LONG Yinggen¹^,²^,³

1. College of Landscape Architecture and Life Science, Chongqing University of Arts and Sciences, Chongqing 402160, China
2. Technology Innovation Center of Ecological Fisheries Industrialization, Chongqing University of Arts and Sciences,Chongqing 402160, China
3. College of Biology and Food Engineering, Chongqing Three Gorges University, Chongqing 404100, China

Received:2023-02-17 Online:2023-08-25 Published:2023-08-09

摘要/Abstract

摘要：

浮游植物和浮游动物分别是水生生态系统的初级生产者和消费者,浮游动植物的准确识别鉴定是开展水生生态学研究的基础。传统的基于物种形态特征鉴定浮游动植物费时费力,鉴定困难,也低估了物种多样性。因此,亟需一套简单、高效、准确、标准化的解决方案,用于浮游动植物多样性研究。环境DNA(Environmental DNA,eDNA)宏条形码技术已成为水生生物多样性研究的常用方法,主要被应用于鱼类物种多样性研究,近年来也逐渐被用于浮游动植物多样性研究中。本文首先介绍了eDNA宏条形码技术及其采样方法,再重点综述该技术在浮游动植物研究中的应用,以推动eDNA宏条形码技术在浮游生物多样性研究上的发展。

关键词: 环境DNA, 宏条形码, 浮游植物, 浮游动物, 生物多样性

Abstract:

Phytoplankton and zooplankton are the primary producers and consumers of aquatic ecosystems, respectively. Their accurate identification is the basis for aquatic ecology research. Traditional identification of phytoplankton and zooplankton based on morphological characteristics of species is time-consuming and labor-consuming, difficulty in identification, and underestimating species diversity. Therefore, a simple, efficient, accurate and standardized solution for studying biodiversity of zooplankton and phytoplankton is very necessary. Environmental DNA (eDNA) metabarcoding technique has become a common method for studying aquatic biodiversity. It is mainly used in the studying of fish species diversity and has also been gradually used in the studying biodiversity of zooplankton and phytoplankton in recent years. This review firstly introduced the eDNA metabarcoding technique and its sampling method, and then focused on the application of this technique in the studying of biodiversity of zooplankton and phytoplankton to promote the development of eDNA metabarcoding technique in plankton diversity research.

Key words: environmental DNA, metabarcoding, phytoplankton, zooplankton, biodiversity

中图分类号:

S932.2

吴泳江, 蔡明成, 孙翰昌, 胡慧蝶, 邓雅心, 龙应根. 环境DNA宏条形码在浮游动植物多样性研究中的应用[J]. 渔业研究, 2023, 45(4): 399-407.

WU Yongjiang, CAI Mingcheng, SUN Hanchang, HU Huidie, DENG Yaxin, LONG Yinggen. Application of environmental DNA metabarcoding in studying biodiversity of zooplankton and phytoplankton[J]. Journal of Fisheries Research, 2023, 45(4): 399-407.

图/表 2

参考文献 67

[1]	张宛宛, 谢玉为, 杨江华, 等. DNA宏条形码(metabarcoding)技术在浮游植物群落监测研究中的应用[J]. 生态毒理学报, 2017, 12(1): 15-24.
[2]	冯芸芝, 孙栋, 邵倩文, 等. DNA宏条形码技术在海洋浮游动物多样性和生态学研究中的应用[J]. 生态学报, 2022, 42(21): 1-11.
[3]	Shokralla S, Spall J L, Gibson J F, et al. Next-generation sequencing technologies for environmental DNA research[J]. Molecular Ecology, 2012, 21(8): 1794-1805. DOI PMID
[4]	Wang S P, Yan Z G, Hanfling B, et al. Methodology of fish eDNA and its applications in ecology and environment[J]. Science of the Total Environment, 2021, 755(2):142622. DOI URL
[5]	Harrison J B, Sunday J M, Rogers S M. Predicting the fate of eDNA in the environment and implications for studying biodiversity[J]. Proceedings of the Royal Society B-Biological Sciences, 2019, 286(1915): 1409-1415.
[6]	杨子萍, 李大命, 刘燕山, 等. 基于Cyt b序列的太湖和洪泽湖翘嘴鲌遗传多样性和遗传结构分析[J]. 渔业研究, 2023, 45(1): 1-7. DOI
[7]	蒋飞, 戴习林. 罗氏沼虾3个不同群体线粒体COⅠ基因序列变异及遗传多样性分析[J]. 渔业研究, 2023, 45(1): 8-13. DOI
[8]	Shogren A J, Tank J L, Andruszkiewicz E, et al. Controls on eDNA movement in streams: transport, retention, and resuspension[J]. Scientific Reports, 2017, 7:5065. DOI PMID
[9]	Hebert P D N, Cywinska A, Ball S L, et al. Biological identifications through DNA barcodes[J]. Proceedings of the Royal Society B-Biological Sciences, 2003, 270(1512):313-321. DOI URL
[10]	Yoccoz N G. The future of environmental DNA in ecology[J]. Molecular Ecology, 2012, 21(8): 2031-2038. DOI PMID
[11]	康子清, 张银龙, 吴永波, 等. 环境DNA宏条形码在生物多样性研究与监测中的应用[J]. 生物技术通报, 2022, 38(1): 299-310. DOI
[12]	李超伦, 王敏晓, 程方平, 等. DNA条形码及其在海洋浮游动物生态学研究中的应用[J]. 生物多样性, 2011, 19(6): 805-814.
[13]	Strickland G J, Roberts J H. Utility of eDNA and occupancy models for monitoring an endangered fish across diverse riverine habitats[J]. Hydrobiologia, 2019, 826(1): 129-144. DOI
[14]	Sales N G, Wangensteen O S, Carvalho D C, et al. Space-time dynamics in monitoring neotropical fish communities using eDNA metabarcoding[J]. Science of the Total Environment, 2021, 754: 142096. DOI URL
[15]	Zhang S, Lu Q, Wang Y Y, et al. Assessment of fish communities using environmental DNA: effect of spatial sampling design in lentic systems of different sizes[J]. Molecular Ecology Resources, 2020, 20(1): 242-255. DOI PMID
[16]	Wu P, Dutkiewicz S, Monier E, et al. Bottom-heavy trophic pyramids impair methylmercury biomagnification in the marine plankton ecosystems[J]. Environmental Science & Technology, 2021, 55(22): 15476-15483. DOI URL
[17]	Yao M, Zhang S, Lu Q, et al. Fishing for fish environmental DNA: ecological applications, methodological considerations, surveying designs, and ways forward[J]. Molecular Ecology, 2022, 31(20): 5132-5164. DOI URL
[18]	Rees H C, Maddison B C, Middleditch D J, et al. Review the detection of aquatic animal species using environmental DNA - a review of eDNA as a survey tool in ecology[J]. Journal of Applied Ecology, 2014, 51(5): 1450-1459. DOI URL
[19]	Shu L, Ludwig A, Peng Z. Standards for methods utilizing environmental DNA for detection of fish species[J]. Genes, 2020, 11(3):296. DOI URL
[20]	Deiner K, Walser J C, Machler E, et al. Choice of capture and extraction methods affect detection of freshwater biodiversity from environmental DNA[J]. Biological Conservation, 2015, 183: 53-63. DOI URL
[21]	Eichmiller J J, Miller L M, Sorensen P W. Optimizing techniques to capture and extract environmental DNA for detection and quantification of fish[J]. Molecular Ecology Resources, 2016, 16(1): 56-68. DOI PMID
[22]	Cloern J E, Foster S Q, Kleckner A E. Phytoplankton primary production in the world’s estuarine-coastal ecosystems[J]. Biogeosciences, 2014, 11(9): 2477-2501. DOI URL
[23]	陶敏, 王永明, 谢碧文, 等. 沱江浮游生物群落时空分布及相关环境因子分析[J]. 水生生物学报, 2016, 40(2): 301-312.
[24]	Arrigo K R. Marine microorganisms and global nutrient cycles[J]. Nature, 2005, 437(7057): 349-355. DOI
[25]	Zheng H, Wang R, Yu Z, et al. Automatic plankton image classification combining multiple view features via multiple kernel learning[J]. BMC Bioinformatics, 2017, 18(Suppl 16): 570. DOI PMID
[26]	Henson S A, Cael B B, Allen S R, et al. Future phytoplankton diversity in a changing climate[J]. Nature Communications, 2021, 12(1): 5372.
[27]	Branco P, Egas M, Hall S R, et al. Why do phytoplankton evolve large size in response to grazing?[J]. American Naturalist, 2020, 195(1): 20-37.
[28]	Schlüter L, Lohbeck K T, Gröger J P, et al. Long-term dynamics of adaptive evolution in a globally important phytoplankton species to ocean acidification[J]. Science Advances, 2016, 2(7): e1501660.
[29]	柴毅, 彭婷, 郭坤, 等. 2012年夏季长湖浮游植物群落特征及其与环境因子的关系[J]. 植物生态学报, 2014, 38(8): 857-867. DOI
[30]	Nong X, Shao D, Shang Y, et al. Analysis of spatio-temporal variation in phytoplankton and its relationship with water quality parameters in the South-to-North Water Diversion Project of China[J]. Environmental Monitoring and Assessment, 2021, 193(9): 593.
[31]	Chen S N, Shang P L, Kang P L, et al. Metabolic functional community diversity of associated bacteria during the degradation of phytoplankton from a drinking water reservoir[J]. International Journal of Environmental Research and Public Health, 2020, 17(5):1687. DOI URL
[32]	张丽娟, 徐杉, 赵峥, 等. 环境DNA宏条形码监测湖泊真核浮游植物的精准性[J]. 环境科学, 2021, 42(2): 796-807.
[33]	张莉, 张远, 林佳宁, 等. 基于多个扩增子的DNA metabarcoding技术探究黄海微型真核浮游植物多样性[J]. 环境科学, 2019, 40(9): 4052-4060.
[34]	刘卫东, 宋伦, 吴景. 环境样本中微型和微微型浮游植物高通量测序的引物优化[J]. 生态学报, 2017, 37(12): 4208-4216.
[35]	王晨, 陶孟, 李爱民, 等. 基于环境DNA宏条形码技术的秦淮河生物多样性研究[J]. 生态学报, 2022, 42(2): 611-624.
[36]	Kim D K, Park K, Jo H, et al. Comparison of water sampling between environmental DNA metabarcoding and conventional microscopic identification: a case study in Gwangyang Bay, South Korea[J]. Applied Sciences-Basel, 2019, 9(16):3272.
[37]	王靖淇, 王书平, 张远, 等. 高通量测序技术研究辽河真核浮游藻类的群落结构特征[J]. 环境科学, 2017, 38(4): 1403-1413.
[38]	Questel J M, Hopcroft R R, De Hart H M, et al. Metabarcoding of zooplankton diversity within the Chukchi Borderland, Arctic Ocean: improved resolution from multi-gene markers and region-specific DNA databases[J]. Marine Biodiversity, 2021, 51:1-19. DOI
[39]	张曼, 于佳骏, 孙丽丽, 等. 大冶湖和朱婆湖中微型和超微型真核浮游生物多样性研究[J]. 水生态学杂志, 2022, 43(1): 79-85.
[40]	Malviya S, Scalco E, Audic S, et al. Insights into global diatom distribution and diversity in the world's ocean[J]. Proceedings of the National Academy of Sciences of the United States of America, 2016, 113(11): E1516-E1525.
[41]	郭婷, 付智豪, 周春花, 等. 基于环境DNA宏条形码的鄱阳湖真核浮游植物多样性研究[J/OL]. 水生态学杂志:1-12[2023-02-17].https://doi.org/10.15928/j.1674-3075.202112110408.
[42]	Amaral D C, Dunck B, Braghin L S M, et al. Predation by an omnivorous fish and food availability alter zooplankton functional diversity: a microcosm approach[J]. Anais da Academia Brasileira de Ciencias, 2021, 93(Suppl 3): e20200778. DOI URL
[43]	Pinger C, Copeman L, Stowell M, et al. Rapid measurement of total lipids in zooplankton using the sulfo-phospho-vanillin reaction[J]. Analytical Methods, 2022, 14(27): 2665-2672. DOI PMID
[44]	Steinberg D K, Landry M R. Zooplankton and the ocean carbon cycle[J]. Annual Review of Marine Science, 2017, 9: 413-444. DOI PMID
[45]	Jones N T, Gilbert B. Changing climate cues differentially alter zooplankton dormancy dynamics across latitudes[J]. Journal of Animal Ecology, 2016, 85(2): 559-569. DOI PMID
[46]	Arenas-Sánchez A, López-Heras I, Nozal L, et al. Effects of increased temperature, drought, and an insecticide on freshwater zooplankton communities[J]. Environmental Toxicology and Chemistry, 2019, 38(2): 396-411. DOI PMID
[47]	Johnson C L, Runge J A, Curtis K A, et al. Biodiversity and ecosystem function in the gulf of maine: pattern and role of zooplankton and pelagic nekton[J]. PLoS One, 2011, 6(1): e16491.
[48]	高养春, 李海涛, 王孝程, 等. 利用宏DNA条形码研究浮游动物多样性——以鸭绿江口为例[J]. 生态学报, 2020, 40(11): 3822-3832.
[49]	杨佳, 周健, 秦伯强, 等. 太湖梅梁湾浮游动物群落结构长期变化特征(1997—2017年)[J]. 环境科学, 2020, 41(3): 1246-1255.
[50]	Jansson A, Klais-Peets R, Griniene E, et al. Functional shifts in estuarine zooplankton in response to climate variability[J]. Marine Ecology Progress Series, 2020, 10(20): 11591-11606.
[51]	Hébert M P, Fugère V, Beisner B E, et al. Widespread agrochemicals differentially affect zooplankton biomass and community structure[J]. Ecological Applications, 2021, 31(7): e02423.
[52]	张莉, 林佳宁, 张远, 等. ITS高通量测序研究黄海微型真核浮游生物多样性及分布特征[J]. 环境科学, 2018, 39(5): 2368-2379.
[53]	高旭, 杨江华, 张效伟. 浮游动物DNA宏条形码标志基因比较研究[J]. 生态毒理学报, 2020, 15(2): 61-70.
[54]	于文波, 王庆, 魏南, 等. 基于DNA条形码技术的浮游动物休眠卵种类鉴定:以洞庭湖流域常德柳叶湖为例[J]. 湖泊科学, 2020, 32(1): 154-163.
[55]	梁东, 夏军, 宋进喜, 等. 基于eDNA技术的渭河浮游动物多样性及关键种生态位特征[J]. 环境科学, 2021, 42(10): 4708-4716.
[56]	Wangensteen O S, Palacin C, Guardiola M, et al. DNA metabarcoding of littoral hard-bottom communities: high diversity and database gaps revealed by two molecular markers[J]. PeerJ, 2018, 6:e4705. DOI URL
[57]	孙晶莹, 杨江华, 张效伟. 环境DNA(eDNA)宏条形码技术对枝角类浮游动物物种鉴定及其生物量监测研究[J]. 生态毒理学报, 2018, 13(5): 76-86.
[58]	Leray M, Yang J Y, Meyer C P, et al. A new versatile primer set targeting a short fragment of the mitochondrial COⅠregion for metabarcoding metazoan diversity: application for characterizing coral reef fish gut contents[J]. Frontiers in Zoology, 2013, 10(1): 1-14. DOI URL
[59]	Ankley P J, Xie Y, Havens S, et al. RNA metabarcoding helps reveal zooplankton community response to environmental stressors[J]. Environmental Pollution, 2022, 292: 118446. DOI URL
[60]	王方晗, 王雷, 孙婷婷, 等. 基于形态学和宏条形码技术的南海西沙群岛浮游动物多样性的比较分析[J]. 应用海洋学学报, 2022, 41(2): 317-327.
[61]	唐晟凯, 钱胜峰, 沈冬冬, 等. 应用环境DNA技术对邵伯湖浮游动物物种检测的初步研究[J] 水产养殖[J]. 2021, 42(3): 13-20.
[62]	Benson D A, Cavanaugh M, Clark K, et al. GenBank[J]. Nucleic Acids Research, 2018, 46(D1): D41-D47.
[63]	Porter T M, Hajibabaei M. Over 2.5 million COⅠsequences in GenBank and growing[J]. PLoS One, 2018, 13(9): e0200177.
[64]	陈岩, 张立, 刘力, 等. 我国检疫性有害生物DNA条形码信息系统建设[J] 植物检疫. 2014, 28(1): 1-5.
[65]	Kim S, Kim C B, Min G S, et al. Korea barcode of life database system (KBOL)[J]. Animal Cells and Systems, 2012, 16(1): 11-19. DOI URL
[66]	Carew M E, Nichols S J, Batovska J, et al. A DNA barcode database of Australia’s freshwater macroinvertebrate fauna[J]. Marine and Freshwater Research, 2017, 68(10): 1788-1802. DOI URL
[67]	王萌, 苑艺, 于海燕, 等. 中国淡水大型底栖无脊椎动物条形码数据库构建[J]. 中国环境监测, 2022, 38(1): 36-44.

条形码基因 Barcode genes	长度/bp Length	引物序列 Primer sequences	应用示例 Application examples
18S rRNA -V9	130	F:TCCCTGCCHTTTGTACACAC	研究湖泊真核浮游植物^[32]
18S rRNA -V9	130	R:CCTTCYGCAGGTTCACCTAC	研究湖泊真核浮游植物^[32]
18S rRNA -V9	130	F:CCCTGCCHTTTGTACACAC	探究黄海微型真核浮游植物多样性^[33]
18S rRNA -V9	130	R:CCTTCYGCAGGTTCACCTAC	探究黄海微型真核浮游植物多样性^[33]
18S rRNA-V9	130	F:TCCCTGCCHTTTGTACACAC	研究秦淮河生物多样性^[35]
18S rRNA-V9	130	R:CCTTCYGCAGGTTCACCTAC	研究秦淮河生物多样性^[35]
18S rRNA-V9	130	F:CCCTGCCHTTTGTACACAC R:CCTTCYGCAGGTTCACCTAC	研究韩国蟾津江入海口光阳湾生物多样性^[36]
18S rRNA-V4	370	F:GCGGTAATTCCAGCTCCAATA	筛选出适合研究微型和微微型浮游植物的引物^[34]
18S rRNA-V4	370	R:GATCCCCHWACTTTCGTTCTTGA	筛选出适合研究微型和微微型浮游植物的引物^[34]
18S rRNA-V4	500	F:GGCAAGTCTGGTGCCAG	研究辽河真核浮游藻类的群落结构特征^[37]
18S rRNA-V4	500	R:GACTACGACGGTATCTRATCRTCTTCG	研究辽河真核浮游藻类的群落结构特征^[37]
18S rRNA-V4	450	F:CCAGCASCYGCGGTAATTCC	研究北冰洋浮游生物多样性^[38]
18S rRNA-V4	450	R:ACTTTCGTTCTTGATYRR	研究北冰洋浮游生物多样性^[38]
18S rRNA-V4	380	F:CCAGCASCYGCGGTAATTCC	研究湖泊中微型和超微型真核浮游生物多样性^[39]
18S rRNA-V4	380	R:ACTTTCGTTCTTGATYRA	研究湖泊中微型和超微型真核浮游生物多样性^[39]

条形码基因 Barcode genes	长度/bp Length	引物序列 Primer sequences	应用示例 Application examples
18S rRNA -V9	130	F:TCCCTGCCHTTTGTACACAC	研究湖泊真核浮游植物^[32]
18S rRNA -V9	130	R:CCTTCYGCAGGTTCACCTAC	研究湖泊真核浮游植物^[32]
18S rRNA -V9	130	F:CCCTGCCHTTTGTACACAC	探究黄海微型真核浮游植物多样性^[33]
18S rRNA -V9	130	R:CCTTCYGCAGGTTCACCTAC	探究黄海微型真核浮游植物多样性^[33]
18S rRNA-V9	130	F:TCCCTGCCHTTTGTACACAC	研究秦淮河生物多样性^[35]
18S rRNA-V9	130	R:CCTTCYGCAGGTTCACCTAC	研究秦淮河生物多样性^[35]
18S rRNA-V9	130	F:CCCTGCCHTTTGTACACAC R:CCTTCYGCAGGTTCACCTAC	研究韩国蟾津江入海口光阳湾生物多样性^[36]
18S rRNA-V4	370	F:GCGGTAATTCCAGCTCCAATA	筛选出适合研究微型和微微型浮游植物的引物^[34]
18S rRNA-V4	370	R:GATCCCCHWACTTTCGTTCTTGA	筛选出适合研究微型和微微型浮游植物的引物^[34]
18S rRNA-V4	500	F:GGCAAGTCTGGTGCCAG	研究辽河真核浮游藻类的群落结构特征^[37]
18S rRNA-V4	500	R:GACTACGACGGTATCTRATCRTCTTCG	研究辽河真核浮游藻类的群落结构特征^[37]
18S rRNA-V4	450	F:CCAGCASCYGCGGTAATTCC	研究北冰洋浮游生物多样性^[38]
18S rRNA-V4	450	R:ACTTTCGTTCTTGATYRR	研究北冰洋浮游生物多样性^[38]
18S rRNA-V4	380	F:CCAGCASCYGCGGTAATTCC	研究湖泊中微型和超微型真核浮游生物多样性^[39]
18S rRNA-V4	380	R:ACTTTCGTTCTTGATYRA	研究湖泊中微型和超微型真核浮游生物多样性^[39]

条形码基因 Barcode genes	长度/bp Length	引物序列 Primer sequences	应用示例 Application examples
COⅠ	313	F:WACWGGWTGAACWGTWTAYCCYCC	筛选出更适合研究浮游动物 DNA 宏条形码引物^[53]
COⅠ	313	R:TAAACTTCAGGGTGACCAAARAAYCA	筛选出更适合研究浮游动物 DNA 宏条形码引物^[53]
COⅠ	116	F:TTAGGRGCHCCWGAYATRGCTT	鉴定枝角类浮游动物物种,并研究其生物量^[57]
COⅠ	116	R: GCRTGRGCRATHCCHGCWGA	鉴定枝角类浮游动物物种,并研究其生物量^[57]
COⅠ	310	F:GGWACWGGWTGAACWGTWTAYCCYCC	研究秦淮河生物多样性^[35]
COⅠ	310	R:GGRGGRTASACSGTTCASCCSGTSCC	研究秦淮河生物多样性^[35]
COⅠ	649	F:TGTAAAACGACGGCCAGTTCTASWAAT	鉴定浮游动物休眠卵种类^[54]
COⅠ	649	R:CAGGAAACAGCTATGACTTCAGGRTGR	鉴定浮游动物休眠卵种类^[54]
COⅠ	313	F:GGWACWGGWTGAACWGTWTAYCCYCC	大规模生物多样性评估^[58]
COⅠ	313	R:TAIACYTCIGGRTGICCRAARAAYCA	大规模生物多样性评估^[58]
COⅠ	313	F:GGWACWGGWTGAACWGTWTAYCCYCC R:TAAACTTCAGGGTGACCAAAAAATCA	研究加拿大安大略省58个湖泊的浮游动物^[59]
18S rRNA-V4	380	F:CCAGCASCYGCGGTAATTCC	研究湖泊中微型和超微型真核浮游生物多样性^[39]
18S rRNA-V4	380	R:ACTTTCGTTCTTGATYRA	研究湖泊中微型和超微型真核浮游生物多样性^[39]
18S rRNA-V4	370	F:AGGGCAAKYCTGGTGCCAG	研究鸭绿江口浮游动物多样性^[48]
18S rRNA-V4	370	R:GRCGGTATCTRATCGYCTT	研究鸭绿江口浮游动物多样性^[48]
18S rRNA-V9	130	F:GTACACACCGCCCGTC	分析南海西沙群岛浮游动物多样性^[60]
18S rRNA-V9	130	R:TGATCCTTCTGCAGGTTCACCTAC	分析南海西沙群岛浮游动物多样性^[60]

条形码基因 Barcode genes	长度/bp Length	引物序列 Primer sequences	应用示例 Application examples
COⅠ	313	F:WACWGGWTGAACWGTWTAYCCYCC	筛选出更适合研究浮游动物 DNA 宏条形码引物^[53]
COⅠ	313	R:TAAACTTCAGGGTGACCAAARAAYCA	筛选出更适合研究浮游动物 DNA 宏条形码引物^[53]
COⅠ	116	F:TTAGGRGCHCCWGAYATRGCTT	鉴定枝角类浮游动物物种,并研究其生物量^[57]
COⅠ	116	R: GCRTGRGCRATHCCHGCWGA	鉴定枝角类浮游动物物种,并研究其生物量^[57]
COⅠ	310	F:GGWACWGGWTGAACWGTWTAYCCYCC	研究秦淮河生物多样性^[35]
COⅠ	310	R:GGRGGRTASACSGTTCASCCSGTSCC	研究秦淮河生物多样性^[35]
COⅠ	649	F:TGTAAAACGACGGCCAGTTCTASWAAT	鉴定浮游动物休眠卵种类^[54]
COⅠ	649	R:CAGGAAACAGCTATGACTTCAGGRTGR	鉴定浮游动物休眠卵种类^[54]
COⅠ	313	F:GGWACWGGWTGAACWGTWTAYCCYCC	大规模生物多样性评估^[58]
COⅠ	313	R:TAIACYTCIGGRTGICCRAARAAYCA	大规模生物多样性评估^[58]
COⅠ	313	F:GGWACWGGWTGAACWGTWTAYCCYCC R:TAAACTTCAGGGTGACCAAAAAATCA	研究加拿大安大略省58个湖泊的浮游动物^[59]
18S rRNA-V4	380	F:CCAGCASCYGCGGTAATTCC	研究湖泊中微型和超微型真核浮游生物多样性^[39]
18S rRNA-V4	380	R:ACTTTCGTTCTTGATYRA	研究湖泊中微型和超微型真核浮游生物多样性^[39]
18S rRNA-V4	370	F:AGGGCAAKYCTGGTGCCAG	研究鸭绿江口浮游动物多样性^[48]
18S rRNA-V4	370	R:GRCGGTATCTRATCGYCTT	研究鸭绿江口浮游动物多样性^[48]
18S rRNA-V9	130	F:GTACACACCGCCCGTC	分析南海西沙群岛浮游动物多样性^[60]
18S rRNA-V9	130	R:TGATCCTTCTGCAGGTTCACCTAC	分析南海西沙群岛浮游动物多样性^[60]

环境DNA宏条形码在浮游动植物多样性研究中的应用

Application of environmental DNA metabarcoding in studying biodiversity of zooplankton and phytoplankton

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

图/表 2

参考文献 67

相关文章 10

编辑推荐

Metrics

[1]	游剑涛. 三沙湾及邻近开放海域夏季浮游植物群落结构比较[J]. 渔业研究, 2022, 44(6): 581-587.
[2]	刘勇马超赵文武翁祖桐徐春燕庄之栋蔡建堤谢少卿沈长春. 闽江口及附近海域春夏季鱼类群落结构特征[J]. 渔业研究, 2022, 44(5): 467-476.
[3]	邹双燕. 平潭近岸海域春、夏季浮游植物特征及其与环境因子相关性分析[J]. 渔业研究, 2022, 44(3): 266-274.
[4]	齐喜荣. 汉江上游渔业资源调查[J]. 渔业研究, 2022, 44(1): 21-32.
[5]	李希磊于潇杨俊丽崔龙波. 山东莱州一例扇贝保苗池塘水环境质量评价分析[J]. , 2018, 40(3): 186-195.
[6]	张光星吴钟解陈石泉蔡泽富王道儒. 春季三亚湾浮游动物生态特征与环境因子的关系[J]. 福建水产, 2014, 36(3): 176-184.
[7]	郑惠东. 福建南日岛周围海域浮游动物的种类组成与数量分布特征[J]. , 2014, 36(3): 185-190.
[8]	张玉荣丁跃平郭远明李铁军薛彬鲍静娇朱剑. 乐清湾浮游植物群落结构调查研究[J]. 福建水产, 2013, 35(4): 249-257.
[9]	肖莹. 闽江口海域浮游植物群落结构特征[J]. 福建水产, 2013, 35(4): 258-263.
[10]	张玉荣. 乐清湾浮游动物现状调查研究[J]. 福建水产, 2012, 34(3): 189-195.