鲍活体倍性检测技术的研究

福建水产 ›› 2012, Vol. 34 ›› Issue (3): 225-230.

鲍活体倍性检测技术的研究

林美金

福建省宁德市蕉城区水产技术推广站

收稿日期:2012-03-27 修回日期:2012-04-12 出版日期:2012-06-25 发布日期:2012-06-21
通讯作者: 林美金
基金资助:
国家农业科技成果转化项目

DNA Ploidy Determination technology for Hybrid Abalone (♀Haliotis discus hannai × ♂Haliotis discus discus)

Mei-jin LIN

Received:2012-03-27 Revised:2012-04-12 Online:2012-06-25 Published:2012-06-21
Contact: Mei-jin LIN

摘要/Abstract

摘要： 摘要：在生物有机体中，一个细胞核中所含的DNA总量对于某种类有机体是一定的。利用流式细胞仪（FCM）测量细胞中的DNA含量，可以用来鉴别有机体的倍性。本研究取杂交鲍[即皱纹盘鲍（Haliotis discus hannai）×盘鲍（Haliotis. discus discus）]的一小块触手，制成细胞悬浮液，经DAPI染色后，用PA-Ⅱ型倍体分析仪测定其倍性。试验表明：振荡最适时间为1.5～2.0min，染色最适时间为4.0～5.0min。

关键词: 关键词：鲍倍性检测流式细胞仪 DAPI, Key words: Hybrid abalone（♀ Haliotis discus hannai × ♂Haliotis discus discus） DNA ploidy determination, Flow Cytometry, Destination Access Point Identifier

Abstract: Abstract: The total DNA yield in the cell nucleus vary with different species, the species DNA ploidy can be determined according to the analysis of the DNA content of the cell by Flow Cytometry. In the present study, the DNA ploidy determination for hybrid abalone（♀Haliotis discus hannai ×♂Haliotis discus discus）was studied, cells suspension was provided from small tentacles of hybrid abalone, then conducted staining by Destination Access Point Identifier(DAPI), finally, DNA ploidy determination was finished by PA-Ⅱploidy analyzer. The results showed that the optimal time for oscillation was 1.5～2.0 minutes, the optimal staining time is 4.0～5.0 minutes.

林美金. 鲍活体倍性检测技术的研究[J]. 福建水产, 2012, 34(3): 225-230.

Mei-jin LIN. DNA Ploidy Determination technology for Hybrid Abalone (♀Haliotis discus hannai × ♂Haliotis discus discus)[J]. JOURNAL OF FUJIAN FISHERIES, 2012, 34(3): 225-230.