关键词: 中华绒螯蟹, 氟苯尼考, 代谢动力学, 温度, Eriocheir sinensis, florfenicol, pharmacokinetics, temperature

Abstract: A pharmacokinetic study of florfenicol following a single intramuscular administration (20 mg?kg-1) was carried out in the Chinese mitten-handed crab at 17℃ and 28℃ water temperature. Hemolymph, muscle and liver samples were collected at different time after injection. The tissue level of flofenicol was determined by high-performance liquid chromatography method. The florfenicol level in hemolymph and muscle reached maximum momentarily, while that in liver increased firstly and reduced at last with administration time. The pharmacokinetics of florfenicol in the Chinese mitten-handed crab was conformed to a two-compartment model. At 17℃ and 28℃, the absorption half-life, elimination half-life and the area under the concentration-time curve from zero to infinity of florfenicol in liver were found to have a negative correlation with temperature ,whereas total body clearance revealed a positive correlation.