关键词: 网箱养殖, 盐硫杆菌属, 分离鉴定, 硫代硫酸盐, 生长特性, cage culture, Halothiobacillns sp., isolation and identification , thiosulfate, growth characteristics

Abstract: Screening sulfide-oxidizing bacteria from sediments of marine cage culture area, then exploring the growth characterization of the bacteria. The medium with sodium thiosulfate as the sole energy substrate was used to isolate a sulfide - oxidizing bacterium. The species of the strain were identified by a variety of methods of Gram staining, plate colony morphologic observation, morphological characteristics research,16S rRNA gene sequence analysis and the molecular phylogenetic tree construction. An efficient thiosulphate-oxidizing bacterium was isolated from Bachimen of Fujian, named B1 - 1. The strain was Gram–negative bacteria, short rod and identified as Halotm obacillns hydrothermalis（GenBank accession no.KU362926).Under the culture conditions of 28℃， pH 8and 150 r/min， the phases of logarithmic of strain B1-1 had come in 12 h， then the thiosulfate was oxidated in 36 h，the medium pH was down to 4.8 and the concentration of S2O3- was reduced from initial 2.28 g/L to 0.36 g/L in 60 h. The strain of sodium thiosulfate oxidation was up to 84.2 %，which showed great application prospect.