急性低氧胁迫对四指马鲅幼鱼鳃和肝组织损伤的影响

doi:10.14012/j.cnki.fjsc.2023.01.003

渔业研究 ›› 2023, Vol. 45 ›› Issue (1): 14-22.DOI: 10.14012/j.cnki.fjsc.2023.01.003

急性低氧胁迫对四指马鲅幼鱼鳃和肝组织损伤的影响

林欣¹^,²(), 区又君²^,³^,^*(), 温久福²^,³, 徐淑玉²^,⁴, 李加儿²^,³

1.大连海洋大学水产与生命学院,辽宁大连 116000
2.中国水产科学研究院南海水产研究所,农业农村部南海渔业资源开发利用重点实验室,广东广州 510300
3.岭南现代农业科学与技术广东省实验室茂名分中心,广东茂名 525000
4.上海海洋大学水产与生命学院,上海 201306

收稿日期:2022-09-14 出版日期:2023-02-25 发布日期:2023-02-21
通讯作者: 区又君(1964-),女,研究员,从事鱼类生物学、发育生物学与水产增养殖技术研究。E-mail:ouyoujun@126.com
作者简介:林欣(1998-)女,硕士研究生,研究方向为鱼类生物学。E-mail:1600662461@qq.com
基金资助:
国家重点研发计划“蓝色粮仓科技创新”重点专项(2018YFD0900200);中国水产科学研究院基本科研业务费资助项目(2020TD55);岭南现代农业科学与技术广东省实验室茂名分中心基金资助课题(2022ZD009)

Effects of acute hypoxic stress on Eleutheronema tetradactylum gill organ and liver tissue damage

LIN Xin¹^,²(), OU Youjun²^,³^,^*(), WEN Jiufu²^,³, XU Shuyu²^,⁴, LI Jia'er²^,³

1. College of Fisheries and Life Science, Dalian Ocean University, Dalian 116000, China
2. Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China
3. Maoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture,Maoming 525000,China
4. College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China

Received:2022-09-14 Online:2023-02-25 Published:2023-02-21

摘要/Abstract

摘要：

为探究急性低氧胁迫对四指马鲅(Eleutheronema tetradactylum)幼鱼鳃器官的影响以及肝脏组织损伤情况,对四指马鲅进行了水体溶解氧为2 mg/L、时间为120 min的急性低氧胁迫试验,采用常规石蜡切片和 HE 染色以及电子显微技术,观察四指马鲅的鳃和肝脏器官。结果显示,在急性低氧胁迫下,四指马鲅鳃丝内血窦收缩、末端膨大,鳃小片长度变长、间隔变大,部分上皮细胞肿胀凸起;线粒体丰富细胞的顶隐窝由内凹变为平整,胞内线粒体形态由椭圆形变为圆形;肝组织出现肝细胞坏死、肝细胞融合等现象。表明低氧胁迫对四指马鲅鳃和肝组织具有较大的影响,且120 min就会造成组织结构的损伤。

关键词: 四指马鲅, 低氧胁迫, 鳃, 肝, 组织学

Abstract:

To investigate the effects of acute hypoxic stress on the gill organs and liver tissue damage of juvenile Eleutheronema tetradactylum, a 2 h acute hypoxic stress experiment was conducted with 2 mg/L of dissolved oxygen in the water. Then the gills and liver organs of E.tetradactylum were observed using conventional paraffin sections and HE staining as well as electron microscopic techniques. The results displayed contraction of the blood sinuses in the gill filaments of E.tetradactylum under acute hypoxic stress, with dilated ends; longer gill lamellae, larger intervals and swollen and bulging parts of the epithelium. The parietal crypt of mitochondria-rich cells changed from concave to flat and the intracellular mitochondrial morphology changes from oval to round. Liver tissue showed hepatocyte necrosis and fusion of hepatocytes. Hypoxic stress had a large effect on the gill and liver tissues of E.tetradactylum and caused structural damage to the tissues by 2 h.

Key words: Eleutheronema tetradactylum, gill, liver, hypoxia stress, histology

中图分类号:

S917.4

林欣, 区又君, 温久福, 徐淑玉, 李加儿. 急性低氧胁迫对四指马鲅幼鱼鳃和肝组织损伤的影响[J]. 渔业研究, 2023, 45(1): 14-22.

LIN Xin, OU Youjun, WEN Jiufu, XU Shuyu, LI Jia'er. Effects of acute hypoxic stress on Eleutheronema tetradactylum gill organ and liver tissue damage[J]. Journal of Fisheries Research, 2023, 45(1): 14-22.

图/表 4

图1 四指马鲅幼鱼鳃显微结构注:a、c为对照组(400X);b、d为2 mg/L低氧组(400X);GL:鳃丝;SL:鳃小片;BC:血细胞;PVC:扁平上皮细胞;MRC:线粒体丰富细胞;SC:柱细胞;▲:肿胀;★:破裂。

Fig.1 Microstructure of the gills of E. tetradactylum Notes:a and c were control group(400X);b and d were 2 mg/L dissolved oxygen group(400X);GL.Gill filament;SL.Secondary gill lamella;BC.Blood cell;PVC.Pavement cell;MRC.Mitochondria rich cell;SC.Pillar cell;▲.Swell;★.Rupture.

表1 低氧胁迫下四指马鲅幼鱼鳃小片的变化

Tab.1 The changes of secondary gill lamella of E.tetradactylum juveniles under hypoxic stress

组别 Group	溶解氧浓度/ (mg/L) Dissolved oxygen concentration	鳃小片间隔/μm Space between secondary gill lamella	鳃小片长度/μm Length of secondary gill lamella	鳃小片宽度/μm Width of secondary gill lamella
低氧组 Hypoxia group	2.0	18.272±0.41^a	49.288±4.86^a	3.558±0.66^a
对照组 Control group	6.0	12.993±1.89^b	31.916±3.54^b	7.071±1.29^b

图2 指马鲅幼鱼鳃超微结构（透射电镜）注:a、c、e为对照组; b、d、f为2 mg/L低氧组;EP.上皮细胞;M.线粒体;MRC.线粒体丰富细胞;BC.血细胞;★.顶隐窝;Pc:柱细胞;N:细胞核。

Fig.2 Ultrastructure of the gills of E. tetradactylum (TEM) Notes:a,c,e were control group;b,d,f were 2 mg/L dissolved oxygen group;EP.Epithelial cell;M.Mitochondria;MRC.Mitochondria rich cell;BC.Blood cell;★.Top crypt;Pc.Pillar cell;N.Nucleus.

图3 四指马鲅幼鱼肝脏显微结构注:a、c为对照组(400X);b、d为2 m/L溶氧组(400X);BV:血管;BC:血细胞;HC:肝细胞;HN:肝细胞核;HS:肝血窦;★:肝细胞融合;▲肝细胞坏死。

Fig.3 Microstructure of the liver of E. tetradactylum Notes:a,c were control group(400X);b,d were 2 mg/L dissolved oxygen group(400X);BV.Blood vessel;BC.Blood cell;HC.Hepatocytes;HN.Hepatocyte nucleus;HS.Hepatic sinusoid;★.Hepatocyte fusion;▲.Hepatocyte necrosis.

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急性低氧胁迫对四指马鲅幼鱼鳃和肝组织损伤的影响

Effects of acute hypoxic stress on Eleutheronema tetradactylum gill organ and liver tissue damage

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