关键词: 刺激隐核虫, PCR检测, ITS, Cryptocaryon irritans, PCR detection, internal transcribed spacer

Abstract: Specific primers were designed based on the DNA sequence of internal transcribed spacer 1 (ITS-1). The genomic DNA of Cryptocaryon irritans，isolated from culturing Pseudosciaena crocea in Luoyuan of Fujian，used as a template DNA to amplify part of the gene fragment of ITS-1，which had a 387 bp band when electrophoresed on an agarose gel. Then the PCR amplification products were cloned，sequenced and analyzed. Homology comparison indicated that they shared 100 % identity with the gene sequence of CL1209 strain ( KC550300)，FD 1210 ( KC550302) ， PYH 4.12 strain ( DQ270010) ，Wakayama strain (AB608054)， and Chiayi strain (AF490381) . PCR amplification for the detection of Cryptocaryon irritans was established， which was limited to 2 polypides， then being applied to detect the samples of the C. irritans monitoring during the last three years (2011-2013 ) . The results were 100% conformed to clinical test results. It revealed that this PCR amplification method was quick，sensitive reproducible and applicable for the detection of Cryptocaryon irritans.