高效液相色谱法检测鲈鱼中已烯雌酚含量

福建水产 ›› 2013, Vol. 35 ›› Issue (2): 124-128.

高效液相色谱法检测鲈鱼中已烯雌酚含量

张振霖

厦门市海洋与渔业研究所

收稿日期:2013-01-15 修回日期:2013-02-06 出版日期:2013-04-25 发布日期:2013-04-27
通讯作者: 张振霖
基金资助:
水产品中激素已烯雌酚残留的快速检测方法研究与应用

Rapid Determination of Diethylstilbestrol in perch by High Performance Liquid Chromatography

Received:2013-01-15 Revised:2013-02-06 Online:2013-04-25 Published:2013-04-27

摘要/Abstract

摘要： 本文建立了检测鲈鱼中已烯雌酚含量的高效液相色谱法，实验结果表明，该方法对已烯雌酚标准品的线性回归方程相关系数为0.9999，线性范围为0.00~2.00 μg/mL，最低检测限为0.06 μg/mL，已烯雌酚添加量为0.04-0.80 mg/kg时，加标回收率为78.00%-82.30%，变异系数为0.25%-1.37%，说明该方法精密度好、灵敏度高, 结果可靠, 可满足快速准确检测鲈鱼中已烯雌酚残留的需求。

关键词: 高效液相色谱鲈鱼己烯雌酚检测, High Performance Liquid Chromatography Perch Diethylstilbestrol Determination

Abstract: A method has been developed for rapid determination of diethylstilbestrol in perch by high performance liquid chromatography. The results showed that the calibration coefficient is 0.9999, the dynamic linear range is 0.00-2.00μg/mL, The limit of detection is 0.06μg/mL, The average recovery is 78.00 ％-82.30, and their relative standard deviation is 0.25%-1.37%. The method can be used to rapid determination of diethylstilbestrol in perch with satisfactory precision , sensitivity and accuracy.

张振霖. 高效液相色谱法检测鲈鱼中已烯雌酚含量[J]. 福建水产, 2013, 35(2): 124-128.