链状裸甲藻对翡翠贻贝清滤率的影响

doi:10.14012/j.cnki.fjsc.2023.06.005

摘要/Abstract

摘要：

为研究链状裸甲藻(Gymnodinium catenatum)对翡翠贻贝(Perna viridis)摄食的影响,设置试验组和对照组,采用链状裸甲藻和中肋骨条藻(Skeletonema costatum)投喂翡翠贻贝,开展1 h和长期(38 d)清滤率试验。结果表明:翡翠贻贝无论是摄食产毒藻或无毒藻,随着藻细胞密度的升高,清滤率的变化均呈先升高后降低的趋势,峰值出现在藻细胞密度接近赤潮基准密度时。试验组和对照组翡翠贻贝清滤率的最高值分别为(3.04±0.02)、(3.70±0.03)L/(g·h),与对照组相比,试验组翡翠贻贝清滤率显著下降(P<0.05),表明链状裸甲藻对翡翠贻贝的摄食产生了影响。长期滤食链状裸甲藻的翡翠贻贝的清滤率基本保持在初始水平,与短期滤食相比无显著变化。

关键词: 链状裸甲藻, 翡翠贻贝, 清滤率

Abstract:

The present study was conducted to investigate the impact of G.catenatum on the feeding behavior of P.viridis. An experimental group fed with G.catenatum and a control group fed with Skeletonema costatum were established. Short-term (1 hour) and long-term (38 days) clearance rate experiments were carried out. The results showed that the clearance rate of P.viridis, whether feeding on toxic or non-toxic algae, as the densty of algae cells increased, initially increased and then decreased, reaching the peak when the algal cell density approached the threshold density for red tide. The highest clearance rates observed in the experimental and control groups were (3.04±0.02) and (3.70±0.03) L/(g·h), respectively. Compared to the control group, the clearance rate of P.viridis in the experimental group exhibited a significant decrease (P<0.05), indicating that G.catenatum had an influence on the feeding behavior of P.viridis. Furthermore, the long-term filtration did not cause significant changes in the clearance rate, which remained relatively stable at the initial level.

Key words: Gymnodinium catenatum, Perna viridis, clearance rate

中图分类号:

X171

许翠娅, 杨芳, 陈小红, 陈财珍. 链状裸甲藻对翡翠贻贝清滤率的影响[J]. 渔业研究, 2023, 45(6): 551-558.

XU Cuiya, YANG Fang, CHEN Xiaohong, CHEN Caizhen. Effects of Gymnodinium catenatum on the clearance rate of Perna viridis[J]. Journal of Fisheries Research, 2023, 45(6): 551-558.

图/表 5

图1 1 h清滤率试验中试验组清滤率的变化注:G1~G6链状裸甲藻藻液密度分别为2.5×104、5.0×104、1.0×105、2.5×105、5.0×105、1.0×106 cells/L。同一单位下,不同字母表示清滤率差异显著(P<0.05),相同则差异不显著(P>0.05)。下同。

Fig.1 The change of clearance rate of the experimental group in the 1 h clearance rate experiment Notes: The algal density of G.catenatum were 2.5×104, 5.0×104, 1.0×105, 2.5×105, 5.0×105, 1.0×106 cells/L, respectively. Under the same unit,the different letters in the figure indicated that there was a significant difference in the clearance rate(P<0.05),or the same letters meant no difference(P>0.05).The same as below.

图2 1 h清滤率试验中对照组清滤率的变化注:S1~S6中肋骨条藻藻液密度分别为1.0×105、2.5×105、5.0×105、1.0×106、2.5×106和5.0×106 cells/L。

Fig.2 The change of clearance rate of the control group in the 1 h clearance rate experiment Notes:The algal density of S.costatum were 1.0×105, 2.5×105, 5.0×105, 1.0×106 ,2.5×106, 5.0×106 cells/L, respectively.

图3 翡翠贻贝摄食作用下藻液密度的变化注:同种藻类下的显著性分析。

Fig.3 The change of algae density under the feeding of P. viridis Note:Signidicance analysis under the same algae specie.

图4 累积试验中翡翠贻贝清滤率的变化注:同一组别下的显著性分析。

Fig.4 The change of clearance rate of P. viridis in accumulation experiment Note:Significance anaylsis under the same group.

图5 排出试验中翡翠贻贝清滤率的变化注:组内、组间的多重比较。

Fig.5 The change of clearance rate of P. viridis in depuration experiment Note:Multiple comparisons within and between groups.

参考文献 31

[1]	颜天, 傅萌, 刘红, 等. 双壳类清滤率在海洋污染物毒性研究中的应用[J]. 海洋科学, 2003, 27 (1):50-53.
[2]	王朝辉, Matsuoka K, 齐雨藻, 等. 有毒亚历山大藻(Alexandrium spp.)和链状裸甲藻(Gymnodinium catenatum)孢囊在中国沿海的分布[J]. 海洋与湖沼, 2003, 34(4):422-430.
[3]	陈火荣. 2017年福建海域链状裸甲藻赤潮事件应急处置与思考[J]. 渔业研究, 2018, 40(4):308-314.
[4]	焦玥, 田华, 王宗灵, 等. 微小亚历山大藻投喂量与菲律宾蛤仔对麻痹性贝毒累积、转化和排出的动态关系[J]. 海洋科学进展, 2010, 28(2):216-223.
[5]	Shumway S E, Cucci T L. The effects of the toxic dinoflagellate Protogonyaulax tamarensis on the feeding and behaviour of bivalve molluscs[J]. Aquatic Toxicology, 1987, 10(1): 9-27. DOI URL
[6]	Coughlan J. The estimation of filtering rate from the clearance of suspensions[J]. Marine Biology, 1969, 2(4): 356-358. DOI URL
[7]	杨晓新, 林小涛, 计新丽, 等. 温度、盐度和光照条件对翡翠贻贝滤水率的影响[J]. 海洋科学, 2000, 24(6):36-39.
[8]	中华人民共和国国家海洋局.赤潮监测技术规程: HY/T 069—2005[S]. 北京: 中国标准出版社, 2005.
[9]	Ward J E, MacDonald B A, Thompson R J. Mecharusms of suspension feeding in bivalves: resolution of current controversies by means of endoscopy[J]. Limnology and Oceanography, 1993, 38(2): 265-272. DOI URL
[10]	董波, 薛钦昭, 李军. 滤食性贝类摄食生理的研究进展[J]. 海洋科学, 2000, 24(7):31-34.
[11]	包永波, 尤仲杰. 海洋滤食性贝类摄食率影响因子研究现状[J]. 海洋水产研究, 2006, 27(1):76-80.
[12]	栗志民, 刘志刚, 徐法军, 等. 温度、盐度、pH和饵料密度对皱肋文蛤清滤率的影响[J]. 渔业科学进展, 2011, 32(4):55-61.
[13]	刘广丰, 沈和定, 陈慧, 等. 不同微藻对缢蛏稚贝摄食和生长的影响[J]. 上海海洋大学学报, 2009, 18(6):708-714.
[14]	张继红, 方建光. 海洋双壳贝类滤水率测定方法概述[J]. 海洋水产研究, 2005, 26(1):86-93.
[15]	冉福, 沈和定, 吕凛, 等. 文蛤对四种单胞藻的表观消化状况及同化率[J]. 上海水产大学学报, 2007, 16(6):602-607.
[16]	解万翠, 卞中园, 杨锡洪, 等. 微小亚历山大藻对近江牡蛎清滤率以及麻痹性贝类毒素(PSP)蓄积的影响[J]. 水产学报, 2013, 37(12):1848-1853.
[17]	Murray S A, O’Connor W A, Alvin A, et al. Differential accumulation of paralytic shellfish toxins from Alexandrium minutum in the pearl oyster, Pinctada imbricata[J]. Toxicon, 2009, 54(3): 217-223. DOI URL
[18]	朱明远, 邹迎麟, 吴荣军, 等. 栉孔扇贝体内麻痹性贝毒的累积与排出过程研究[J]. 海洋学报, 2003, 25(2):75-83.
[19]	黄德强, 暨卫东, 高亚辉, 等. 翡翠贻贝对塔玛亚历山大藻的摄食及毒素积累的初步研究[J]. 台湾海峡, 2003, 22(4):426-430.
[20]	Bricelj V M, Cembella A D, David L, et al. Comparative physiological and behavioral responses to PSP toxins in two bivalve molluscs, the softshell clam, Mya arenaria, and surfclam, Spisula solidissima[M]// Yasumoto T, Osima Y, Fukuyo Y. Harmful and Toxic Algal Blooms. Japan: The United Nations Educational, Scientific and Cultural Organization, 1996: 405-408.
[21]	傅萌, 颜天, 周名江. 麻痹性贝毒对海洋贝类的影响及加速贝毒净化的研究进展[J]. 水产学报, 2000, 24(4):382-387.
[22]	Guéguen M, Bardouil M, Baron R, et al. Detoxificationof Pacific oyster Crassostrea gigas fedondiets of Skeletonema costatum with and without silt, following PSP contamination by Alexandrium minutum[J]. Aquatic Living Resources, 2008, 21(1): 13-20. DOI URL
[23]	Bricelj V M, Lee J H, Cembella A D, et al. Uptake kinetics of paralytic shellfish toxins from the dinoflagellate Alexandrium fundyense in the mussel Mytilus edulis[J]. Marine Ecology Progress Series, 1990, 63(2-3): 177-188. DOI URL
[24]	Contreras A M, Marsden I D, Munro M H G. Effectsof short-term exposure to paralytic shellfish toxins on clearance rates and toxin uptake in five species of New Zealand bivalve[J]. Marine and Freshwater Research, 2012, 63(2): 166-174. DOI URL
[25]	De Carvalho I L, Pelerito A, Ribeiro I, et al. Paralytic shellﬁsh poisoning due to ingestion of contaminated mussels: a 2018 case report in Caparica (Portugal)[J]. Toxicon: X, 2019, 4: 100017. DOI URL
[26]	付家想, 蓝文陆, 李天深, 等. 香港巨牡蛎对3种浮游植物摄食率和滤清率的研究[J]. 海洋学报, 2017, 39(8):62-69.
[27]	蓝文陆, 付家想, 杨斌, 等. 香港巨牡蛎对3种微藻的选择性摄食研究[J]. 海洋学报, 2018, 40(8):79-88.
[28]	蓝文陆, 付家想, 李天深, 等. 香港巨牡蛎对不同密度2种浮游植物的摄食研究[J]. 广西科学, 2018, 25 (2):189-196.
[29]	朱雨瑞, 徐继林, 严小军. 5种微藻对4种滩涂贝类稚贝生长的影响[J]. 海洋学研究, 2010, 28(3):60-66.
[30]	Brogueira M J, do Rosário Oliveira M, Cabeçadas G. Phytoplankton community structure defined by key environmental variables in Tagus estuary, Portugal[J]. Marine Environmental Research, 2007, 64(5): 616-628. PMID
[31]	卢钰博. 牡蛎养殖区生态环境调查及影响单体三倍体牡蛎生长的因素[D]. 烟台: 烟台大学, 2021.