鱼类肠道微生物多样性及其与环境因子关系的研究进展

doi:10.14012/j.cnki.fjsc.2022.02.010

摘要/Abstract

摘要： 早期认为鱼类肠道中存在寥寥可数、结构简单的微生物群落，随着鱼类肠道微生态学的发展，人们逐渐认识到鱼类肠道中存在着种类繁多、数量庞大的微生物群落。复杂的生活环境、不同的发育阶段、多变的食物来源等因素都会导致鱼类肠道微生物多样性及结构发生变化。鱼类肠道微生物与栖息环境所形成的微生态系统在保持动态平衡时，能够维持宿主的肠道健康。大量研究显示稳态中的微生态系统能够调节肠道微生物群落的平衡、改善肠道微生物群落的组成，从而对鱼类生长发育、营养代谢、免疫调控等过程具有促进作用。本文综述了鱼类肠道微生物的研究方法、多样性和功能，总结了温度、盐度及水体饵料等环境因子对鱼类肠道微生物的影响，以期为今后鱼类肠道微生物的研究发展提供启发和参考。

关键词: 鱼类, 肠道微生物, 环境因子, 多样性, 功能

Abstract: In the early days, it was thought that there were few simple microbial communities in the fish gut, but with the development of fish gut microecology, it was gradually recognized that there were a large number of diverse microbial communities in the fish gut. Complex living environments, different developmental stages, and variable food sources can lead to changes in the diversity and structure of fish gut microorganisms. The microecosystem formed by fish gut microbes and their habitat is able to maintain the host's gut health when it is in dynamic balance. Numerous studies have shown that microecosystem in homeostasis can regulate the balance of gut microbial communities and improve the composition of gut microbial communities, thus promoting fish growth and development, nutrient metabolism, immune regulation and other processes. This paper reviewed the research methods, diversity and functions of fish gut microorganisms, and summarized the effects of environmental factors such as temperature, salinity and food in water on fish gut microorganisms, in order to provide inspiration and reference for future research development of fish gut microorganisms.

Key words: fish, gut microorganisms, environmental factors, diversity, function

中图分类号:

Q938.1

向佳丽李渊宋普庆刘世刚王芮李海妙星张然林龙山. 鱼类肠道微生物多样性及其与环境因子关系的研究进展[J]. 渔业研究, 2022, 44(2): 187-195.

参考文献

[1]杜建国，叶观琼，周秋麟，等.近海海洋生态连通性研究进展[J].生态学报, 2015, 35(21):6923-6933
[2]Wong S，Rawls J F.Intestinal microbiota composition in fishes is influenced by host ecology and environment[J].Molecular Ecology, 2012, 21(13):3100-3102
[3] 杨彬彬，邵庆均.鱼类肠道微生物的研究进展[J].中国饲料, 2013, (23):1-4
[4]Eisenstein M.The hunt for a healthy microbiome[J].Nature, 2020, 577(7792):S6-S8
[5] 郭倩倩，胡军娜，魏金锁，等.水产动物肠道微生物的研究方法[J].河南水产, 2019, 1:17-20
[6]Torsvik V，Ovreas L.Microbial diversity and function in soil：from genes to ecosystems[J].Current Opinion in Microbiology, 2002, 5(3):240-245
[7]Eckburg P B，Bik E M，Bernstein C N，et al.Diversity of the human intestinal microbial flora[J].Science, 2005, 308(5728):1635-1638
[8]冯丹，高小迪，李云凯.海洋鱼类肠道微生物研究进展及应用前景[J].生态学杂志, 2021, 40(1):255-265
[9]赵蕊，陈茂伟.肠道微生物组的研究方法进展[J].生物医学工程学杂志, 2015, 32(5):1150-1154
[10]杨翠，李祖明.分子生物学技术在肠道微生物研究中的应用进展[J].中国微生态学杂志, 2017, 29(2):229-238
[11]Roh S W，Abell G C J，Kim K H，et al.Comparing microarrays and next-generation sequencing technologies for microbial ecology research[J].Trends in Biotechnology, 2010, 28(6):291-299
[12]油九菊，柳敏海，殷小龙，等.基于高通量测序的东海带鱼肠道菌群结构分析[J].渔业研究, 2018, 40(6):434-440
[13] 孙永旭，董宏标，段亚飞，等.鱼类肠道应激及其损伤防护研究进展[J].海洋湖沼通报, 2019, 3:174-183
[14] 童雨心，梁迎春.肠道微生物与人体健康研究进展[J].生物技术通讯, 2014, 6:896-900
[15]Hansen G H，Olafsen J A.Bacterial colonization of cod(Gadus morhua L)and halibut(Hippoglossus hippoglossus)eggs in marine aquaculture[J].Applied and Environmental Microbiology, 1989, 55(6):1435-1446
[16]Roeselers G，Mittge E K，Stephens W Z，et al.Evidence for a core gut microbiota in the zebrafish[J].The ISME journal, 2011, 5(10):1595-1608
[17] 王文娟，潘宝海，孙冬岩，等.水产动物肠道菌群的形成及其生理作用[J].饲料研究, 2012, 2:37-39
[18]Nayak SK.Role of gastrointestinal microbiota in fish[J].Aquaculture Research, 2010, 41(11):1553-1573
[19]罗亮，赵志刚，都雪，等.鱼类肠道菌群与免疫力的相关性研究[J].水产学杂志, 2018, 31(6):51-54
[20]Nayak S K.Role of gastrointestinal microbiota in fish[J].Aquaculture Research, 2010, 41(11):1553-1573
[21]孟晓林，李文均，聂国兴.鱼类肠道菌群影响因子研究进展[J].水产学报, 2019, 43(1):143-155
[22] 翟万营，郭安宁.鱼类肠道微生物研究进展[J]. ，（4）：18-21.[J].河南水产, 2016, (4):18-21
[23] Givens C E，Ransom B，Bano N，et al.Comparison of the gut microbiomes of 12 bony fish and 3 shark species[J]. Marine Ecology Progress, 2015, 518:209-223
[24]马阿敏，李娜，覃虹焱，等.淡水鱼类肠道微生物菌群研究进展[J].甘肃畜牧兽医, 2021, 51(5):9-13
[25] 祭仲石.三种不同环境中鲢鳙肠道微生物研究[D]. 上海：上海海洋大学，2014.
[26]黄丽丽，孙海龙，倪永清，等.新疆额尔齐斯河冷水性鲤鱼肠道细菌多样性分析[J].中国食品学报, 2019, 19(11):246-254
[27] Gajardo K，Rodiles A，Kortner T M，et al.A high-resolution map of the gut microbiota in Atlantic salmon（Salmo salar）：a basis for comparative gut microbial research[J]. Scientific Reports，2016，6：30893.[J].Scientific Reports, 2016, 6(1):30893-
[28] 席晓晴.分子技术在马鞍列岛海域鱼类食性和肠道菌群分析中的应用[D]. 上海：上海海洋大学，2016.
[29] 马英，王程程，沈晓群，等.大黄鱼肠道细菌分离培养及分子鉴定[C]. 中国海洋湖沼学会鱼类学分会、中国动物学会鱼类学分会2012年学术研讨会论文摘要汇编. 青岛：中国海洋湖沼学会，2012，116-117.
[30]Gatesoupe F J.Live yeasts in the gut：natural occurrence，dietary introduction，and their effects on fish health and development[J].Aquaculture, 2007, 267(1):20-30
[31] 朱国平，王敏.南极海洋生物肠道微生物研究进展[J].生态学报, 2021, 21:1-11
[32] 陈孝煊，吴志新，周文豪.鱼类消化道菌群的作用与影响因素研究进展[J].华中农业大学学报, 2005, 5:101-106
[33]Clements K D，Angert E R，Montgomery W L，et al.Intestinal microbiota in fishes：what’s known and what’s not[J].Molecular Ecology, 2014, 23(8):1891-1898
[34] 吴杰奇，曾发姣，雷平，等.娄底地区鱼类肠道微生物与酶活性的初步研究[J].湖南农业科学, 2020, 4:55-57
[35]侯进慧，陈宏伟，曹泽虹，等.鲫鱼肠道细菌菌群初步分析[J].食品科学, 2010, 31(11):178-181
[36]Nishida A，Inoue R，Inatomi O，et al.Gut microbiota in the pathogenesis of inflam matory bowel disease[J].Clinical Journal of Gastroenterology, 2018, 11(1):1-10
[37]Kudva A K，Shay A E，Prabhu K S.Selenium and inflammatory bowel disease[J].American Journal of Physiology. Gastrointestinal and Liver Physiology, 2015, 309(2):G71-G77
[38] Chen Y J，Yuan R M，Liu Y J，et al.Dietary vitamin C requirement and its effects on tissue antioxidant capacity of juvenile largemouth bass，Micropterus salmoides[J].Aquaculture, 2015, 435:431-436
[39]Semova I，Carten J D，Stombaugh J，et al.Microbiota regulate intestinal absorption and metabolism of fatty acids in the zebrafish[J].Cell Host & Microbe, 2012, 12(3):277-288
[40] 迟骋.鲤肠道优势菌对其免疫功能的影响[D]. 咸阳：西北农林科技大学，2014.
[41] 王融，包栩睿，王鑫钰，等.鱼类肠道乳酸菌的抑菌特性及机理研究[J].现代化农业, 2014, 8:49-51
[42]Sugita H，Shibuya K，Shimooka H，et al.Antibacterial abilities of intestinal bacteria in freshwater cultured fish[J].Aquaculture, 1997, 4(1):195-203
[43]熊向英，王贤丰，彭银辉，等.健康和患病卵形鲳鲹肠道菌群结构的差异[J].水产学报, 2019, 43(5):1317-1325
[44]Pan C，Huang T，Wang Y，et al.Oral administration of recombinant epinecidin-1 protected grouper(Epinephelus coioodes)and zcbrafish(Danio rerio)from Vibro vulnificus infection and enhanced immunerelated gene expressions[J].Fish ＆ Shellfish lmmunology, 2012, 32(6):947-957
[45] 吕欣荣，肖克宇.鱼类肠道菌群的研究现状[J].江西水产科技, 2008, 2:12-18
[46]张燕玉，韩卓然，孙敬锋，等.海藻希瓦氏菌感染对半滑舌鳎肠道菌群结构及相关功能基因表达的影响[J].南方农业学报, 2019, 50(10):2300-2307
[47]Huys L，Dhert P H，Robles R，et al.Search for beneficial bacterial strains for turbot (Scophthalmus maximus L)larviculture[J].Aquaculture, 2001, 193(1):25-37
[48] 杜东东.盐单胞菌PHB对珍珠龙胆石斑鱼生长和免疫影响的研究[D]. 天津：天津科技大学，2020.
[49]Spanggaard B，Huber I，Nielsen J，et al.The microflora of rainbow trout intestine：a comparison of traditional and molecular identification[J].Aquaculture, 2000, 182(1):1-15
[50]Navarrete P，Mardones P，Opazo R，et al.Oxytetracycline treatment reduces bacterial diversity of intestinal microbiota of Atlantic salmo[J].Journal of Aquatic Animal Health, 2008, 20(3):177-183
[51] Jones J，Dibattista J D，Stat M，et al.The microbiome of the gastrointestinal tract of a range-shifting marine herbivorous fish[J]. Frontiers in Microbiology，2018，9：1-13.[J].Frontiers in Microbiology, 2018, 9:1-13
[52] 曾楠楠.不同水温对吉富罗非鱼的饲料蛋白质需要量、生理代谢及肠道微生物的影响[D]. 上海：上海海洋大学，2020.
[53] 汪倩，翟万营，韩爽，等.低温胁迫对暗纹东方鲀肠道微生物群落结构的影响[J/OL]. 大连海洋大学：1-17[2021-11-21]. https://doi.org/10.16535/j.cnki.dlhyxb.2021-059.[J].大连海洋大学, 2021, :-
[54]Alharbi A H，Uddin M N.Seasonal variation in the intestinal bacterial flora of hybrid tilapia(Oreochromis niloticus x Oreochromis aureus)cultured in earthen ponds in Saudi Arabia[J].Aquaculture, 2004, 229(1):37-44
[55] 毛婕.东海区黄鳍东方鲀毒性及肠道内食源性物种季节分析[D]. 上海：上海海洋大学，2019.
[56] 郭倩倩.水体铜暴露对鲤肠道微生物群落结构及机体脂代谢的影响[D]. 新乡：河南师范大学，2016.
[57] 邵仙萍.碱式氯化铜对异育银鲫和团头鲂生长、组织铜水平、抗氧化活性及肠道菌群的影响[D]. 南京：南京农业大学，2012.
[58] Merrifield D L，Shaw B J，Harper G M，et al.Ingestionof metal-nanoparticle contaminated food disruptsendogenous microbiota in zebrafish（Danio rerio）[J].Environmental Pollution, 2013, 174:157-163
[59] Shimizu Y，Hajima T，Fujita Y，Omasa K.Assessments of climate change impacts on the terrestrial ecosystem in Japan using the Bio-Geographical and GeoChemical（BGGC）model[J].Journal of Agricultural Meteorology, 2005, 60:5-10
[60] 田璐.盐度对黄姑鱼生存生长、非特异性免疫及肠道菌群的影响[D]. 舟山：浙江海洋大学，2019.
[61] Miyake S，Ngugi D K，Stingl U.Diet strongly influences the gut microbiota of surgeonfishes[J].Molecular Ecology, 2015, 24:656-672
[62]钟蕾，向建国，曾丹，等.饵料对鳡肠道微生物多样性的影响[J].水生生物学报, 2016, 40(4):830-835
[63]孔祎頔，田佳鑫，陈秀梅，等.水产动物益生菌及其对肠道菌群影响的研究进展[J].中国畜牧杂志, 2019, 55(1):29-33
[64] 王纯.芽孢杆菌V4和胶红酵母对鲑鳟鱼生长免疫及肠道菌群影响研究[D]. 北京：中国科学院大学（中国科学院海洋研究所），2017.
[65]张书环，吴金平，褚志鹏，等.饲喂枯草芽孢杆菌对杂交鲟生长和肠道菌群结构的影响[J].海洋渔业, 2021, 43(1):71-80
[66] Rimmer D W.Changes in diet and the development of microbial digestion in juvenile buffalo bream，Kyphosus cornelii[J].Marine Biology, 1986, 92:443-448
[67]Moran D，Turner S J，Clements K D.Ontogenetic development of the gastrointestinal microbiota in the marine herbivorous fish Kyphosus sydneyanus[J].Microbial Ecology, 2005, 49(4):590-597
[68] 李武辉.鲂鲌品系和鲌鲂品系遗传特性及食性研究[D]. 长沙：湖南师范大学，2018.
[69] 刘妮.玫瑰高原鳅食性分析[D]. 重庆：西南大学，2020.
[70]Moline M A，Prézelin B B.Long?term monitoring and analyses of physical factors regulating variability in coastal Antarctic phytoplankton biomassin situ productivity and taxonomic composition over subseasonal，seasonal and interannual time scales[J].Marine Ecology Progress Series, 1996, 145(1):143-160
[71]Shevenell A E，Kennett J P，Lea D W.Middle miocene Southern Ocean cooling and Antarctic cryosphere expansion[J].Science, 2004, 305(5691):1766-1770
[72] 翟万营.南极鱼亚目鱼类肠道、皮肤黏液及其生存水环境微生物结构和功能研究[D]. 上海：上海海洋大学，2020.