Abstract:

In order to understand the physiological characteristics of sperm of Percocypris pingi, the variation of the sperm vitality of P. pingi in different NaCl concentration conditions was observed in this study. The results indicated that: the sperm of P. pingi could be activated in the solution with the concentrations of NaCl ranged from 0% to 0.65%. When the NaCl concentration was 0.35%, the P. pingi spermatozoa intense exercise time and the lifespan were (66.33±7.99) s and (635.66±95.78) s, respectively. At this concentration, the life-span was significantly longer than the sperm activated in the other treatment groups (P<0.01). The time of intense exercise was longer than the sperm activated in the 0.60% group and 0.65% group (P<0.05), but there was no significant difference with the other treatment groups. When the concentration of NaCl exceeded 0.35%, the activity of sperm decreased and the life span was shortened. The spermatozoa could not be activated when the concentration of NaCl solution exceeded 0.70%. This study would provide reference for sperm preservation of the P. pingi.