关键词: 呋喃丹, 超高效液相色谱, 全自动固相萃取, 淡水养殖水体, carbofuran, ultra high performance liquid chromatography, automatic solid phase extraction, freshwater aquaculture water

Abstract: A new method was developed for the determination of carbofuran in freshwater aquaculture water by ultra high pressure liquid chromatography (UPLC) combined with automatic solid phase extraction (SPE). The water sample flowed through HLB SPE cartridge with a flow rate of 5 mL·min-1, and the analyte was eluted with 10 mL methanol in SPE cartridge. After concentrating and redissolving with acetonitrile-water (40∶60, V/V), the eluent was analyzed by ultra high performance liquid chromatography with fluorescence detection. The analysis conditions were as the follows: chromatographic column: ACQUITY UPLC BEH C18 (100 mm × 2.1 mm, 1.7 μm), mobile phase: acetonitrile-water (40∶60, V/V), flow rate: 0.200 mL·min-1, excitation wavelength: 270 nm, emission wavelength: 310 nm. The correlation coefficients of carbofuran standard curve was more than 0.999 in the range of 20 ~ 1000 μg·L-1. The limit of detection was 0.10 μg·L-1 and the limit of quantitation was 0.25 μg·L-1. The average recoveries were in the range of 90.4% ~ 96.3% and the relative standard deviations were 2.55% ~ 5.10% at 0.25 μg/L, 2.50 μg·L-1 and 10.0 μg·L-1 spiked levels. The method is highly automated, simple, rapid, accurate and stable, and is suitable for the determination of carbofuran in freshwater aquaculture water.