关键词: 呕吐毒素, 解淀粉芽孢杆菌, 生物解毒酶, 生物活性剂T, 解毒效率, vomiting toxin, Bacillus amyloliquefaciens, biological detoxification enzyme, biologically active agent T, detoxification efficiency

Abstract: Vomitoxin is a natural mold product, a major pollutant in feed and its raw materials, and a mycotoxin that has appeared most frequently in aquatic feed in recent years. In this study, a certain amount of Bacillus amyloliquefaciens (106 , 107 , 108 CFU/mL) , biological detoxifying enzymes, and biologically active agent T were used for combination compatibility. Under the condition of 28℃, the detoxifying efficiency of different combinations of compatibility on vomiting toxin was tested by enzyme - linked immunosorbent assay (ELISA) after in vitro culture for 16 h. The results showed that Bacillus amyloliquefaciens, biological detoxification enzyme and biologically active agent T all had a certain detoxification efficiency against the vomiting toxin. Among them, the detoxification efficiency of Bacillus amyloliquefaciens and biological detoxification enzymes combination was better，and the detoxification efficiency was the highest in 106 CFU/mL Bacillus amyloliquefaciens.